Plant Protection Science, 2010 (vol. 46), issue 3
Mating type distribution of Phytophthora infestans (Mont.) de Bary in the Czech Republic in 2007 and 2008
Jana Mazáková, Miloslav Zouhar, Pavel Ryšánek, Vladimír Táborský, Ervín hausvater, Petr Doležal
Plant Protect. Sci., 2010, 46(3):89-97 | DOI: 10.17221/49/2009-PPS 
A total of 195 isolates of Phytophthora infestans were collected from late blight-diseased potatoes grown in several localities in the Czech Republic during the years 2007-2008. The isolates were analysed for mating type using the pairing test, CAPS marker assays and PCR assays. Of the 195 tested isolates, 28% were of the A1 mating type and 75% corresponded to the A2 mating type. Furthermore, oospores of P. infestans were microscopically detected in leaf samples from one locality.
Diversity of oat crown rust (Puccinia coronata f.sp. avenae) isolates detected by virulence and AFLP analyses
Hana Klenová-Jiráková, Leona Leišová-Svobodová, Alena Hanzalová, Ladislav Kučera
Plant Protect. Sci., 2010, 46(3):98-106 | DOI: 10.17221/17/2009-PPS
Forty Puccinia coronata f.sp. avenae isolates from several European countries and Israel were tested for virulence to 18 differential oat lines. Amplified Fragment Length Polymorphism (AFLP) analysis was used to evaluate diversity among the studied isolates. Twenty-nine different pathotypes were identified. The prevailing occurrence of pathotypes with a limited number of virulence genes may indicate that oat cultivars grown in Europe possess the limited number of resistance genes. A total of 501 AFLP polymorphic fragments were scored in the studied isolates using twelve primer combinations. All isolates had the unique AFLP molecular...
Quantification of Tilletia caries and Tilletia controversa mycelium in wheat apical meristem by real-time PCR
Miloslav Zouhar, Jana Mazáková, Evženie Prokinová, Marie Váňová, Pavel Ryšánek
Plant Protect. Sci., 2010, 46(3):107-115 | DOI: 10.17221/50/2009-PPS
In the Czech Republic, three closely related species of the genus Tilletia belong to pathogens that cause significant losses of wheat crops by replacing grains with a mass of teliospores. A quantitative real-time qPCR assay using SYBR Green I has been developed to quantify the amount of T. caries and T. controversa mycelium in apical meristems of different wheat varieties. The real-time PCR reaction was validated by evaluating selected extraction methods, examining the specificity of designed target-specific IGS primers and verifying the optimised amplification reaction on naturally infected wheat plants. The PCR detection...
Field efficacy of Helicoverpa armigera nucleopolyhedro-virus isolates against H. armigera (Hübner) (Lepidoptera: Noctuidae) on cotton and chickpea in Tamil Nadu
Subramanian Jeyarani, Natarajan Sathiah, Palaniappan Karuppuchamy
Plant Protect. Sci., 2010, 46(3):116-122 | DOI: 10.17221/30/2009-PPS
Field efficacy of seven geographical isolates of Helicoverpa armigera nucleopolyhedrovirus (HaNPV) along with an insecticide control was evaluated against H. armigera on cotton and chickpea in the Coimbatore district of Tamil Nadu, India. Among the HaNPV isolates, CBE I (Coimbatore) and NEG (Negamum) applied at 3.0 × 1012 POB/ha to cotton and 1.5 × 1012 POB/ha to chickpea with an adjuvant, crude sugar, significantly reduced the H. armigera larval population and increased the yield. CBE I and NEG recorded the highest yield of 2038 kg/ha and 2033 kg/ha, which was on a par with endosulfan (2026.7 kg/ha)...
Biological efficacy of some biorational and conventional insecticides in the control of different stages of the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)
Mohamed A. M. Osman
Plant Protect. Sci., 2010, 46(3):123-134 | DOI: 10.17221/27/2009-PPS
The biological efficacy of some biorational and conventional insecticides against different stages of Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) was evaluated under laboratory and semi-field conditions. Seven different commercial products were tested, including the biorational insecticides: Spinosad, Mectin, Fitoverm, Match, Neemix in addition to two conventional insecticides: Actara and Actellic. Data indicated that all tested insecticides showed low toxic effects to L. decemlineata eggs, but most hatching neonates died shortly after hatching. All tested insecticides at their field rates showed...
Bifenazate, a prospective acaricide for spider mite (Tetranychus urticae Koch) control in Czech hops
Josef Vostřel
Plant Protect. Sci., 2010, 46(3):135-138 | DOI: 10.17221/54/2009-PPS
Bifenazate, a new selective carbazate acaricide, seems to be a very good substitute for propargite to control spider mites (Tetranychus urticae Koch) on hops in the Czech Republic. To investigate the phenomenon of T. urticae resistance to this compound, 20 samples of field populations were taken in several Czech and Moravian hop-growing regions in 2006 and 2007 and subjected to laboratory tests in a Potter tower. Low values of C100 M (100% mortality) in comparison with the supposed registered concentration reveal that bifenazate may become a useful acaricide within the anti-resistant strategy against T. urticae not only in...