Plant Protection Science, 2013 (vol. 49), issue 3

Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virusOriginal Paper

Helena KYSELÁKOVÁ, Michaela SEDLÁŘOVÁ, Martin KUBALA, Vladimíra NOŽKOVÁ, Jana PITERKOVÁ, Lenka LUHOVÁ, Ondřej NOVÁK, Petr ILÍK

Plant Protect. Sci., 2013, 49(3):105-119 | DOI: 10.17221/51/2012-PPS  

The physiological responses of pea plants during 40 days of compatible interaction with Pea enation mosaic virus (PEMV) were evaluated. PEMV induces systemic changes in the concentration of phytohormones. At 5 days post inoculation (dpi), a simultaneous increase in abscisic acid (ABA) level and a decrease in salicylic acid (SA) level was observed, which is known to be involved in the suppression of hypersensitive reaction. In our pathosystem it preceded the virus presence in the systemic leaves. PEMV induces the accumulation of ABA, SA and Hsp70 and enhances peroxidase (POX) activity at 15 dpi, when it is already transmitted throughout the...

Patterns and severity of crown dieback in young even-aged stands of european ash (Fraxinus excelsior L.) in relation to stand density, bud flushing phenotype, and seasonOriginal Paper

Remigijus BAKYS, Rimvydas VASAITIS, Jens Peter SKOVSGAARD

Plant Protect. Sci., 2013, 49(3):120-126 | DOI: 10.17221/70/2012-PPS  

The extent and temporal pattern of crown damage (attributed to Hymenoscyphus pseudoalbidus) in even-aged stands of Fraxinus excelsior in relation to bud flushing phenotype, stand density, and season was investigated. Data were collected in 2007 in four statistically designed thinning experiments located in 12-15-years old plantations of ash in Denmark. The study included 21 plots of four contrasting, residual stand densities: (1) 1700-5500 trees/ha (unthinned control plots), (2) 1500 trees/ha, (3) 500 trees/ha, and (4) 100-150 trees/ha. Assessments included estimation of flushing phenotype in May, followed by evaluation of severity...

Anastomosis grouping of Rhizoctonia solani and binucleate Rhizoctonia spp. isolated from pepper in Erzincan, TurkeyOriginal Paper

Serdar Tuncer, Cafer Eken

Plant Protect. Sci., 2013, 49(3):127-131 | DOI: 10.17221/77/2012-PPS  

Ninety eight isolates of Rhizoctonia spp. were obtained from roots of pepper (Capsicum annuum L.) grown in Erzincan, Turkey during the period 2007-2008. The most prevalent multinucleate anastomosis groups (AG) were AG-4 (85.2%), followed by AG-2 type 1 (7.4%), AG-6 (5.0%), and AG-3 (2.5%). The population of binucleate Rhizoctonia spp. comprised AG-A (82.4%), AG-K (11.8%), and AG-G (5.9%). Rhizoctonia solani AG-3 and AG-6, as well as binucleate Rhizoctonia spp. AG-G and AG-K on pepper (C. annuum) were firstly determined in this study. During both in vitro and in vivo pathogenicity...

Evaluation of safflower varieties for resistance to the fungal pathogen, Colletotrichum acutatumOriginal Paper

Jana Víchová, Barbora Staňková, Radovan Pokorný, Karel Vejražka

Plant Protect. Sci., 2013, 49(3):132-136 | DOI: 10.17221/23/2012-PPS  

The influence of plant growth stage, inoculum density, and variety on the disease severity and incidence were studied. Our results indicated that growth stage of the plant had no influence on infection rate, as the pathogen attacked 100% of tested plants. When applying three different inoculum concentrations to three weeks old safflower plants, no statistical differences were recorded, disease incidence ranged 32.0-47.0%. In this test, the 3rd and 4th evaluated leaves (the youngest) were infected more intensively than were the 1st and 2nd leaves. Among 11 safflower varieties tested for resistance to this...

Interactive effect of AM fungi with Trichoderma viride and Pseudomonas fluorescens on growth and yield of broccoliOriginal Paper

Anju Tanwar, Ashok Aggarwal, Sunita Kaushish, Sonika Chauhan

Plant Protect. Sci., 2013, 49(3):137-145 | DOI: 10.17221/54/2012-PPS  

Most of the vegetable crops are known to depend upon arbuscular mycorrhizal fungal (AM) symbiosis for growth and development, since AM fungi provide nutrients and water in exchange for photosynthates. The influences of AM fungi (Glomus intraradices (G) and Acaulospora laevis (A)) with Trichoderma viride (T) and Pseudomonas fluorescens (P) alone and in combinations on growth, mycorrhization, chlorophyll content, nutrient uptakes, and yield of broccoli plants were studied in pot culture under glasshouse conditions. The obtained results demonstrated that the single inoculation of broccoli plants with T. viride...

Diversity of carabid beetles (Coleoptera: Carabidae) under three different control strategies against European corn borer in maizeOriginal Paper

František Kocourek, Pavel Saska, Milan Řezáč

Plant Protect. Sci., 2013, 49(3):146-153 | DOI: 10.17221/78/2012-PPS  

We compared three control strategies against European corn borer (Ostrinia nubilalis Hubner) in maize with respect to carabid beetles, beneficial epigeal arthropods. The impact of the focal treatment (insect resistant Bt maize MON 810) was compared with conventionally farmed and Trichogramma-treated plots at two sites (Prague-Ruzyně and Ivanovice na Hané) in the Czech Republic, replicated in three cropping seasons (2002-2004). The sampled assemblages were species-poor. The species were unevenly distributed in terms of their catch size - the communities were dominated by 7 (Ruzyně) or 3 (Ivanovice) species. No differences were found...