Plant Protect. Sci., 2023, 59(4):337-347 | DOI: 10.17221/52/2023-PPS

Identification and functional analysis of glyoxal oxidase gene from rubber tree anthracnoseOriginal Paper

Xuemei Xian, Kunmeng Li, Hui Liu, Zhenhui Wang, Kun Yuan, Yiyu Hu, Chengtian Feng, Meng Wang, Chunhua Lin, Qiguang He, Yu Zhang
1 Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China
2 Hainan Key Laboratory for Cultivation and Physiology, Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China
3 College of Tropical Crops, Yunnan Agricultural University, Puer, China

Glyoxal oxidase (GLOX, EC 1.2.3.15) is a class of hydrogen peroxide synthases involved in lignin degradation. Several GLOX genes of biotrophic and necrotrophic phytopathogenic fungi have been confirmed to contribute to fungal growth, development, and pathogenicity. However, the composition, sequence characteristics and functions of genes in the GLOX family of hemi-biotrophic phytopathogenic fungi remain poorly understood. This study identified a GLOX-encoding gene CsGLOX in the hemi-biotrophic phytopathogenic fungus Colletotrichum siamense by bioinformatics analysis and PCR cloning. The encoded protein contains a signal peptide and five cell wall stress-responsive component (WSC) domains at the N terminus, followed by a catalytic structural domain (AA5_1). CsGLOX-deficient mutants were constructed by homologous replacement, which significantly increased the formation of conidia and reduced the pathogenicity and the high-osmotic stress tolerance of the fungus. However, the deletions did not influence fungal filamentous growth. This study proposes that the CsGLOX gene is involved in pathogen conidial formation, pathogenicity, and osmotic stress response, which deepens our understanding of the pathogenic mechanism of hemi-biotrophic phytopathogenic fungus C. siamense.

Keywords: glyoxal oxidase; Colletotrichum siamense; pathogenicity; stress responses; pathogenic mechanism

Received: May 9, 2023; Revised: September 19, 2023; Accepted: October 4, 2023; Prepublished online: November 10, 2023; Published: November 28, 2023  Show citation

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Xian X, Li K, Liu H, Wang Z, Yuan K, Hu Y, et al.. Identification and functional analysis of glyoxal oxidase gene from rubber tree anthracnose. Plant Protect. Sci. 2023;59(4):337-347. doi: 10.17221/52/2023-PPS.
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