Plant Protection Science, 2002 (vol. 38), issue 2
Determination of the cluster of wheat rust resistance genes Yr17, Lr37, and Sr38 by a molecular marker
Monika Ambrozková, Francoise Dedryver, Veronika Dumalasová, Alena Hanzalová, Pavel Bartoš
Plant Protect. Sci., 2002, 38(2):41-45 | DOI: 10.17221/4849-PPS 
A translocation from Aegilops ventricosa carrying genes Yr17, Lr37 and Sr38 was verified in cultivars Hussar, Eureka, Torfrida, Renan, Rendezvous, Rapier and Brigadier by the molecular marker SCARSC-Y15. Of the cultivars recently registered in the Czech Republic, only the western European cultivars Corsaire, Apache, Complet and Bill possessed the translocation carrying Yr17, Lr37 and Sr38. Cultivars Corsaire, Apache, Bill and Complet were highly or medium resistant to yellow rust and leaf rust in 1999-2001 field trials at Prague-Ruzyně, although virulence to Yr17...
Damage to corn by fungi of the genus Fusarium and the presence of fusariotoxins
Jan Nedělník
Plant Protect. Sci., 2002, 38(2):46-54 | DOI: 10.17221/4848-PPS
In 1998 and 1999 a total of 84 samples of corn, predominantly from localities in southern and central Moravia, were collected either directly from fields (entire ears at harvest maturity) or as grain from merchants. The objectives of the experiments were (a) to determine, on the basis of the results from mycological and toxicological analyses, the basic spectrum of fungal contaminants of corn in the Czech Republic with special reference to the genus Fusarium, and (b) to determine by enzyme immunoassay the presence of major toxic metabolites such as deoxynivalenol (DON), T-2 toxin (T-2), zearalenone (ZEA), and fumonisins (FUM) in grain samples....
Utilisation of immunochemical methods for detection of Colletotrichum spp. in strawberry
Jiřina Krátká, Blanka Pekárová-Kyněrová, Iva Kudlíková, Jakub Slováček, Michaela Zemánková
Plant Protect. Sci., 2002, 38(2):55-63 | DOI: 10.17221/4850-PPS
Four polyclonal and two monoclonal antibodies were prepared and tested to detect a quarantine pathogen of strawberry - Colletotrichum acutatum. Only one of them, polyclonal IgG K91, was sensitive enough to recognize the pathogen. This antibody was genus-specific and did not cross-react with several other fungal pathogens of strawberry (Phytophthora fragariae, P. cactorum, Verticillium albo-atrum, Botrytis cinerea, Pythium ultimum). Four techniques, PTA-ELISA, dot blot, immunoprint and immunofluorescent microscopy were used to test the specifity and sensitivity of antibodies. After artificial infection of strawberry (cvs Elsanta, Vanda,...
Identification of genes for resistanceto wheat powdery mildew in Hungarian, Polish and Slovak wheat cultivars
Miroslav Švec, László Szunics, Marta Miklovičová, Tatiana Slováková, Viera Tisová, Pavol Hauptvogel
Plant Protect. Sci., 2002, 38(2):64-72 | DOI: 10.17221/4851-PPS
The objective of the study was to identify genes for resistance to powdery mildew in wheat cultivars and land races from Poland, Slovakia and Hungary. The presence and distribution of resistance genes was compared to powdery mildew virulence structure in these countries. The different strategies in breeding for resistance were expressed in a different distribution of resistance genes, especially among Polish and Hungarian cultivars. In 20 of the 29 Hungarian common wheat cultivars the resistance gene Pm8 was found. Of the 32 Polish cultivars investigated, 16 possess a combination of genes Pm2+6. Resistance gene Pm4b did not...
Field resistance of six cultivars of winter oilseed rape against Turnip yellow mosaic virus
Josef Špak, Darina Kubelková
Plant Protect. Sci., 2002, 38(2):73-75 | DOI: 10.17221/4852-PPS
The resistance of six cultivars of winter oilseed rape (SL 509, SL 507, Darmor, Solida, Jet Neuf, Silesia) against Turnip yellow mosaic virus was studied. The number of over-wintering plants and plants with symptoms of TYMV infection were monitored. All plants were tested by the double diffusion test in agar and by DAS-ELISA to prove infection. ELISA was the most sensitive method, revealing 32-76% of latent virus infections in individual cultivars. The results clearly illustrated that monitoring of plant symptoms is not sufficient to prove TYMV infection in the field and that sensitive, large scale methods like ELISA must be employed to obtain...
Thermal requirements for flight of six species of flea beetle of the genus Phyllotreta (Coleoptera: Chrysomelidae)
František Kocourek, Pavel Láska, Vojtěch Jarošík
Plant Protect. Sci., 2002, 38(2):76-80 | DOI: 10.17221/4865-PPS
The flight activity of six species of the flea beetles of the genus Phyllotreta was monitored by means of yellow water traps in three years. The percentage composition of the total catch of adults made up of the species was 29.5% (P. vittula), 28.8% (P. atra), 17.5% (P. undulata), 12.8% (P. nigripes), 7.1% (P. vittata) and 4.3% (P. nemorum). The flight activity of each species had two peaks. The spring peak was made up of overwintering adults migrating to host plants, and the summer peak of newly emerged adults. A regression model of flight activity in both generations was established based...
Alternaria alternata (Fr.) Keissler - New pathogen on sugar beet leaf in Slovakia - New and Unusually Reports
Kamil Hudec, Tibor Roháčik
Plant Protect. Sci., 2002, 38(2):81-82 | DOI: 10.17221/4854-PPS