Plant Protection Science, 2005 (vol. 41), issue 4
Index of Volume 41Index
editors
Plant Protect. Sci., 2005, 41(4):I-VI | DOI: 10.17221/2753-PPS 
Interference in PCR: Transcription amplifications of mixed PVY isolates
Arie Rosner, Ludmila Maslenin
Plant Protect. Sci., 2005, 41(4):125-131 | DOI: 10.17221/2750-PPS
Interference in PCR and transcription amplifications by mixed virus templates is described. The yields of PCR amplification of closely related PVY isolates in a mixture were lower than those of the separate amplification of each individual virus strain, i.e. the two virus templates mutually interfered in their amplification process. When PCR products of mixed PVY isolates served as templates for transcription, RNA synthesis was also inhibited. This interference could be avoided by applying strain-specific primers in the separate amplification of each target sequence in the mixture. The use of strain-specific primers in PCR amplification of a mixed...
Detection of phytoplasma ESFY in apricot trees using phloem and petioles
Tomáš Nečas, Boris Krška
Plant Protect. Sci., 2005, 41(4):132-140 | DOI: 10.17221/2751-PPS
ESFY phytoplasma (European stone fruit yellows phytoplasma) is nowadays one of the most important plant diseases, especially on apricots and peaches, and it belongs to the list of organisms for which quarantine is required in the Czech Republic. The aim of this study was to determine the best period for tissue extraction and the best technique for ESFY detection. It was also to investigate the possibility of isolating DNA for use in ESFY detection from the leaf-stalks of randomly chosen symptomatic and asymptomatic apricot trees. Results of the amplification of DNA extracted from leaf-stalk and phloem sampled from 2-year old woody shoots during the...
PCR detection of Pseudoperonospora humuli and Podosphaera macularis in Humulus lupulus
Josef Patzak
Plant Protect. Sci., 2005, 41(4):141-149 | DOI: 10.17221/2749-PPS
Hop downy mildew (Pseudoperonospora humuli) and hop powdery mildew (Podosphaera macularis) are the most important pathogens of hop (Humulus lupulus). The early detection and identification of these pathogens are often made difficult by symptomless or combined infection with another pathogens. Molecular analysis of internal transcribed spacer (ITS) regions of rDNA is a novel and very effective method of species determination. Therefore, specific PCR assays were developed to detect the pathogens Pseudoperonospora humuli and Podosphaera macularis in naturally infected hop plants. The specific PCR primer...
Harmful occurrence of Rosy rustic moth (Hydraecia micacea) (Noctuidae: Lepidoptera) on hop in the Czech Republic
Josef Šedivý, Petr Born, Josef Vostřel
Plant Protect. Sci., 2005, 41(4):150-157 | DOI: 10.17221/2748-PPS
The Rosy rustic moth (Hydraecia micacea) has been a pest of hop in Czech hop regions for a long time. It causes most severe damages in hop gardens infested by quackgrass (Elytrigia repens) on waterlogged areas. Larvae emerge from the second half of April to the middle of May. The sum of effective temperatures (SET) necessary for hatching larvae has been determined to be 78.6C over a 4-year average. Young larvae first feed on leaves of quackgrass, and later move to hop plants where they feed inside the shoots and rootstocks. Occurrence and damage are most frequent at the edges of hop gardens and in places with anchorages between two...
Pest status of the leafhopper Empoasca dolichi Paoli on groundnut (Arachis hypogaea L.) in the Zaria area of northern Nigeria
Elizabeth Agbenu Egwurube, Michael Ogunleke Ogunlana, Michael Chidozie Dike, Isa Onu
Plant Protect. Sci., 2005, 41(4):158-164 | DOI: 10.17221/2754-PPS
Population studies and tests on the relationship between density and damage were conducted in 1999 to 2001 to determine the pest status of Empoasca dolichi on groundnut (Arachis hypogaea L.) in the Zaria area of northern Nigeria. Analyses showed that Empoasca numbers varied significantly from one year to another, and within each year the numbers of leafhoppers observed at the different growth stages of the plant were significantly different (P = 0.01). There was an inverse and highly significant relationship between the mean kernel yield and the Empoasca damage at the different growth stages of the plant....
Destruction of chlorophyll in emerging seedlings of spring barley associated with environmental stresses
Václav Kůdela, Bohumila Voženílková, Radka Krejzarová, Václav Krejzar, Michal Janouch
Plant Protect. Sci., 2005, 41(4):165-170 | DOI: 10.17221/2752-PPS
An unusual disorder occurred on spring barley seedlings in southern and central Bohemia in April 2005. Affected seedlings showed conspicuous bleached or straw-coloured areas on tips of the first leaves. There were very sharp, distinct, horizontal boundaries between the green basal and discoloured tip parts of the leaves. It was remarkable that these boundaries were at the same position on all affected first leaves of seedlings from the same field. Affected seedlings were randomly distributed in the fields, and their incidence ranged from about 20 to 70% according to location. Based on meteorological data it can be concluded that the colour abnormality...