Plant Protect. Sci., 2022, 58(4):338-350 | DOI: 10.17221/130/2021-PPS

Evaluation of genetic and induced resistance phenomena in cucumbers against the root-knot nematode (Meloidogyne incognita)Original Paper

Rana Muhammad Amir Gulzar ORCID...1,2, Ateeq Ur Rehman ORCID...2, Ummad Ud Din Umar ORCID...*,2, Muhammad Shahid3, Muhammad Fahad Khan ORCID...2
1 Laboratory of Molecular Biology of Plant Disease Resistance, Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, P.R. China
2 Department of Plant Pathology, Bahauddin Zakariya University, Multan, Pakistan
3 Plant Pathological Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan

Meloidogyne spp. is an important pest of cucurbits in tunnel farming of vegetables in Pakistan. A cucumber germplasm was evaluated for resistance against the root-knot nematode (RKN, Meloidogyne incognita) based on the number of galls and egg masses recorded under glass house conditions. All the cucumber accessions showed varying responses towards the RKN inoculation. Out of the fifteen cucumber lines, two were found to be moderately susceptible to M. incognita, ten were susceptible while the other three were highly susceptible. For the management of the nematodes, resistance was induced in a highly susceptible cucumber accession (28294) by the application (both drench and foliar) of elicitors, i.e., salicylic acid (SA) and benzothiadiazole (BTH). The application of SA and BTH significantly enhanced the defence mechanism of the cucumber plants when compared to the control. Increased enzymatic activities in the cucumber plants as the result of the elicitor applications were determined through spectrophotometer to correlate the induced resistance. There was a significant increase in the enzymatic activities when compared to the control in the cucumber plants, which ultimately enhanced the resistance as there was a decrease in the number of galls and egg masses per plant. The enzymatic antioxidant activity was also found to increase in response to the nematode infection. Both SA and BTH were reported to play pivotal roles in inducing resistance in cucumber plants against M. incognita.

Keywords: Cucumis sativus; M. incognita; SAR; benzothiadiazole; salicylic acid; enzymatic activities

Published: October 3, 2022  Show citation

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Gulzar RMA, Rehman AU, Umar UUD, Shahid M, Khan MF. Evaluation of genetic and induced resistance phenomena in cucumbers against the root-knot nematode (Meloidogyne incognita). Plant Protect. Sci. 2022;58(4):338-350. doi: 10.17221/130/2021-PPS.
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    References

