Plant Protection Science, 1999 (vol. 35), issue 2
Preparation and use of monoclonal antibodies to detect Phytophthora nicotianae var. nicotianaeOriginal Paper
Blanka Pekárová-Kyněrová, Miroslava Kutíková
Plant Protect. Sci., 1999, 35(2):41-46 | DOI: 10.17221/9673-PPS 
A monoclonal antibody (MAb 18) was prepared against purified mycelial proteins from Phytophthora nicotianae var. nicotianae. The specificity of MAb 18 (lgG class) was tested using indirect ELISA (PTA-ELISA).It cross-reacted with Phytophthora cacto rum, P. cinrzamomi, P. cryptogea, P. fragariae) but not with other fungi (Fusarium oxysporum, Pythium ultimwn and P. oligan drwn) and bacteria (Clavibacter michiganensis subsp. michiganensis)...
Silky bent grass (Apera spica-venti [L.] Beauv.) - a new host and reservoir of wheat dwarf virusOriginal Paper
Josef Vacke, Radim Cibulka
Plant Protect. Sci., 1999, 35(2):47-50 | DOI: 10.17221/9674-PPS
Wheat dwart virus (WDV) was detected by serological assays and transmission tests in samples of silky bent grass (Apera spica-venti [L.] Beauv.) that were taken in the field and showed signs of dwarfing, leaf yellowing and inflorescence reduction. This grass species is a new WDV host, not described until now. Transmission tests indicated that this grass is an efficeint source of infection for the vector of the virus, the leafhopper Psammotettix alienus Dahlb. Surveys at nine localities revealed a medium to high incidence of WDV in silky bent grass, which is a weed species frequently infesting cereal stands in the Czech Republic. As...
Wheat leaf rust races/pathotypes in the Czech Republic in 1997-1998Original Paper
Pavel Bartoš, Eva Stuchlíková
Plant Protect. Sci., 1999, 35(2):51-56 | DOI: 10.17221/9675-PPS
In 1997-1998 virulence of the wheat leaf rust population was studied on Thatcher near isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3. Lr9, Lrll, Lrl5, Lrl7, Lrl9, Lr21, Lr23, Lr24, Lr26 and Lr28. On these NILs 11 pathotypes were determined in 1997, and 8 inl998. All rust samples were avirulent onLr9, Lr19, Lr24 and Lr28.There was a relatively low frequency of virulence on Lrl, Lr2a and Lr2b. Most rust samples were virulent on Lr2c, Lr11,...
Virulence analyses of the powdery mildew population on wheat in the Czech Republic in 1995-1998Original Paper
Karel Klem
Plant Protect. Sci., 1999, 35(2):57-62 | DOI: 10.17221/9676-PPS
In 1995-1998, the frequency of selected virulences in the powdery mildew ( Blumeria graminis f. sp. tritici ) population on wheat in the Czech Republic was investigated. Samples of conidia were collected by a mobile version of a spore-trap. A total of 1739 one-colony isolates were analysed on a set of five varieties as differentials. High frequencies (> 50%) were found for virulences Vm2, Vm3a, Vm5 and Vml7, lower frequencies(< 50%) for combinations Vm9,2,1 and Vm2,6 and for Vm4b. There were no significant changes in virulence frequencies...
Effectiveness of fungicides against head blight of wheat caused by Fusarium culmorumOriginal Paper
Yahya Takish, Slavoj Vaverka
Plant Protect. Sci., 1999, 35(2):63-66 | DOI: 10.17221/9677-PPS
Fungicides were tested in 1996 and 1997 for their efficacy to control Fusarium culmorum after inoculation of winter wheat in the early flowering stage. The best results were achieved with metconazole and a mixture of tebuconazole with triadimefon or triadi menol. Good results were achieved with epoxiconazole, cyproconazole, flusilazol and bromuconazole.
Abundance of cereal aphids (Hemiptera: Aphididae) within wild Triticum and Aegilops spp., and cultivated wheatOriginal Paper
Helena Havlíčková, Vojtěch Holubec
Plant Protect. Sci., 1999, 35(2):67-70 | DOI: 10.17221/9678-PPS
Accessions of the wild Triticum species: T. boeticum, T. dicoccoides, T. urartu, and the Aegilops species: Ae. columnaris, Ae. geniculata, Ae. markgrafii, Ae. neglecta and Ae. triuncialis in the collection of the Gene Bank of RICP Prague-Ruzyně, with T. aestivum cv. Sandra as control, were evaluated for cereal aphid occurrence in the open during 1995-1998 period. The cereal aphid species Rhopalosiphum padi (RP), Metopolophium dirhodum (MD) and Sitobion avenae...
Early weed succession on an abandoned field: vegetation composition and production of biomassOriginal Paper
Jindra Štolcová, Alois Honěk
Plant Protect. Sci., 1999, 35(2):71-76 | DOI: 10.17221/9679-PPS
We investigated the early stage (second to fourth year) of weed succession on an experimental fallow field in central Bohemia (western Czech Republic). The experimental ground was ploughed in the autumn, harrowed and rolled in the spring, and divided into 5 x 5 m plots. In the control the weed stands were left intact, the other variants were mowed with or without cultivation of the superficial soil layer in June and July. The position of the plots and the treatments were the same each year. The production of aboveground biomass and proportion of monocotyledonous and dicotyledonous annuals and perennials in different years (1993 to 1995) were...
The sixty-fifth birthday of Prof. Dr. Anton J. Novacky, CSc.Ing. Josef Vacke, CSc., sedmdesátníkemBiographical Notice
Václav Kůdela, Zdenko Polák
Plant Protect. Sci., 1999, 35(2):77-78 | DOI: 10.17221/9680-PPS