Plant Protection Science, 2018 (vol. 54), issue 3
Effect of plant growth-promoting bacteria Bacillus amylliquefaciens Y1 on soil properties, pepper seedling growth, rhizosphere bacterial flora and soil enzymesOriginal Paper
Qaiser Jamal, Yong Seong Lee, Hyeon Deok Jeon, Kil Young Kim
Plant Protect. Sci., 2018, 54(3):129-137 | DOI: 10.17221/154/2016-PPS 
The Bacillus amyloliquefaciens Y1 strain was evaluated for its effects on soil properties, pepper seedling growth, rhizosphere bacterial flora and soil enzyme activities. Y1 solubilised insoluble phosphate, produced chitinase, and released siderophores in plate detection assay. In order to evaluate the plant growth promotion potential in vivo, strain Y1 was grown in media containing chitin powder and complex fertiliser. The pot experiment was conducted by treating pepper seedlings with C1/1 (Y1 culture, 50 ml), C2/3 (Y1 culture, 33 ml), C1/2 (Y1 culture, 25 ml), F1/1 (complex fertiliser, 50 ml), F1/2 (complex fertiliser, 25 ml), and...
Biological control of watermelon seedling blight caused by Acidovorax citrulli using antagonistic bacteria from the genera Curtobacterium, Microbacterium and PseudomonasOriginal Paper
Sumer Horuz, Yesim Aysan
Plant Protect. Sci., 2018, 54(3):138-146 | DOI: 10.17221/168/2016-PPS
The biological control of the watermelon seedling blight and fruit blotch disease was investigated by screening the potential use of antagonistic bacteria. Between May and August 2012, totally 322 putative antagonistic bacteria were isolated from symptomless melon and watermelon plants grown in Adana, Hatay, and Osmaniye provinces of the Eastern Mediterranean Region of Turkey. In vitro dual culture tests showed that 54 out of 322 strains inhibited the Acidovorax citrulli (Ac) growth with an appearance of clear zones between 2.3 and 27.0 mm in diameter. However, the remaining 268 strains did not exhibit any antagonistic...
Molecular diversity and assessment of reactions of pepper pure line germplasm to Botrytis cinereaOriginal Paper
Ilknur Polat, Ömür Baysal, Emine Gümrükcü, Görkem Sülü, Aytül Kitapci, Ramazan Özalp, Ibrahim Çelik, Zübeyir Devran, Ersin Polat
Plant Protect. Sci., 2018, 54(3):147-152 | DOI: 10.17221/44/2017-PPS
The host resistance level of pure line materials was assessed in the genepool for the purpose of breeding. The highest resistance to the pathogen was observed in bell-type pepper. Moreover, genetic diversity of pure lines was investigated using selected inter-simple sequence repeat (ISSR) primers. Generally, genetic markers showed genetic diversity, so that long-type pure lines were separated from the other accessions. This is the first report on host reactions of Turkish pure lines as breeding material. These results provide significant information for future pepper breeding programs.
In vitro evaluation of eight plant essential oils for controlling Colletotrichum, Botryosphaeria, Fusarium and Phytophthora fruit rots of avocado, mango and papayaOriginal Paper
Ali Sarkhosh, Bruce Schaffer, Ana I. Vargas, Aaron J. Palmateer, Patricia Lopez, Asiye Soleymani
Plant Protect. Sci., 2018, 54(3):153-162 | DOI: 10.17221/49/2017-PPS
In vitro efficacy of the essential oils extracted from eight plant species was tested at application rates of 100, 250, 500, 1000, or 2000 µl/l for controlling fruit rots. Results showed a 100% reduction of mycelium growth of Colletotrichum, Fusarium, Phytophthora, Botryosphaeria after applying thyme or savory oils at all concentrations tested. Mint and cinnamon oils inhibited mycelium growth of the five isolates at application rates of either 1000 or 2000 µl/l. Tea tree, lavender, myrtle, and eucalyptus oils were slightly effective at controlling mycelium growth of each fungus species tested....