    1. Abad P., Favery B., Rosso M.N., Castagnone-Sereno P. (2003): Root-knot nematode parasitism and host response: Molecular basis of a sophisticated interaction. Molecular Plant Pathology, 4: 217-224. Go to original source... Go to PubMed...
    2. Achuo A.E., Audenaert K., Meziane H., Höfte M. (2002): The SA-dependent defense pathway is active against different pathogens in tomato and tobacco. Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen), 67: 149-157.
    3. Aebi H. (1984): Catalase in vitro. In: Jura N., Murphy J.M. (eds). Methods in Enzymology. Waltham, Elsevier Academic Press: 121-126. Go to original source...
    4. Anwar S.A., Zia A., Hussain M., Kamran M. (2007): Host susceptibility of selected plants to Meloidogyne incognita in the Punjab, Pakistan. International Journal of Nematology, 17: 144-150.
    5. Bajestani M.S., Moghadam E.M., Aghnoum R., Rohani H. (2019): Genotypic and biochemical variation in the response of barley to the Root-knot nematode (Meloidogyne javanica) at seedling stage. Pakistan Journal of Phytopathology, 31: 7-17. Go to original source...
    6. Bello T.T., Coyne D.L., Fourie H. (2021): Reproduction potential of Nigerian Meloidogyne spp. and the response of six commercial watermelon cultivars to predominant species. Journal of Plant Diseases and Protection, 128: 831-842. Go to original source...
    7. Bradford M.M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Analytical Biochemistry, 72: 248-254. Go to original source...
    8. Burketová L., ©indelářová M., ©indelář L. (1999): Benzothiadiazole as an inducer of β-1,3-glucanase and chitinase isozymes in sugar beet. Biologia Plantarum, 42: 279-287. Go to original source...
    9. Chance B., Maehly A.C. (1955): Assay of catalase and peroxidase. Methods in Enzymology, 2: 764-775. Go to original source...
    10. Clarke C.R., Vinatzer B.A. (2017): Characterizing the immune-eliciting activity of putative microbe-associated molecular patterns in tomato. In: Shan L., He P. (eds). Plant Pattern Recognition Receptors. Methods and Protocols. New York, Springer: 249-261. Go to original source...
    11. Dickerson D.P., Pascholati S.F., Hagerman A.E., Butler L.G., Nicholson R.L. (1984): Phenylalanine ammonia-lyase and hydroxycinnamate CoA ligase in maize mesocotyls inoculated with Helminthosporium maydis or Helminthosporium carbonum. Physiology Plant Pathology, 25: 111-123. Go to original source...
    12. Eder A.B., Mauro F.B., Carlos B.J., Gilbert E.J., Thales R., Janice D.A.E. (2018): Imaging mass spectrometry of endogenous polypeptides and secondary metabolites from galls induced by root knot nematodes in tomato roots. Journal of Molecular Plant Microbe Interactions, 31: 1048-1059. Go to original source... Go to PubMed...
    13. Eder R., Consoli E., Krauss J., Dahlin P. (2021): Polysulfides applied as formulated garlic extract to protect tomato plants against the root-knot nematode Meloidogyne incognita. Plants, 10: 394. doi: 10.3390/plants10020394 Go to original source... Go to PubMed...
    14. Elekcioglu I.H., Ohnesorge B., Lung G., Uygun N. (1994): Plant parasitic nematodes in the Mediterranean region of Turkey. Nematol Mediterranian, 22: 59-63.
    15. Giannopolitis C.N., Ries S.K. (1997): Superoxide dismutase I. Occurrence in higher plants. Plant Physiology, 59: 309-314. Go to original source... Go to PubMed...
    16. Holbrook C.C., Knauft D.A., Dickson D.W. (1983): A technique for screening peanut for resistance to Meloidogyne arenaria. Plant Disease, 57: 957-958. Go to original source...
    17. Janati S., Houari A., Wifaya A., Essarioui A., Mimouni A., Hormatallah A., Sbaghi M., Dababat A.A., Mokrini F. (2018): Occurrence of the root-knot nematode species in vegetable crops in Souss region of Morocco. The Plant Pathology Journal, 34: 308-315. Go to original source... Go to PubMed...
    18. Jones J.T., Haegeman A., Danchin E.G., Gaur H.S., Helder J., Jones M.G. (2013): Top 10 plant-parasitic nematodes in molecular plant pathology. Molecular Plant Pathology, 14: 946-961. Go to original source... Go to PubMed...
    19. Kammerhofer N., Radakovic Z., Regis J.M., Dobrev P., Vankova R., Grundler F.M. (2015): Role of stress-related hormones in plant defence during early infection of the cyst nematode Heterodera schachtii in Arabidopsis. New Phytology, 207: 778-789. Go to original source... Go to PubMed...
    20. Kaskavalci G. (2007): Effect of soil solarization and organic amendments treatment for controlling Meloidogyne incognita in tomato cultivars in Western Anatolia. Turkish Journal of Agriculture and Forestry, 31: 159-167.
    21. Kassi A.H., Hussain S.I. (1987): Screening of some tomato cultivars for their resistance to Meloidogyne javanica under Iraqi conditions. International Nematology Network Newsletter, 4: 27-29.
    22. Kayani M.Z., Mukhtar T., Hussain M.A. (2017): Effects of southern root knot nematode population densities and plant age on growth and yield parameters of cucumber. Crop Protection, 92: 207-212. Go to original source...
    23. Khan M.T., Mukhtar T., Saeed M. (2019): Resistance or susceptibility of eight aubergine cultivars to Meloidogyne javanica. Pakistan Journal of Zoology, 51: 2187-2192. Go to original source...
    24. Kyndt T., Nahar K., Haeck A., Verbeek R., Demeestere K., Gheysen G. (2017): Interplay between carotenoids, abscisic acid and jasmonate guides the compatible rice-Meloidogyne graminicola interaction. Frontiers in Plant Science, 8: 951. doi: 10.3389/fpls.2017.00951 Go to original source... Go to PubMed...
    25. Mandal S. (2010): Induction of phenolics, lignin and key defense enzymes in eggplant (Solanum melongena L.) roots in response to elicitors. African Journal of Biotechnology, 9: 8038-8047. Go to original source...
    26. Moghbeli E., Nemati S.H., Aroiee H., Olfati J.A. (2017): Evaluation of resistance, enzymatic response, and phenolic compounds in roots of F1 cucumber hybrids to Fusarium oxysporum f. sp. radicis-cucumerinum. Journal of Horticulture Research, 25: 117-124. Go to original source...
