Plant Protection Science, 2006 (vol. 42), issue 2
Occurrence and distribution of mating types A1 and A2 of Phytophthora infestans (Mont.) de Bary in the Czech Republic
Jana Mazáková, Vladimír Táborský, Miloslav Zouhar, Pavel Ryšánek, Ervín Hausvater, Petr Doležal
Plant Protect. Sci., 2006, 42(2):41-48 | DOI: 10.17221/2697-PPS 
A total of 199 Phytophthora infestans isolates were obtained from leaves, tubers and fruits of infected crops of potato and tomato in different regions of the Czech Republic in 2003, 2004 and 2005. They were analysed for mating type using the conventional pairing assay and PCR markers; 107 isolates were of A1 and 92 of A2 mating type. No self-fertile isolate was found. Our study is the first report of the presence and distribution of the A2 mating type of P. infestans in the Czech Republic. The co-existence of the two mating types may enable the pathogen to reproduce sexually, thus enhancing the diversity of its population countrywide.
Rapid detection of Ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and PCR
Muthusamy Karthikeyan, Krishnan Radhika, Ramanujam Bhaskaran, Subramanian Mathiyazhagan, Ramasamy Samiyappan, Rethinasamy Velazhahan
Plant Protect. Sci., 2006, 42(2):49-57 | DOI: 10.17221/2771-PPS
Molecular and immunological methods were applied for detecting the Ganoderma disease of coconut. Polyclonal antibodies (PAbs) raised against basidiocarp protein of Ganoderma were used. For the polymerase chain reaction (PCR) tests, the primer generated from the internal transcribed spacer region one (ITS 1) of ribosomal DNA gene of Ganoderma, which produced a PCR product of 167 bp in size, was used. Apparently healthy palms in two coconut gardens were tested for Ganoderma disease by ELISA test using basidiocarp protein antiserum. Field trials were laid out in these early-diagnosed palms for the management of the...
Occurrence of biotic harmful agents in Czech grass seed production (1995-2004)
Bohumír Cagaš, Jan Macháč, Jan Frydrych, Radek Macháč
Plant Protect. Sci., 2006, 42(2):58-65 | DOI: 10.17221/2695-PPS
In the years 1995-2004 the spectra of weeds, diseases and pests in grasses grown for seed in the Czech Republic were examined and the effect of external factors on their changes was assessed. The species spectrum of weeds evaluated both at the time of vegetation and in samples of natural seeds was stable throughout the period and the changes in the number of individual species were caused by different proportions of grass species and stand age. The most abundant weeds were Elytrigia repens, Matricaria sp.div., Anthemis sp.div., Apera spica-venti, Cirsium arvense, Poa trivialis, Rumex sp.div....
Field resistance of crosses of sesame (Sesamum indicum L.) to charcoal root rot caused by Macrophomina phaseolina (Tassi.) Goid.
Mohamed A. S. El-Bramawy, Omar A. Abdul Wahid
Plant Protect. Sci., 2006, 42(2):66-72 | DOI: 10.17221/2773-PPS
Two segregating generations (F3 and F4) from 6 × 6 half-diallel crosses, excluding reciprocals, of a sesame breeding program were exposed to natural infection by the root rot pathogen (Macrophomina phaseolina) in two successive seasons (2004 and 2005). There was highly significant variability in the progeny of all investigated crosses which might be a valuable tool for further breeding programs for root rot disease management. The level of infection in 2004 ranged from 2.63% (cross P2 × P5) to 52.42% (P4 × P5) in the F3, and from 1.28% (P1 × P5)...
Population dynamics of Chaetocnema tibialis Illiger and Phyllotreta vittula (Redtenbacher) on the weed Amaranthus retroflexus L. and cultivated Amaranthus caudatus L.
Ľudovít Cagáň, Peter Tóth, Monika Tóthová
Plant Protect. Sci., 2006, 42(2):72-80 | DOI: 10.17221/2696-PPS
In 1995-1997, the population dynamics of the flea beetles Chaetocnema tibialis and Phyllotreta vittula, associated with Amaranthus retroflexus (wild species) and Amaranthus caudatus (cultivated species), were studied at the locality Nitra-Malanta (48°19'N, 18°09'E) in south-western Slovakia. On both plant species, the number of C. tibialis adults was usually very low until the beginning of July. During July the number of C. tibialis increased, but sooner on cultivated amaranth. An increased number of C. tibialis adults was observed on both amaranth species until the middle of September. The...
Eyualem-Abebe, W. Traunspurger, I. Andrássy (eds):Freshwater Nematodes: Ecology and Taxonomy - Book Review E. Heuvelink (ed.): Tomatoes - Book Review
Aleš Lebeda
Plant Protect. Sci., 2006, 42(2):81-84 | DOI: 10.17221/2772-PPS