Effectiveness of environmentally safe food additives and food supplements in an in vitro growth inhibition of significant Fusarium, Aspergillus and Penicillium speciesOriginal Paper
Martin ®abka, Roman Pavela
Plant Protect. Sci., 2018, 54(3):163-173 | DOI: 10.17221/86/2017-PPS
We tested 38 legislatively recognised substances such as food additives and supplements for antifungal efficacy, with the aim of providing an alternative to synthetic conventional fungicides. These compounds were tested against 9 significant pathogenic fungal species belonging to the significant genera Fusarium, Penicillium, and Aspergillus. Of these compounds, 6 are proposed as potential candidates to provide a complementary alternative to conventional fungicides. Natamycin provided extreme efficacy expressed as MIC50 (5-31 µg/ml), followed by BHA and then BHT, CaNa2EDTA, PABA, and chitosan expressed as MIC50 (0.7-1.9 mg/ml). Safety and antifungal...
Assessment of the influence of fertilisation and environmental conditions on maize healthOriginal Paper
Piotr Szulc, Jan Bocianowski, Kamila Nowosad, Tadeusz Michalski, Hubert Waligóra, Pawe³ Olejarski
Plant Protect. Sci., 2018, 54(3):174-182 | DOI: 10.17221/22/2017-PPS
Field experiments showed the occurrence of such agrophages as the frit fly (Oscinella frit L.) and the European maize borer (Pyrausta nubilalis Hbn.). Identified diseases included fusariosis (Fusarium ssp.) and maize smut (Ustilago maydis Corda). The incidence of the frit fly was influenced by weather conditions, mainly temperature, in the period from sowing to the BBCH 15-16 phase. Moderate temperature conditions contributed to the occurrence of the pest. The significantly highest percentage of plants damaged by larvae of this pest was recorded on maize fertilised only with potassium (K) and phosphorus...
Distribution and molecular identification of Meloidogyne spp. parasitising flue-cured tobacco in Yunnan, ChinaOriginal Paper
Jianmin Zeng, Zhe Zhang, Meiyun Li, Xingfu Wu, Yongsan Zeng, Yongping Li
Plant Protect. Sci., 2018, 54(3):183-189 | DOI: 10.17221/82/2017-PPS
Twenty-one populations of root-knot nematodes (RKNs) were recovered from diseased roots collected from flue-cured tobacco in 21 locations in Yunnan (China) during 2014-2015. Molecular diagnosis on species was performed based on characteristics of sequences of D2-D3 expansion domains of the 28S rDNA and sequence characterised amplified regions (SCAR). SCAR results identified 13 populations as Meloidogyne arenaria, six as M. javanica and two as M. incognita. Meloidogyne arenaria is the predominant species with a detection rate of 61.9%, followed by M. javanica (28.5%) and M. incognita (9.5%). The...
Response of mated insects of both sexes of granary weevil to blends of volatiles - short communicationShort Communication
Anna Wenda-Piesik, Dariusz Piesik, Anita Krasiñska
Plant Protect. Sci., 2018, 54(3):190-193 | DOI: 10.17221/136/2017-PPS
We report the behavioural responses of mated granary weevils to blends of cereal volatiles. Four doses were applied on filter paper (1, 10, 100, and 1000 ng/min in 50 µl of hexane applied on filter paper). A Y-tube experiment revealed that mated females of Sitophilus granarius were attracted to blend 1, 4, and 5 at concentrations of 1000, 100, 1, and 10 ng/min, respectively. Mated males were attracted only to blend 5 at a concentration of 10 ng/minute. Yet, the mated weevil females and males were repelled by the highest concentration (1000 ng/min) for all tested blends. Moreover, both mated sexes were repelled by 100 ng/min in blends...
Using leaf chlorophyll fluorescence for in-season diagnosing herbicide resistance in Echinochloa species at reproductive growth stageOriginal Paper
Chuan-Jie Zhang, Do-Soon Kim
Plant Protect. Sci., 2018, 54(3):194-202 | DOI: 10.17221/51/2017-PPS
The chlorophyll fluorescence measurement to diagnose herbicide resistant Echinochloa species at the reproductive (late) growth stage was applied. The significant correlation between Fv/Fm (chlorophyll fluorescence measurements) and fresh weight (whole plant test) and the statistical similarity of R/S ratios between the two tests demonstrated that the chlorophyll fluorescence test could be reliably used to diagnose herbicide resistant Echinochloa spp. at the reproductive growth stage in a shorter period of time (within 10 days) compared with the conventional whole plant test.