Plant Protect. Sci., 2025, 61(1):66-76 | DOI: 10.17221/44/2024-PPS

The potential of volatiles from Brassica juncea seeds against grey mould agent Botrytis cinerea and their effect on storage and sensory quality of spinach leavesOriginal Paper

Beata Kowalska ORCID...1, Magdalena Szczech ORCID...1, Maria Grzegorzewska ORCID...1, Anna Wrzodak ORCID...1, Kalina Sikorska-Zimny ORCID...1
1 The National Institute of Horticultural Research, Skierniewice, Poland

The potential use of volatile compounds released from milled seeds of mustard (Brassica juncea cv. Malopolska) obtained from three different companies was tested in in vitro and in vivo experiments for their inhibitory effect on Botrytis cinerea growth on agar media and its infection on vegetable leaves of cucumber, bean and spinach. In the experiments with spinach, the effect of volatiles from mustards on the storage and sensory quality of fumigated leaves was evaluated. The antifungal effect of the volatiles depended on the source and dosage of mustard seeds and biofumigation time. The most efficient inhibition of B. cinerea mycelium growth on agar media and vegetable leaves was mustard S from SHR company. The development of grey mould on spinach leaves was inhibited in the treatment with 4 h biofumigation with the volatiles from mustard S seeds in experiments conducted at 10 °C and also at 18 °C. In the sensory and storage quality analysis, the spinach leaves treated with volatiles from mustard seeds showed acceptable parameters that predisposed the product to consumption. The results show that it is possible to reduce the incidence of vegetable grey mould with the treatment of milled mustard seeds, opening a potential application of biofumigation in the control of B. cinerea in vegetables.

