Plant Protect. Sci., 2023, 59(2):124-133 | DOI: 10.17221/45/2022-PPS

Molecular diagnosis of red rot of sugarcane in Pakistan and in vitro control of isolated pathogen using rhizobacteriaOriginal Paper

Zimen Ahmad1, Muhammad Asad Farooq3, Amama Fatima2, Syeda Gul Nisa1, Sania Tabassum2, Bushra Ismat2, Sundus Waseem2, Umer Iqbal3, Asma Akbar3, Urooj Haroon1, Hassan Javed Chaudhary1, Tauseef Tabassum3, Ahmed Z Dewidar4, Abdullah Alaklabi5, Muhammad Farooq Hussain Munis ORCID...1
1 Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan
2 Department of Plant Pathology, Faculty of Crop and Food Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
3 Crop Disease Research Institute, National Agricultural Research Centre, Islamabad, Pakistan
4 Prince Sultan Institute of Environmental, Water and Desert Research, King Saud University, Riyadh, Saudi Arabia
5 Department of Biology, Faculty of Science, University of Bisha, Bisha, Saudi Arabia

Sugarcane (Saccharum officinarum L.) is a key cash crop, and its production is influenced by a variety of phytopathogens in different parts of the world. During consecutive field surveys, sugarcane stalks with red rot symptoms were observed in three provinces of Pakistan (Punjab, Sindh, and Khyber Pakhtunkhwa). Cane samples with visible symptoms were plated on Potato Dextrose Agar (PDA) media. Morphological and microscopic observations identified this pathogen as Colletotrichum falcatum. Comparative molecular study of amplified sequences of β-tubulin (Bt) and Actin (ACT) genes showed 100% similarity and ITS sequence showed 99% similarity with C. falcatum. For in vitro control of red rot, eight bacterial strains from the rhizosphere of sugarcane were isolated. These strains showed variable growth inhibition of C. falcatum in dual culture method. Among all tested strains, Bacillus amyloliquefaciens and B. altitudinis exhibited best antifungal activities. Based on these results, tested bacterial strains (B. amyloliquefaciens and B. altitudinis) can be recommended as effective biocontrol agents to manage red rot disease of sugarcane.

Keywords: sugarcane; Colletotrichum falcatum; ITS; rDNA; Bacillus

Published: May 17, 2023  Show citation

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Ahmad Z, Farooq MA, Fatima A, Nisa SG, Tabassum S, Ismat B, et al.. Molecular diagnosis of red rot of sugarcane in Pakistan and in vitro control of isolated pathogen using rhizobacteria. Plant Protect. Sci. 2023;59(2):124-133. doi: 10.17221/45/2022-PPS.
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