Plant Protect. Sci., 2004, 40(4):128-134 | DOI: 10.17221/467-PPS

Inhibitory effect of Pseudomonas spp. on the development of Botrytis cinerea and Penicillium expansum

Hanna Bryk, Barbara Dyki, Piotr Sobiczewski
1 Research Institute of Pomology and Floriculture, Skierniewice, Poland
2 Research Institute of Vegetable Crops, Skierniewice, Poland

The influence of antagonistic Pseudomonas spp. on the development of Botrytis cinerea and Penicillium expansum was studied in liquid cultures. Two strains of Pseudomonas spp. (B194 and B224), originally isolated from apple leaves and fruits, respectively, inhibited spore germination and germ tube elongation of B. cinerea or P. expansum. The inhibitory effect depended on the concentration of bacteria in the cultures. After a prolonged time of incubation (48-76 h) lysis and fragmentation of hyphae of both fungi was observed. In some cases the hyphae of B. cinerea developed abnormally if the bacterial strains were present - the hyphal tips were swollen and ball-shaped spore-like structures aggregated in chains were formed.

Keywords: biocontrol; blue mold; gray mold; microbial interaction; mechanism of biocontrol

Published: December 31, 2004  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Bryk H, Dyki B, Sobiczewski P. Inhibitory effect of Pseudomonas spp. on the development of Botrytis cinerea and Penicillium expansum. Plant Protect. Sci. 2004;40(4):128-134. doi: 10.17221/467-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. A G. (1996): Mode of action of an isolate of Candida famata in biological control of Penicillium digitatum in orange fruits. Posth. Biol. and Tech., 8: 191-198. Go to original source...
    2. B R.R., D J.W. (1996): Interaction specificity of the biocontrol agent Sporothrix flocculosa: A video microscopy study. Phytopathology, 86: 1317-1323. Go to original source...
    3. B J.F. (1988): Identification of cultivable bacteria from plants and plant tissue cultures by use of simple classical methods. Acta Horticult., 225: 27 -37. Go to original source...
    4. B H., D B., S P. (1998): Antagonistic effect of Erwinia herbicola on in vitro spore germination and germ tube elongation of Botrytis cinerea and Penicillium expansum. BioControl, 43: 97-106. Go to original source...
    5. B H., S P., B S. (1999): Evaluation of protective activity of epiphytic bacteria against gray mold (Botrytis cinerea) and blue mold (Penicillium expansum) on apples. Phytopathol. Pol., 18: 69-79.
    6. E G A., W C., W M. (1998): Ultrastructural and cytochemical aspect of the biocontrol activity of Candida saitoana in apple fruit. Phytopathology, 88: 282-291. Go to original source... Go to PubMed...
    7. J W.J. (1987). Postharvest biological control of blue mold on apples. Phytopathology, 77: 481-485. Go to original source...
    8. J W.J., M A. (1992): Control of storage rots on various pear cultivars with a saprophytic strain of Pseudomonas syringae. Plant Dis., 76: 555-560. Go to original source...
    9. J W.J., R J. (1988): Biological control of blue mold and grey mold on apple and pear with Pseudomonas cepacia. Phytopathology, 78: 1697-1700. Go to original source...
    10. J W.J., T T.J., S C. (2000): Characterizing the mechanism of biological control of postharvest diseases on fruits with a simple method to study competition for nutrients. Phytopathology, 90: 1196-1200. Go to original source... Go to PubMed...
    11. J W.J., K L. (2002): Biological control of postharvest diseases of fruits. Annu. Rev. Phytopathol. 40: 411-441. Go to original source... Go to PubMed...
    12. M G.H., C R.A. (1989): Postharvest biocontrol of grey mould of pear by Pseudomonas gladioli. Phytopathology, 79: 1153.
    13. N E.B., C W.L., N J.M., N G.T., H G.E. (1986): A achment of Enterobacter cloacae to hyphae of Pythium ultimum: possible role in the biological control of Pythium pre-emergence damping-off. Phytopathology, 76: 327-335. Go to original source...
    14. P P.L., W C.L. (1984): Postharvest biological control of stone fruit brown rot by Bacillus subtilis. Plant Dis., 68: 753-756. Go to original source...
    15. R E., L S., D S., I Z., C I., C L. (2002): Biocontrol of postharvest fungal pathogens of peaches and apples by Pantoae agglomerans strain ICI1270. IOBC WPRS Bull., 25 (10): 199-202.
    16. S J.L., D -A R., B J.R., G A.R., H D., J W.J. (1993): Biocontrol of postharvest brown rot of nectarines and peaches by Pseudomonas species. Crop Protect., 12: 513-520. Go to original source...
    17. S P., B H., B S. (1996): Evaluation of epiphytic bacteria isolated from apple leaves in the control of postharvest apple diseases. J. Fruit Ornam. Plant Res., IV: 35-45.
    18. U R.S., S P.L. (1986): In vitro inhibition of plant pathogens: Bacillus subtilis and Enterobacter aerogenes in vitro control of two postharvest cherry diseases. Can. J. Microb., 32: 963-967. Go to original source...
    19. W C.L., C E. (1989): Postharvest biological control of Penicillium rots of citrus with antagonistic yeasts and bacteria. Sci. Horticult., 40: 105-112. Go to original source...
    20. W C.L., F J.D., P P.L. (1987): Biological control of Rhizopus rot of peach with Enterobacter cloacae. Phytopathology, 77: 303-305. Go to original source...
    21. W M., W C.L., H W. (1989): Characterization of inhibition of Rhizopus stolonifer germination and growth by Enterobacter cloacae. Can. J. Bot., 67: 2317-2323. Go to original source...
    22. W M., B C.L., D S., M L R., W C.L., C E. (1991): Mode of action of postharvest biocontrol yeast, Pichia guilliermondii. I: Characterization of a achment to Botrytis cinerea. Physiol. Mol. Plant Pathol., 39: 245-258. Go to original source...
    23. W J.M. (1992). Status of biological disease control in horticulture. Biocontrol Sci. Technol., 2: 3-24. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content