Keywords: biofumigation; mustard; grey mould; cucumber; bean

Received: March 19, 2024; Revised: October 31, 2024; Accepted: November 4, 2024; Published: January 15, 2025  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Kowalska B, Szczech M, Grzegorzewska M, Wrzodak A, Sikorska-Zimny K. The potential of volatiles from Brassica juncea seeds against grey mould agent Botrytis cinerea and their effect on storage and sensory quality of spinach leaves. Plant Protect. Sci. 2025;61(1):66-76. doi: 10.17221/44/2024-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abul-Fadl M.M., El-Badry N., Ammar M.S. (2011): Nutritional and chemical evaluation for two different varieties of mustard seeds. World Applied Sciences Journal, 15: 1225-1233.
    2. Aguilar-Gonzalez A.E., Palou E., Lopez-Malo A. (2015): Antifungal activity of essential oils of clove (Syzygium aromaticus) and/or mustard (Brassica nigra) in vapor phase against grey mould (Botrytis cinerea) in strawberries. Innovative Food Science and Emerging Technologies, 32: 181-185. Go to original source...
    3. Aktaruzzaman M., Afroz T., Hong S.J., Kim B.S. (2017): Identification of Botrytis cinerea, the cause of post-harvest grey mould on broccoli in Korea. Research in Plant Disease, 23: 372-378. Go to original source...
    4. Ally N.M., Neetoo H., Ranghoo-Sanmukhiya M., Hardovar S., Vally V., Gungosingh-Bunwaree A., Maudarbaccus F., Coutinbo T.A., et al. (2021): First report of Botrytis cinerea causing mould on greenhouse-grown tomato plants in Mauritius. Plant Disease, 105: 2725. Go to original source... Go to PubMed...
    5. Azevedo D.M.Q., Guterres D.C., Martins S.D.S., Oliveira W.M., Barreto R.W., Furtado G.Q. (2020): First report of Botrytis cinerea causing grey mould on Sanchezia nobilis in Brazil. Plant Disease, 104: 3080. Go to original source...
    6. Bahmid N.A., Pepping L., Dekker M., Fogliano V., Heising J. (2020): Using particle size and fat content to control the release of allyl isothiocyanate from ground mustard seeds for its application in antimicrobial packaging. Food Chemistry, 308: 125573. Go to original source... Go to PubMed...
    7. Barea-Ramos J.D., Rodriguez M.J., Calvo P., Melendez F., Lozano J., Martin-Vertedor D. (2024): Inhibition of Botrytis cinerea in tomatoes by allyl-isothiocyanate release from black mustard (Brassica nigra) seeds and detection by E-nose. Food Chemistry, 432: 137222. Go to original source... Go to PubMed...
    8. Beck A.M., Marsh T.L. (2015): Inhibition of the general soil microbial community by allyl-isothiocyanate and benzyl-isothiocyanate. BIOS, 86: 31-37. Go to original source...
    9. Ben Ammar H., Arena D., Treccarichi S., Di Bella M.C., Marghali S., Ficcadenti N., Lo Scalzo R., Branca F. (2023): The effect of water stress on the glucosinolate content and profile: A comparative study on roots and leaves of Brassica oleracea L. crops. Agronomy, 13: 579. Go to original source...
    10. Bi K., Liang Y., Mengiste T., Sharon A. (2023): Killing softly: a roadmap of Botrytis cinerea pathogenicity. Trend in Plant Science, 28: 211-222. Go to original source... Go to PubMed...
    11. Bohinc T., Trdan S. (2012): Environmental factors affecting the glucosinolate content in Brassicaceae. Journal of Food, Agriculture & Environment, 10: 357-360.
    12. Chen Y., Xing M., Chen T., Tian S., Li B. (2023): Effects and mechanisms of plant bioactive compounds in preventing fungal spoilage and mycotoxin contamination in post-harvest fruits: A review. Food Chemistry, 415: 125787. Go to original source... Go to PubMed...
    13. Dai R., Lim L.T. (2014): Release of allyl isothiocyanate from mustard seed meal powder. Journal of Food Science, Go to original source... Go to PubMed...
    14. 79: E47-E53.
    15. Dubey S., Guignard F., Pellaud S., Pedrazzetti M., Schuren A., Gaume A., Schnee S., Gindro K., et al. (2021): Isothiocyanate derivates of glucosinolates as efficient natural fungicides. PhytoFrontiers, 1: 40-50. Go to original source...
    16. Fontana D.C., Neto D.D., Pretto M.M., Mariotto A.B., Caron B.O., Kulczynski S.M., Schmidt D. (2021): Using essential oils to control diseases in strawberries and peaches. International Journal of Food Microbiology, 338: 108980. Go to original source... Go to PubMed...
    17. Garfinkel A.R. (2021): The history of Botrytis taxonomy, the rise of phylogenetics, and implications for species recognition. Phytopathology, 111: 437-454. Go to original source... Go to PubMed...
    18. Hua L., Yong Ch., Zhanquan Z., Boqiang L., Guozheng Q., Shiping T. (2018): Pathogenic mechanisms and control strategies of Botrytis cinerea post-harvest decay in fruits and vegetables. Food Quality and Safety, 3: 111-119. Go to original source...
    19. Ismagulova A., Spanbayev A.D., Tulegenova Z., Eken C. (2021): First report of preharvest fruit rot of strawberry caused by Botrytis cinerea in Kazakhstan. Plant Disease, 105: 213. Go to original source... Go to PubMed...
    20. ISO 13299 (2016): Sensory Analysis - Methodology - General Guidance for Establishing a Sensory Profile. https://www.iso.org/standard/58042.html
