Plant Protection Science, 2005 (vol. 41), issue 2
Prune cv. Jojo resistance to different strains of Plum pox virus
Jaroslav Polák, Jitka Pívalová, Jiří Svoboda
Plant Protect. Sci., 2005, 41(2):47-51 | DOI: 10.17221/2742-PPS 
Trees of prune (Prunus domestica L.), cv. Jojo, were inoculated by chip budding with three different strains of PPV isolated from European plum in the Czech Republic. These isolates included Plum pox virus M strain (PPV-M), Plum pox virus D strain (PPV-D) and a PPV-recombinant both strains (PPV-Rec). The results of the evaluation of the inoculated trees over 2 years are presented. Trees of plum cv. Jojo behaven differently to infection with the three PPV strains. A strong hypersensitive reaction appeared a year after inoculation with PPV-M and PPV-Rec strains, although not all inoculated tree died. PPV must have been present in the...
Isolation of pathogenesis-related proteins from TMV-infected tobacco and their influence on infectivity of TMV
Milada Šindelářová, Luděk Šindelář
Plant Protect. Sci., 2005, 41(2):52-57 | DOI: 10.17221/2747-PPS
The composition of pathogenesis-related proteins (PR-proteins) in the intercellular fluid (ICF) and leaf tissue of the hypersensitive tobacco cultivar Xanthi-nc inoculated with Tobacco mosaic virus (TMV), and their inhibitory influence on TMV multiplication were studied. The ICF PR-proteins of infected plants were separated after solubilisation by decreasing gradient of ammonium sulphate, the cell PR-proteins were separated after acidic homogenisation of leaf tissues. The ICF and cell PR-proteins were further purified by ion exchange chromatography on DEAE cellulose. Using discontinuous non-denaturating polyacrylamide gel electrophoresis of DEAE cellulose...
Variation in the production of trichothecene mycotoxin deoxynivalenol (DON) in spring barley varieties after treatment with the fungicides azoxystrobin and tebuconazole
Josef Hýsek, Marie Váňová, Jana Hajšlová, Jana Brožová, Eliška Sychrová, Zuzana Radová-Sypecká, Václav Šíp, Světlana Sýkorová, Jana Chrpová, Ludvík Tvarůžek
Plant Protect. Sci., 2005, 41(2):58-62 | DOI: 10.17221/2745-PPS
Eight varieties of spring barley (Hordeum vulgaris Lin.) were artificially inoculated with a Fusarium culmorum (W.G. Smith) Saccardo - isolate and naturally infected in the middle of the flowering period, and 2 d later treated with the fungicides azoxystrobin or tebuconazol at a dose of 1 l/ha in 250 l of water. In both control and treated samples of grain the content of deoxynivalenol (DON), the main trichothecene mycotoxin produced by F. culmorum, was determined by gas chromatography (GC-ECD). The treatment with either fungicide resulted in elevated levels of DON, an effect that was more pronounced with azoxystrobin.
Effect of two NeemAzalTM formulations on honeybees under semi-field conditions
Shawki Mahmoud Abd-Allah, Vladimír Táborský, František Kamler, Jan Kazda
Plant Protect. Sci., 2005, 41(2):63-72 | DOI: 10.17221/2744-PPS
The effects of NeemAzalTM formulations: NeemAzalTM T/S (1% azadirachtin) and NeemAzalTM granules (1% azadirachtin) on honeybees, Apis mellifera L., were studied under semi-field conditions. Three plots at 15 m2 each were sown with spring rape seeds Brassica napus cultivar Likolly (Brassicaceae/Cruciferae). In the first plot NeemAzal granules were added with the seeds during sowing. The second plot was sprayed with NeemAzal T/S during full flowering; GreemaxTM was used as a wetting agent. The third one was sprayed with water only during full flowering as a control. For each treatment one tunnel tent (3 × 5 × 2 m) was used during...
Infestation of poppy cultures with the poppy stem gall wasp (Timaspis papaveris) Cynipidae: Hymenoptera
Josef Šedivý, Pavel Cihlař
Plant Protect. Sci., 2005, 41(2):73-79 | DOI: 10.17221/2746-PPS
The mortality of pupae in stems lying on the ground during hibernation was 51.7% in 2003. The emergence of adults in 2003 and 2004 was monitored from late April to early July by photoeclectors placed on fields that grew poppy the previous year. Most adult gall wasps emerged in April to early May 2004. The occurrence of females ovipositing on poppy stems varied from April to July and the degree of damage to the plants increased. Most frequently the females oviposited in the first stem internodes. The infestation of poppy cultures with the gall wasps was almost uniform, the number of infested plants increasing still in June. In late June, only seven...
Current occurrence and distribution of heterodera avenae in the Slovak Republic
Marek Renčo
Plant Protect. Sci., 2005, 41(2):80-85 | DOI: 10.17221/2740-PPS
During 2003 and 2004 the occurrence and distribution of Heterodera avenae (Woll.) in the cereal growing areas of the Slovak Republic was studied. 188 soil samples from 27 districts throughout the country were analysed; Heterodera avenae was present in 56.4% of the samples, at an incidence of 2-81 cysts in 100 g soil. At 87 localities the incidence of the parasite was low, at 15 it was medium, and high at 4 localities.
Outdoor occurrence of stored-product pests (Coleoptera) in the vicinity of a grain store - Short communication
Zuzana Kučerová, Radek Aulický, Václav Stejskal
Plant Protect. Sci., 2005, 41(2):86-89 | DOI: 10.17221/2739-PPS
The species composition, frequency and abundance of insect pests (Coleoptera) occurring outside a grain store were explored. Wheat bait traps were used for pest monitoring; they were changed every month from April to September. For the first time the outdoor occurrence of primary and secondary stored-product pests was documented in the Czech Republic. Seven species of Coleoptera were found outside the grain store. Pest abundance and frequency were in high correlation. The primary pest Sitophilus granarius was dominant.
Powdery mildew Phyllactinia corni causing disease on Cornus mas (Cornaceae) - a new record for Slovakia
Kamila Bacigálová, Dezider Tóth, Ján Brindza
Plant Protect. Sci., 2005, 41(2):90-93 | DOI: 10.17221/2741-PPS
Phyllactinia corni, a powdery mildew on Cornus mas, is reported for the first time from Slovakia. The conidial state and mature cleistothecia found at two locations in Slovakia are described and illustrated.
Prof. Ing. ZDENĚK ČAČA, DrSc. (1927-2005) - Obituary notice
Karel Veverka
Plant Protect. Sci., 2005, 41(2):94 | DOI: 10.17221/2743-PPS