Plant Protect. Sci., 2002, 38(1):1-12 | DOI: 10.17221/4813-PPS

Characterization and identification of entomopathogenic and mycoparasitic fungi using RAPD-PCR technique

Lenka Bieliková, Zdeněk Landa, Lance S. Osborne, Vladislav Čurn
Faculty of Agriculture - Department of Genetics and 2Faculty of Biology - Department of Plant Production, České Budějovice, Czech Republic; 3Mid-Florida Research & Educational Center, IFAS, University of Florida, Apopka, Florida, USA; 4University of South Bohemia, Faculty of Agriculture - Department of Fodder Plants, České Budějovice, Czech Republic

Entomopathogenic and mycoparasitic fungi were characterised by RAPD technique, with special attention to evaluate the genetic stability of strains that are used as active ingredients in commercial biopesticides. Strain-specific fingerprints were constructed for Paecilomyces fumosoroseus - strain PFR 97 Apopka, Gliocladium virens - strain GL 21 and Verticillium lecanii - strain MYCOTAL. Genetic stability and homogeneity was confirmed among re-isolates that were obtained from commercial batches of bio-insecticide PFR 97TM 20%WDG and bio-fungicide SoilGardTM12G that had been produced in 1995-1999. RAPD analysis indicated the genetic identity of V. lecanii strains re-isolated from the two different bio-insecticides MYCOTAL® and VERTALEC®. The usefulness of RAPD technique was demonstrated when P. fumosoroseus strain PFR 97 Apopka was reliably identified after having passed through adults of the spruce bark beetle Ips typographus, and by analysis of the relationship between fungi of the genus Gliocladium.

Keywords: Gliocladium spp.; Gliocladium virens; Paecilomyces fumosoroseus; Verticillium lecanii; entomopathogenic fungi; mycoparasitic fungi; identification using RAPD-PCR

Published: February 28, 2002  Show citation

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Bieliková L, Landa Z, Osborne LS, Čurn V. Characterization and identification of entomopathogenic and mycoparasitic fungi using RAPD-PCR technique. Plant Protect. Sci. 2002;38(1):1-12. doi: 10.17221/4813-PPS.
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