Plant Protect. Sci., 2023, 59(4):389-393 | DOI: 10.17221/70/2023-PPS

Detection and molecular identification of ‘Candidatus Phytoplasma asteris’ associated with muscari virescence of three grape hyacinth speciesShort Communication

Emese Mergenthaler ORCID...1, József Fodor ORCID...1, János Ágoston ORCID...2, Orsolya Viczián ORCID...1
1 Plant Protection Institute, Centre for Agricultural Research, ELKH, Budapest, Hungary
2 ELKH-SZE PhatoPlant-Lab, Széchenyi István University, Mosonmagyaróvár, Hungary

Grape hyacinths are popular perennial, flowering bulbous plants. In 2018, 2019, and 2020, some plants of three Muscari species showed symptoms similar to those associated with phytoplasma infection in commercial ornamental gardens in Hungary. Symptoms included virescence of flowers and yellowing of leaves. Symptomatic and asymptomatic Muscari plants were sampled at two locations to detect and identify the pathogens involved. Total DNA was extracted from the plants and used as a template in polymerase chain reaction assays to amplify 16S rRNA gene sequences and housekeeping genes (tuf, secY) with phytoplasma-specific primers. The resulting PCR products from symptomatic plants were cloned and sequenced bidirectionally. Homology searching of the obtained sequences against the GenBank database indicated the presence of  'Candidatus Phytoplasma asteris' in the three Muscari species. This is the first report worldwide of C. P. asteris phytoplasma infecting M. botryoides and M. comosum, and its first identification in M. armeniacum in Hungary.

Keywords: grape hyacinths, phytoplasma, virescence, aster yellows

Received: June 9, 2023; Revised: October 2, 2023; Accepted: October 4, 2023; Prepublished online: November 16, 2023; Published: November 28, 2023  Show citation

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Mergenthaler E, Fodor J, Ágoston J, Viczián O. Detection and molecular identification of ‘Candidatus Phytoplasma asteris’ associated with muscari virescence of three grape hyacinth species. Plant Protect. Sci. 2023;59(4):389-393. doi: 10.17221/70/2023-PPS.
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    References

    1. Ahrens U., Seemüller E. (1992): Detection of DNA of plant pathogenic mycoplasma-like organism by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology, 82: 828-832. Go to original source...
    2. Bellardi M.G., Bertaccini A., Madhupriya, Rao G.P. (2018): Phytoplasma diseases in ornamental crops. In: Rao, G., Bertaccini, A., Fiore, N., Liefting, L. (eds). Phytoplasmas: Plant Pathogenic Bacteria - I. Springer, Singapore. doi: 10.1007/978-981-13-0119-3_7 Go to original source...
    3. Bertaccini A., Arocha-Rosete Y., Contaldo N., Duduk B., Fiore N., Montano H.G., Kube M., Kuo C.-H., et al. (2022): Revision of the 'Candidatus Phytoplasma' species description guidelines. International Journal of Systematic and Evolutionary Microbiology, 72: 005353. doi: 10.1099/ijsem.0.005353 Go to original source... Go to PubMed...
    4. Deng S., Hiruki C. (1991): Amplification of 16S rRNA genes from culturable and nonculturable mollicutes. Journal of Microbiological Methods, 14: 53-61. Go to original source...
    5. Dermastia M. (2019): Plant hormones in phytoplasma infected plants. Frontiers in Plant Science, 10: 1-15. Go to original source... Go to PubMed...
    6. Gundersen D.E., Lee I.M. (1996): Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathologia Mediterranea, 35: 144-151.
    7. Kaminska M. (2008): Phytoplasma in ornamental plants. In: Harrison N.A., Rao G.P., Marcone C. (eds): Characterization, Diagnosis and Management of Phytoplasmas, Studium Press LLC, Texas, USA: 195-218.
    8. Kumar S., Stecher, G., Tamura, K. (2016): MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33: 1870-1874. Go to original source... Go to PubMed...
    9. Madeira F., Pearce M., Tivey A.R.N., Basutkar P., Lee J., Edbali O., Madhusoodanan N., Kolesnikov A., et al. (2022): Search and sequence analysis tools services from EMBL-EBI in 2022. Nucleic Acids Research, 50: W276-W279. Go to original source... Go to PubMed...
    10. Marcone C., Lee I.-M., Davis R.E., Ragozzino A., Seemüller E. (2000): Classification of aster yellows-group phytoplasmas based on combined analyses of rRNA and tuf gene sequences. International Journal of Systematic Bacteriology, 50: 1703-1713. Go to original source... Go to PubMed...
    11. Samuitienė M., Jomantienė R., Valiunas D., Navalinskienė M., Davis R.E. (2007): Phytoplasma strains detected in ornamental plants in Lithuania. Bulletin of Insectology, 60: 137-138.
    12. Schneider B., Seemüller E., Smart C.D., Kirkpatrick, B.C. (1995): Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas. In: Razin S. and Tully J.G. (eds). Molecular and Diagnostic Procedures in Mycoplasmology, Vol. I. Academic Press, San Diego, 369-380. Go to original source...
    13. Schneider B., Gibb K.S. (1997): Sequence and RFLP analysis of the elongation factor Tu gene used in differentiation and classification of phytoplasmas. Microbiology, Go to original source...
    14. 143: 3381-3389.
    15. Viczián O., Fodor J., Ágoston J., Mergenthaler E. (2023): First report of 'Candidatus Phytoplasma asteris' associated with cyclamen little leaf in Hungary. Plant Disease, 107: 2515. doi: 10.1094/PDIS-12-22-2870-PDN Go to original source... Go to PubMed...
    16. Wraga K., Placek M. (2009): Review of taxons from genus Muscari cultivated in Department of Ornamental Plants in Szczecin. Herba Polonica, 55: 348-353.
    17. Zhao Y., Wei W., Lee I.M., Shao J., Suo X., Davis R.E. (2009): Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). International Journal of Systematic and Evolutionary Microbiology, 59: 2582-2593. Go to original source... Go to PubMed...

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