Plant Protect. Sci., 2002, 38(10):S155-S157 | DOI: 10.17221/10343-PPS

Turnip mosaic virus determinants of virulence for Brassica napus resistance genesOriginal Paper

C.E. Jenner, F. Sánchez, K. Tomimura, K. Ohshima, F. Ponz, J.A. Walsh
1 Horticulture Research International, Wellesbourne, Warwick, CV35 9EF, United Kingdom
2 INIA,
8 040, Madrid, Spain
3 Laboratory of Plant Virology, Saga University, Saga 840-8502, Japan Tel.: +44 1789 470 382, Fax: +44 1789 470 552, E-mail: carol.jenner@hri.ac.uk$2

Dominant resistance genes identified in Brassica napus lines are effective against some, but not all, Turnip mosaic virus(TuMV) isolates. An infectious clone of an isolate (UK 1) was used as the basis of chimeric virus constructions usingresistance-breaking mutants and other isolates to identify the virulence determinants for three dominant resistance genes.For the resistance gene TuRB01, the presence of either of two mutations affecting the cylindrical inclusion (CI) proteinconverted the avirulent UK 1 to a virulent isolate. Acquisition of such mutations had a slight cost to viral fitness inplants lacking the resistance gene. A similar strategy is being used to identify the virulence determinants for two moreresistance genes present in another B. napus line.

Keywords: Turnip mosaic virus; Brassica napus; resistance genes; pathogenicity determinants

Published: June 30, 2002  Show citation

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Jenner CE, Sánchez F, Tomimura K, Ohshima K, Ponz F, Walsh JA. Turnip mosaic virus determinants of virulence for Brassica napus resistance genes. Plant Protect. Sci. 2002;38(SI 1 - 6th Conf EFPP):S155-157. doi: 10.17221/10343-PPS.
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