Plant Protection Science, 2003 (vol. 39), issue 3

Induction of post-infection ethylene and its role in resistance of bean (Phaseolus vulgaris) against Colletotrichum lindemuthianum

James Ombiri, Volker Zinkernagel, Eliud M. Gathuru, Oliver Achwanya, Aleš Lebeda

Plant Protect. Sci., 2003, 39(3):79-87 | DOI: 10.17221/3861-PPS  

The influence of inoculation techniques on induction of post-infection ethylene production in susceptible and resistant genotypes of bean (Phaseolus vulgaris) inoculated with the kappa race of Colletotrichum lindemuthianum was studied. Three inoculation methods (brushing, dipping and spraying) of excised leaves were compared. The brushing technique caused both the highest ethylene production at 120 h after inoculation, and better symptom development than dipping and spraying. It was, therefore, adopted to determine the post-infection ethylene production in four inoculated bean genotypes (GLP406 and Kaboon - resistant, GLP636 - moderately...

The occurrence of Apple stem pitting virus and Apple stem grooving virus within field-grown apple cultivars evaluated by RT-PCR

Jiban Kumar Kundu

Plant Protect. Sci., 2003, 39(3):88-92 | DOI: 10.17221/3862-PPS  

The reverse transcription polymerase chain reaction (RT-PCR) was successfully used to determine the occurrence of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in field-grown apple cultivars. Both viruses were detected frequently in all 16 tested apple cultivars. As many as 27.86% ASPV-infected and 44% ASGV-infected trees were recorded among a total of 420 tested trees from 15 different orchards. Mixed infection with ASGV and ASPV was recorded in 16.7% of the trees.

Detection of Apple stem grooving virus in different tissues of apple trees throughout the year

Jiban Kumar. Kundu, Jiří Svoboda, Jaroslav Polák

Plant Protect. Sci., 2003, 39(3):93-96 | DOI: 10.17221/3863-PPS  

The suitability of different apple tissues for Apple stem grooving virus (ASGV) detection throughout the year was checked by RT-PCR and ELISA. Detectable amounts of ASGV were generally found in all tissues (bark, dormant buds, petals and leaves) tested by RT-PCR from January to mid-June. Leaves during flowering (in May) were the most suitable tissues for the virus detection by both methods (RT-PCR and ELISA). The leaves collected in summer (June, July and August) or other tissues such as bark, dormant buds and petals were not reliable for ASGV detection by ELISA.

Hymenopteran parasitoids (Hymenoptera: Aphidiidae) of cereal aphids (Sternorrhyncha: Aphidoidea) in winter wheat crops in Slovakia

Ján Praslička, Shoki Al Dobai, Jozef Huszár

Plant Protect. Sci., 2003, 39(3):97-102 | DOI: 10.17221/3864-PPS  

During 1997-1999, occurrence of hymenopteran parasitoids of cereal aphids was observed in different localities in Slovakia. Altogether, seven species of aphid parasitoids were recorded. The total numbers of particular species over the last three years were as follows: Aphidius uzbekistanicus (160 individuals - 42.4%), Aphidius ervi (83 individuals - 22.0%), Aphidius rhopalosiphi (66 individuals - 17.5%), Ephedrus plagiator (40 individuals - 10.6%), Praon volucre (26 individuals - 6.9%), P. gallicum (1 individual - 0.3%), and Aphidius picipes (1 individual - 0.2%). The abundance...

Acute toxicity of carbofuran to selected species of aquatic and terrestrial organisms

Radka Dobšíková

Plant Protect. Sci., 2003, 39(3):103-108 | DOI: 10.17221/3865-PPS  

Carbofuran is an anticholinesterase carbamate commonly used as an insecticide, nematicide and acaricide in agricultural practice throughout the world. The aim of the study was to investigate the acute toxicity of carbofuran to selected species of aquatic organisms (the guppy Poecilia reticulata Peters, the water flea Daphnia magna Straus and the green alga Raphidocelis subcapitata Korsikov), and to a terrestrial organism (a white mustard Sinapis alba Linné). Daphnia magna Straus was found to be the most sensitive organism.

Scientometrical analysis of journal Plant Protection Science in 1950-2002

Václav Stejskal, Radek Aulický

Plant Protect. Sci., 2003, 39(3):109-115 | DOI: 10.17221/3866-PPS  

We analysed scientific papers published in the "Plant Protection Science" (PPS), former "Ochrana Rostlin" which is the only international scientific journal covering the problematic of the plant protection in the Czech Republic (CZ). The aim of this study was to explore general trends in the plant protection research in CZ during the five past decades (i.e. 1950-2002). During the period studied, 1633 articles and 2425 authors appeared in PPS. The peak of the annual publishing quantity was in 1970s and 1980s. The number of papers per year declined in 1990s reflecting (i) a decrease of scientific institutes and restriction of agricultural research in...