Plant Protect. Sci., 2005, 41(2):52-57 | DOI: 10.17221/2747-PPS
Isolation of pathogenesis-related proteins from TMV-infected tobacco and their influence on infectivity of TMV
- Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic
The composition of pathogenesis-related proteins (PR-proteins) in the intercellular fluid (ICF) and leaf tissue of the hypersensitive tobacco cultivar Xanthi-nc inoculated with Tobacco mosaic virus (TMV), and their inhibitory influence on TMV multiplication were studied. The ICF PR-proteins of infected plants were separated after solubilisation by decreasing gradient of ammonium sulphate, the cell PR-proteins were separated after acidic homogenisation of leaf tissues. The ICF and cell PR-proteins were further purified by ion exchange chromatography on DEAE cellulose. Using discontinuous non-denaturating polyacrylamide gel electrophoresis of DEAE cellulose fractions the PR-proteins were detected. Their molecular weights were estimated by SDS-PAGE. The ICF and cell proteins of infected leaves included PR-proteins of the molecular weights 15-16 kDa (Group 1), 27-28 kDa (Group 3: chitinases) and 36-40 kDa (Group 2a: ڨ-1,3-glucanases). Fractions with different PR-proteins were tested for their effect on infectivity of TMV. Particularly the PR3 and PR2a proteins seem to decrease the infectivity of TMV.
Keywords: PR-proteins; PAGE; Tobacco mosaic viru
Published: June 30, 2005 Show citation
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