    27. Molinari S. (2001): Inhibition of H2O2-degrading enzymes in the response of Mi-bearing tomato to root-knot nematodes and salicylic acid treatment. Nematologia Mediterranea, 29: 235-239.
    28. Molinari S. (2008): Salicylic acid as an elicitor of resistance to root-knot nematodes in tomato. Acta Horticulture, 789: 119-126. Go to original source...
    29. Moussa F.F., Elgindi D.M., Kheir A.M., Koraiem A.M. (1981): Reaction of certain cucurbit cultivars to nine populations of Meloidogyne incognita. Bulletin of Zoological Society Egypt, 31: 93.
    30. Mukhtar T., Kayani M.Z., Hussain M.A. (2013): Response of selected cucumber cultivars to Meloidogyne incognita. Crop Protection, 44: 13-17. Go to original source...
    31. Nahar K., Kyndt T., De Vleesschauwer D., Hofte M., Gheysen G. (2011): The jasmonate pathway is a key player in systemically induced defense against root knot nematodes in rice. Plant Physiology, 157: 305-316. Go to original source... Go to PubMed...
    32. Nandi B., Nandy R.K., Mukhopadhyay S., Nair G.B., Shimada T., Ghose A.C. (2000): Rapid method for species-specific identification of Vibrio cholerae using primers targeted to the gene of outer membrane protein OmpW. Journal of Clinical Microbiology, 38: 4145-4151. Go to original source... Go to PubMed...
    33. Nguyen D.M.C., Seo D.J., Park R.D. (2011): Changes in antioxidative enzyme activities in cucumber plants with regard to biological control of root-knot nematode, Meloidogyne incognita with Cinnamomum cassia crude extracts. Journal of Korean Society and Applied Biological Chemistry, 54: 507-514. Go to original source...
    34. Pasqua V., Costantino P., Federico P., Silvana D.L., Alba G.U., Maria T.M. (2018): Changes in lignin biosynthesis and monomer composition in response to benzothiadiazole and root-knot nematode Meloidogyne incognita infection in tomato. Journal of Plant Physiology, 230: 40-50. Go to original source... Go to PubMed...
    35. Punithaveni V., Jansirani P., Sivakumar M. (2015): Screening of cucurbitaceous rootstocks and cucumber scions for root knot nematode resistance (Meloidogyne incognita Kofoid and White). Electronic Journal of Plant Breeding, 6: 486-492.
    36. Qi W., Ke C., Haiyan L., Gengrui Z., Weichao F., Changwen C., Xinwei W., Lirong W. (2018): Lignification plays an important role on resistance to root-knot nematode (Meloidogyne incognita) based on contrastive analysis in peach. Scientia Horticulturae, 238: 1-6. Go to original source...
    37. Sasanelli N., Konrat A., Migunova V., Toderas I., IurcuStraistaru E., Rusu S., Bivol A., Andoni C., Veronico P. (2021): Review on control methods against plant parasitic nematodes applied in southern member states (C Zone) of the European Union. Agriculture, 11: 602. doi: 10.3390/agriculture11070602 Go to original source...
    38. Sasser J.N., Freckman D.W. (1987): World perspective of Nematology. The role of society. In: Veech J.A., Dickson D.W. (eds). Vistas on Nematology: A Commemoration of the Twenty-Fifth Anniversary of the Society of Nematologist. Hyattville, Society of Nematologist, Inc.: 7-14.
    39. Sharma S., Sohal B.S. (2010): Foliar spray of benzothiadiazole and salicylic acid on Brassica juncea var. RLM619 to combat Alternaria blight in field trials. Crop Improvement, 31: 87-92.
    40. Singh U.B., Singh S., Khan W., Malviya D., Sahu P.K., Chaurasia R., Sharma S.K., Saxena A.K. (2019): Drechslerella dactyloides and Dactylaria brochopaga mediated induction of defense related mediator molecules in tomato plants pre-challenged with Meloidogyne incognita. Indian Phytopathology, 72: 309-320. Go to original source...
    41. Song L., Xu X.C., Wang F.N., Wang Y., Xia X.J., Shi K. (2017): Brassino steroids act as a positive regulator for resistance against root-knot nematode involving respiratory burst oxidase homolog-dependent activation of MAPKs in tomato. Plant Cell Environment, 41: 1113-1125. Go to original source... Go to PubMed...
    42. Taylor D.P., Nestscher C. (1974): An improved technique for preparing perennial pattern of Meloidogyne spp. Nematologica, 20: 268-269. Go to original source...
    43. Vimala R., Suriachandraselvan M. (2009): Induced resistance in bhendi against powdery mildew by foliar application of salicylic acid. Journal of Biopesticides, 2: 111-114. Go to original source...
    44. Walter S.A., Wehner T.C., Barkel K.R. (1993): Root-knot nematode resistance in cucumber and horned cucumber. Horticulture Science, 28: 151-154. Go to original source...
    45. Whitehead A.G., Hemming J.R. (1965): A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology, 55: 25-38. Go to original source...
    46. Wright D.J. (1981): Nematicides: Mode of action and new approaches to chemical control. In: Zuckerman B.M., Rohde R.A. (eds). Plant-Parasitic Nematodes. London and New York, Academic Press: 421-449. Go to original source...
    47. Yigezu Wendimu G. (2021): Biology, taxonomy, and management of the root-knot nematode (Meloidogyne incognita) in sweet potato. Advances in Agriculture, 2021: 8820211. doi: 10.1155/2021/8820211 Go to original source...
    48. Yuksel H. (1974): Considerations on the state of root knot nematodes (Meloidogyne spp.) in Turkey and their population problems. Journal of Agricultural Faculty of Atatürk University, 5: 83-105.
    49. Zieslin N., Ben-Zaken R. (1993): Peroxidase activity and presence of phenolic substances in peduncles of rose flowers. Plant Physiology and Biochemistry, 31: 333-339.
    50. Zinovieva S.V., Vasyukova N.I., Udalova Z.V., Gerasimova N.G. (2013): The participation of salicylic and jasmonic acids in genetic and induced resistance of tomato to Meloidogyne incognita. Biology Bulletin, 40: 297-303. Go to original source...

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