Plant Protection Science, 2001 (vol. 37), issue 2

Transmission of 16SrV phytoplasmas by Scaphoi-deus titanus Ball in northern ItalyOriginal Paper

Piero Attilio Bianco, Alberto Alma, Paola Casati, Giorgo Scattini, Alessandra Arzone

Plant Protect. Sci., 2001, 37(2):49-56 | DOI: 10.17221/8365-PPS  

Flavescence dorée (FD) has been defined as the disease that is caused by a phytoplasmas belonging to group 16SrV (elm yellows) and transmitted to plants by the insect Scaphoideus titanus Ball. We investigated transmission of FD agent by S. titanus in a vineyard located in Veneto region to determine which phytoplasma(s) may be transmitted in this region. Group 16SrV-C phytoplasma was detected and identified in field-collected S. titanus adults, in plant of grapevine (Vitis vinifera L. cv. Gamay) and broadbean (Vicia faba cv. Arlŕ) that had been fed upon by the insects. No evidence of experimental transmission...

Utilization of immunochemical methods to detect Phytophthora fraga-riae - a quarantine pathogen of strawberryOriginal Paper

Blanka Pekárová, Jiřina Krátká, Jakub Slováček

Plant Protect. Sci., 2001, 37(2):57-65 | DOI: 10.17221/8366-PPS  

Polyclonal antibodies anti-PfP IgG and anti-PfM IgG and monoclonal antibody MAb29 were prepared to detect a quarantine pathogen of strawberry, Phytophthora fragariae. Laboratory rabbits and mice were immunized using purified and unpurified protein extracts from the mycelial mass of the pathogen. All prepared antibodies were genus-specific, therefore Phytophthora cactorum was also detected. Except for Pythium ultimum, the antibodies did not cross-react with other pathogenic fungi, such as Botrytis cinerea, Colletotrichum acutatum, Fusarium sp., Verticillium albo-atrum. PTA-ELISA was used to test...

Reaction of winter wheat varieties to Fusarium graminearum and F. culmorum in field infection trials and the efficacy of fungicidesOriginal Paper

Marie Váňová, Ludvík Tvarůžek, Hana Hrabalová

Plant Protect. Sci., 2001, 37(2):66-73 | DOI: 10.17221/8367-PPS  

Fifteen winter wheat varieties were tested for their reaction to Fusarium culmorum and F. graminearum in the ear in 1996, 1998 and 1999. To ensure a sufficient level of infection in the field the varieties were artificially inoculated with spores of the two species at a ratio of 1:1. The infection was evaluated using a 10-score scale at three times: first at the milk stage (BBCH 71-75), i.e. about 14 days after inoculation; two further readings were taken at 7-day intervals. The data were used to calculate the average infection for each variety at each reading, and were statistically evaluated. The parameter "area under disease progress...

Evaluation of resistance to Fusarium oxysporum f.sp. sesami in hybrid lines of sesame (Sesamum indicum L.) under greenhouse conditionsOriginal Paper

M. A. El-Bramawy, Karel Veverka, Slavoj Vaverka, M. S. El-Shazly, M. A. El-Sattar, M. A. El-Ashary, S. E. Ammar

Plant Protect. Sci., 2001, 37(2):74-79 | DOI: 10.17221/8368-PPS  

Thirty-six samples of sesame (15 F1 and 15 F2 generations and their 6 parents) were evaluated for their reaction to Fusarium oxysporum f.sp. sesami (Zaprometoff) Castellani after artificial inoculation in greenhouse tests. The experimental soil was analysed mechanically and microbiologically. Reactions of the plants were recorded on a scale from 0 = no visible infection (immune) up to 6 = 70% infected plants (highly susceptible). Highly significant differences of susceptibility and resistance to the wilt pathogen were observed among hybrids and their parents. The level of infection in the parental generation varied from 2.22 to 63.77%...