Plant Protect. Sci., 2004, 40(1):1-4 | DOI: 10.17221/3120-PPS

Identification of Xiphinema vuittenezi by polymerase chain reaction

Shesh Kumari, Jiban K. Kundu, Jaroslav Polák
Department of Virology, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic

So far, the identification of the nematode species Xiphinema vuittenezi relied mainly on time-consuming morphological and morphometrical studies. Therefore, a polymerase chain reaction (PCR) protocol was optimised that both reliably and rapidly identifies X. vuittenezi. The internal transcribed spacer (ITS) species-specific primer of ribosomal DNA gene of X. vuittenezi was used. Nine populations of this species from Central Bohemia were investigated by means of PCR.

Keywords: nematodes; Xiphinema vuittenezi; PCR; ITS primer

Published: March 31, 2004  Show citation

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Kumari S, Kundu JK, Polák J. Identification of Xiphinema vuittenezi by polymerase chain reaction. Plant Protect. Sci. 2004;40(1):1-4. doi: 10.17221/3120-PPS.
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