    21. Jung Y.J., Padmanabahn A., Hong J.H., Lim J., Kim K.O. (2012): Consumer freshness perception of spinach samples exposed to different storage conditions. Post-harvest Biology and Technology, 73: 115-121. Go to original source...
    22. Kowalska B., Smolińska U. (2008): The effect of selected plant materials and extracts on the development of bacterial diseases on onion. Vegetable Crops Research Bulletin, 68: 33-45. Go to original source...
    23. Lietzow J. (2021): Biologically active compounds in mustard seeds: A toxicological perspective. Foods, 10: 2089. Go to original source... Go to PubMed...
    24. Neugart S., Baldermann S., Hanschen F.S., Klopsch R., Wiesner-Reinhold M., Schreiner M. (2018): The intrinsic quality of brassicaceous vegetables: How secondary plant metabolites are affected by genetic, environmental, and agronomic factors. Scientia Horticulturae, 233: 460-478. Go to original source...
    25. Pathak V.M., Verma V.K., Rawat B.S., Kaur B., Babu N., Sharma A., Dewali S., Yadav M., et al. (2022): Current status of pesticide effects on environmental, human health and it's eco-friendly management as bioremediation: A comprehensive review. Frontiers in Microbiology, 13: 962619. Go to original source... Go to PubMed...
    26. Plaszkό T., Szücs Z., Vasas G., Gonda S. (2021): Effects of glucosinolate-derived isothiocyanates on fungi: a comprehensive review on direct effects, mechanisms, structure-activity relationship data and possible agricultural applications. Journal of Fungi, 7: 539. Go to original source... Go to PubMed...
    27. PN-ISO 8589 (2007): Sensory Analysis - General Guidelines for Designing A Laboratory for Sensory Analysis. https://www.iso.org/standard/36385.html
    28. Poveda J., Eugui D., Velasco P. (2020): Natural control of plant pathogens through glucosinolates: an effective strategy against fungi and oomycetes. Phytochemical Reviews, 19: 1045-1059. Go to original source...
    29. Rani L., Thapa K., Kanojia N., Sharma N., Singh S., Grewal A.S., Srivastav A.L., Kaushal J. (2021): An extensive review on the consequences of chemical pesticides on human health and environment. Journal of Cleaner Production, 283: 124657. Go to original source...
    30. Redondo-Blanco S., Fernandez J., Lopez-Ibanez S., Miguelez E.M., Villar C.J., Lombo F. (2020): Plant phytochemicals in food preservation: antifungal bioactivity: a review. Journal of Food Protection, 83: 163-171. Go to original source... Go to PubMed...
    31. Shao W., Zhao Y., Ma Z. (2021): Advances in understanding fungicide resistance in Botrytis cinerea in China. Phytopathology, 111: 455-463. Go to original source... Go to PubMed...
    32. Sikorska A., Gugała M., Zarzecka K., Domański Ł. (2023): Effect of organic and inorganic stimulants on the content of glucosinolates in winter rape seed. Journal of Elementology, 28: 189-198. Go to original source...
    33. Sikorska-Zimny K., Beneduce L. (2021): The glucosinolates and their bioactive derivatives in Brassica: a review on classification, biosynthesis and content in plant tissues, fate during and after processing, effect on the human organism and interaction with the gut microbiota. Critical Reviews in Food Science and Nutrition, 61: 2544-2571. Go to original source... Go to PubMed...
    34. Sun Y., Wang Y., Xu Y., Chen T., Li B., Zhang Z., Tian S. (2021): Application and mechanism of benzyl-isothiocyanate, a natural antimicrobial agent from cruciferous vegetables, in controlling post-harvest decay of strawberry. Post-harvest Biology and Technology, 180: 111604. Go to original source...
    35. Tian Y., Deng F. (2020): Phytochemistry and biological activity of mustard (Brassica juncea): a review. CyTA - Journal of Food, 18: 704-718. Go to original source...
    36. Tian Y., Yang Z., Song W., Zhao H., Ye Q., Xu H., Hu B., Shen D., et al. (2023): Biofumigation by mustard plants as an application for controlling post-harvest grey mould in apple fruits. Agronomy, 13: 1490. Go to original source...
    37. Ugolini L., Martini C., Lazzeri L., D'Avino L., Mari M. (2014): Control of post-harvest grey mould (Botrytis cinerea Per.: Fr.) on strawberries by glucosinolate-derived allyl-isothiocyanate treatments. Post-harvest Biology and Technology, 90: 34-39. Go to original source...
    38. Wood E.M., Miles T.D., Wharton P.S. (2013): The use of natural plant volatile compounds for the control of the potato post-harvest diseases, black dot, silver scurf and soft rot. Biological Control, 64: 152-159. Go to original source...
    39. Yang B., Geng H., Zhang Ch., Wang G., Yang S., Gao S., Zhao Y., Xing F. (2021): Inhibitory effect of allyl and benzyl isothiocyanates on ochratoxin a producing fungi in grape and maise. Food Microbiology, 100: 103865. Go to original source... Go to PubMed...
    40. Zhu B., Liang Z., Zang Y., Zhu Z., Yang J. (2023): Diversity of glucosinolates among common Brassicaceae vegetables in China. Horticultural Plant Journal, 9: 365-380. Go to original source...
    41. Ziedan E.H. (2022): A review of the efficacy of biofumigation agents in the control of soil-borne plant diseases. Journal of Plant Protection Research, 62: 1-11.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content