Plant Protect. Sci., 2002, 38(10):S102-S103 | DOI: 10.17221/10329-PPS

Serine proteases in the extracellular preparations of Phytophthora infestans: does their presence relate to the aggressiveness of the pathogen?Original Paper

J. Hamill, C. Selby, L.R. Cooke
Department of Applied Plant Science, Queen's University, Belfast, BT9 5PX Northern Ireland, United Kingdom * Tel.: +44 28 9025 5254, Fax: +44 28 9066 8372, E-mail: j.a.hamill@qub.ac.uk

In this study the aggressiveness of nine isolates of P. infestans was determined using detached leaflets from cultivars Bintje and Stirling. The growth of the isolates on the leaflets was recorded on a daily basis, for seven days, and an assessment of their aggressiveness could then be made. Extracellular preparations (ECPs) from the zoospore suspension of each isolate were used as a source of proteolytic activity. The ECPs were found to contain a level of serine protease activity using BTEE (N-Benzoyl-L-Tyrosine Ethyl Ester) as a substrate and recording the absorbance at 256 nm. The possible relationship between the serine protease activity and the aggressiveness of the isolate is discussed.

Keywords: Phytophthora infestans; aggressiveness; serine protease

Published: June 30, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Hamill J, Selby C, Cooke LR. Serine proteases in the extracellular preparations of Phytophthora infestans: does their presence relate to the aggressiveness of the pathogen? Plant Protect. Sci. 2002;38(SI 1 - 6th Conf EFPP):S102-103. doi: 10.17221/10329-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. CARLISLE D.J., COOKE L.R., WATSON S. (2002): Foliar aggressiveness of Northern Ireland isolates of Phytophthora infestans population on detached leaflets of three potato cultivars.in Northern Ireland. Plant Pathol., 51: 424-435. Go to original source...
    2. CATEN C.E., JINKS J.L. (1968): Spontaneous variability of single spore isolates of Phytophthora infestans. I: Cultural variation. Can. J. Bot., 49: 329-348. Go to original source...
    3. HOLLOMAN D.W. (1965): A medium for the direct isolation of Phytophthora infestans. Plant Pathol., 14: 34-35. Go to original source...
    4. HUMMEL B.C.W. (1959): A modified spectrophotometric determination of chymotrypsin, trypsin and thrombin. Canad. J. Biochem. Physiol., 37: 1393-1399. Go to original source...
    5. KREGAR I., STRUKELJ B. (1999): Proteinase inhibitors offer the possibility of producing disease resistant transgenic potatoes. Food Technol. Biotechnol., 37: 39-44.
    6. LASKAWSKI M. JR., KATO I. (1980): Protein inhibitors of proteinases. Annu. Rev. Biochem., 49: 493-626. Go to original source... Go to PubMed...
    7. LORITO M., BROADWAY R.M., HAYES C.K., WOO S.L., NOVIELLO C., WILLIAMS D.L., HARMAN G.E. (1994): Proteinase inhibitors from plants as a novel class of fungicides. Mol. Plant Microbe In., 7: 525-527. Go to original source...
    8. PARIS R., LAMATTINA L. (1999): Phytophthora infestans secretes extracellular proteases with necrosis inducing activity on potato. Eur. J. Plant Pathol., 105: 753-760. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content