Plant Protect. Sci., 2003, 39(3):93-96 | DOI: 10.17221/3863-PPS

Detection of Apple stem grooving virus in different tissues of apple trees throughout the year

Jiban Kumar. Kundu, Jiří Svoboda, Jaroslav Polák
Department of Virology, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic

The suitability of different apple tissues for Apple stem grooving virus (ASGV) detection throughout the year was checked by RT-PCR and ELISA. Detectable amounts of ASGV were generally found in all tissues (bark, dormant buds, petals and leaves) tested by RT-PCR from January to mid-June. Leaves during flowering (in May) were the most suitable tissues for the virus detection by both methods (RT-PCR and ELISA). The leaves collected in summer (June, July and August) or other tissues such as bark, dormant buds and petals were not reliable for ASGV detection by ELISA.

Keywords: Apple stem grooving virus; tissue sampling; RT-PCR; ELISA; reliability of detection

Published: September 30, 2003  Show citation

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Kundu JK, Svoboda J, Polák J. Detection of Apple stem grooving virus in different tissues of apple trees throughout the year. Plant Protect. Sci. 2003;39(3):93-96. doi: 10.17221/3863-PPS.
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    References

    1. C A.I. (1963): The effect of some latent virus infections on the growth and cropping of apples. J. Hort. Sci., 38: 15-19. Go to original source...
    2. D C.J., B R., C D., G N. (1990): Maladies a virus des arbres fruitiers. Ctifl, Paris: 127.
    3. F C.L., C M.F. (1979): The detection of apple chlorotic leaf spot virus by a modified procedure enzyme-linked immunosorbent assay (ELISA). Ann. Appl. Biol., 91: 61-65. Go to original source...
    4. F E. (1981): Serological detection of Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in apple trees. Acta Hort., 94: 69-73. Go to original source...
    5. F E. (1982): Studies of the development of concentration of Apple chlorotic leaf spot virus (CLSV) and Apple stem grooving virus (SGV) in apple trees. Acta Phytopath. Acad. Sci. Hung., 17: 23-27.
    6. J D. (1999): A simple and reliable protocol for the detection of Apple stem grooving virus by RT-PCR and in a multiplex PCR assay. J. Virol. Methods, 83: 1-9. Go to original source... Go to PubMed...
    7. K J.K. (2002): The application of RT-PCR assay for the detection of Apple stem pitting virus and Apple stem grooving virus in four apple cultivars. Plant Protect. Sci., 38:13-17. Go to original source...
    8. K J.K. (2003): The occurrence of Apple stem pi ing virus and Apple stem grooving virus within field-grown apple cultivars evaluated by RT-PCR. Plant Protect. Sci., 39: 88-92. Go to original source...
    9. M K D.J., M L M.A., M S., G M. (1997): Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction. Plant Dis., 81: 222-226. Go to original source... Go to PubMed...
    10. M D., K A. (1993): Isolation of RNA from apple skin. Plant Mol. Biol. Reptr., 11: 326-332. Go to original source...
    11. N M. (1986): Virus, Mycoplasma and Ricke sia Diseases of Fruit Trees. Akademiai Kiado, Budapest.
    12. P J. (1995): Reliability of detection and relative concentration of plum pox virus determined by ELISA in an infected peach tree during the vegetation period. J Plant Dis. Protect., 102 :16-22.
    13. P J., Z J. (2001): Distribution of Apple stem grooving virus in apple trees in the Czech Republic. Plant Protect. Sci., 37: 1-4. Go to original source...
    14. S S., N A. (2001): RT-PCR based detection of Rupestris stem pi ing associated virus within field-grown grapevines throughout the year. Plant Dis., 85: 617-620. Go to original source... Go to PubMed...
    15. Y H., M G.I., S K., Y A. (1990): Apple topworking disease. In: J A.L., A H.S. (eds): Compendium of Apple and Pear Diseases. APS, St. Paul, MN: 74-75.
    16. Y N., S E., K M., T T. (1992): The nucleotide sequence of apple stem grooving capillovirus genome. Virology, 191: 98-105. Go to original source... Go to PubMed...

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