Plant Protect. Sci., 2002, 38(1):13-17 | DOI: 10.17221/4816-PPS

The application of RT-PCR assay for the detection of Apple stem pitting virus and Apple stem grooving virus in four apple cultivars

Jiban Kumar Kundu
Research Institute of Crop Production – Division of Plant Medicine, Prague-Ruzynì, Czech Republic

The reverse transcription polymerace chain reaction (RT-PCR) assay was successfully used for the detection of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in four apple cultivars of a 25 years old orchard. These two main pome fruit viruses were detected frequently in all tested apple cultivars. ASGV and ASPV occurred in as many as 16 trees (in the cultivar Spartan) and 13 trees (in the cultivar Idared) out of 20 tested trees, respectively. Mixed infection by ASGV and ASPV was found in all tested cultivars (as many as 9 out of 20 tested trees of the cultivar Spartan).

Keywords: RT-PCR; ASPV; ASGV; Apple stem pitting virus; Apple stem grooving virus

Published: February 28, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Kundu JK. The application of RT-PCR assay for the detection of Apple stem pitting virus and Apple stem grooving virus in four apple cultivars. Plant Protect. Sci. 2002;38(1):13-17. doi: 10.17221/4816-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. DESVIGNES C.J., BOYÉ R., CORNAGGIA D., GRASSEAU N. (1990): Maladies a virus des arbres fruitiers. Ctifl, Paris.
    2. FUCHS E., MERKER D., KEGLER G. (1979): Der Nachweis des chlorotischen Blattfleckungs-virus des Apfels (apple chlorotic leafspot virus), des Stammfurchungs-virus des Apfels (apple stem grooving virus) und des Tomatenzwergbuschvirus (tomato bushy stunt virus) mit dem ELISA. Arch. Phytopathol. Pflanzens., 15: 421–424. Go to original source...
    3. HOWELL W.E., MINK G.I., HURTT S.S., FORSTER J.A., POSTMEN J.D. (1996): Select Malus clones for rapid detection of apple stem grooving virus. Plant Disease, 80: 1200–1202. Go to original source...
    4. JAMES D. (1999): A simple and reliable protocol for the detection of apple stem grooving virus by RT-PCR and in a multiplex PCR assay. J. Virol. Methods, 83: 1–9. Go to original source... Go to PubMed...
    5. JANEÈKOVÁ M., PLUHAØ Z. (1987): Results of the ELISA procedure used for the detection of apple stem grooving virus (ASGV) in 24 isolates of ASGV, separated by heat therapy. Hort. Sci. (Prague), 14: 86–91.
    6. JELKMANN W. (1994): Nucleotide sequences of apple stem pitting virus and of the coat protein gene of a similar virus from pear associated with vein yellows disease and their relationship with potex- and carlaviruses. J. Gen. Virol., 75: 1535–1542. Go to original source... Go to PubMed...
    7. JELKMANN W., KEIM-KONRAD R. (1997): Immuno-captive polymerase chain reaction and plate-trapped ELISA for the detection of apple stem pitting virus. J. Phytopathol., 145: 499–503. Go to original source...
    8. KOGANEZAWA H., YANASE H. (1990): A new type of elongated virus isolated from apple trees containing the stem pitting agent. Plant Disease, 74: 610–614. Go to original source...
    9. KINARD G.R., SCOTT S.W., BARNETT O.W. (1996): Detection of apple chlorotic leaf spot and apple stem grooving viruses using RT-PCR. Plant Disease, 80: 616–621. Go to original source...
    10. KUMMERT J., MARINHO V. L.A., RUFFLARD G., COLINET D., LEPOIVRE P. (1998): Sensitive detection of apple stem grooving and apple stem pitting viruses from infected apple trees by RT-PCR. Acta Horticult., 472(1): 97–104. Go to original source...
    11. KUNDU J.K. (2001): To the diagnosis and distribution of the apple stem pitting virus in the Czech Republic. Acta Horticult., 550 (1): 275–280. Go to original source...
    12. LEONE G., LINDNER J.L., VAN DER MEER F.A., SCHOEN C.D. (1998): Symptoms on apple and pear indicators after backtransmission from Nicotiana occidentalis confirm the identity of apple stem pitting virus with pear vein yellows virus. Acta Hortic., 472: 61–65. Go to original source...
    13. MACKENZIE D.J., MCLEAN M.A., MUKERJI S., GREEN M. (1997): Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction. Plant Disease, 81: 222–226. Go to original source... Go to PubMed...
    14. MARINHO V.L.A., KUMMERT G., RUFFLARD G., COLINET D., LEPOIVRE P. (1998): Detection of apple stem grooving virus in dormant apple trees with crude extracts as templates for one-step RT-PCR. Plant Disease, 82: 785–790. Go to original source... Go to PubMed...
    15. MARTELLI G.P., JELKMANN W. (1998): Foveavirus, a new plant virus genus. Arch. Virology, 143: 1245–1249. Go to original source... Go to PubMed...
    16. MURPHY F.A., FAUQUET C.M., BISHOP D. H.L., GHABRIAL S.A., JARVIS A. W., MARTELLI G.P., MAYO M.A., SUMMERS M.D. (1995): Virus Taxonomy: The Classification and Nomenclature of Viruses. The Sixth Report of the International Committee on Taxonomy of Viruses. Springer, Vienna. Go to original source...
    17. NÉMETH M. (1986): Virus, Mycoplasma and Rickettsia Diseases of Fruit trees. Akademiai Kiadó, Budapest.
    18. POLÁK J., ZIEGLEROVÁ J. (2001): Distribution of apple stem grooving virus in apple trees in the Czech Republic. Plant Protect. Sci., 37:1–4. Go to original source...
    19. SCHWARZ K., JELKMANN W. (1998): Detection and characterization of European apple stem pitting virus sources from apple and pear by PCR and partial sequence analysis. Acta Hortic., 472: 75–85. Go to original source...
    20. SEIDL V., DROBKOVÁ R., KUÈEROVÁ V. (1979): Možnost využití nesystémového rozmístìní ASP-viru v korunì jabloní v ozdravovacím programu. Ochr. Rostl., 15: 232–234. Go to original source...
    21. STOUFFER R.F., FRIDLUND P.R. (1989): Indexing using woody indicators. In: Virus and Viruslike Diseases of Pome Fruits and Simulation Noninfectious Disorders. Washington State Univ. Cooper. Extension Serv., Pullman: 225–265.
    22. WELSH M.F., UYEMOTO J.K. (1980): Differentiation of syndromes caused in apple by graft-transmissible, xylem-affecting agents. Phytopathology, 70: 349–352. Go to original source...
    23. YANASE H., YAMAGUCHI A. (1982): Malus sieboldii MO-65 as a new indicator plant for apple stem pitting virus. Bull. Fruit Tree Res. Stn. Jpn., C9: 69–77.
    24. YOSHIKAWA N., SASAKI E., KATO M., TAKAHASHI T. (1992): The nucleotide sequence of apple stem grooving capillovirus genome. Virology, 191: 98–105. Recevied for publication September 27, 2001 Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content