Plant Protect. Sci., 1999, 35(2):41-46 | DOI: 10.17221/9673-PPS

Preparation and use of monoclonal antibodies to detect Phytophthora nicotianae var. nicotianaeOriginal Paper

Blanka Pekárová-Kyněrová, Miroslava Kutíková

A monoclonal antibody (MAb 18) was prepared against purified mycelial proteins from Phytophthora nicotianae var. nicotianae. The specificity of MAb 18 (lgG class) was tested using indirect ELISA (PTA-ELISA).It cross-reacted with Phytophthora cacto­ rum, P. cinrzamomi, P. cryptogea, P. fragariae) but not with other fungi (Fusarium oxysporum, Pythium ultimwn and P. oligan­ drwn) and bacteria (Clavibacter michiganensis subsp. michiganensis) isolated from tomato. Phytophthora nicotianae var. nicotianae was detected in roots and basal stems of artificially infected young tomato plants using indirect ELISA and immunoprinting.

Keywords: Phytophthora nicotianae var. nicotianae; monoclonal antibodies; indirect ELISA; immunoprinting; tomato

Published: June 30, 1999  Show citation

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Pekárová-Kyněrová B, Kutíková M. Preparation and use of monoclonal antibodies to detect Phytophthora nicotianae var. nicotianae. Plant Protect. Sci. 1999;35(2):41-46. doi: 10.17221/9673-PPS.
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