Plant Protect. Sci., 2014, 50(4):174-178 | DOI: 10.17221/84/2013-PPS

Blueberry red ringspot virus eliminated from highbush blueberry by shoot tip cultureOriginal Paper

Josef ©PAK1, Daniela PAVINGEROVÁ1, Jaroslava PŘIBYLOVÁ1, Vlastimila ©PAKOVÁ1, Frantiąek PAPR©TEIN2, Jiří SEDLÁK2
1 Institute of Plant Molecular Biology, Biology Centre, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
2 Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech Republic

In order to find an effective elimination method of Blueberry red ringspot virus (BRRV), shoot tips of 2-3 mm size originating from buds of highbush blueberry cv. Darrow naturally infected by BRRV and collected in winter months were regenerated and multiplied on woody-plant medium (WPM) with 1.5 mg/l zeatin and 20 g/l sucrose. Developed shoots tested negative by polymerase chain reaction (PCR) for BRRV were transferred onto a rooting medium consisting of WPM supplemented with 1 mg/l indole-3-butyric acid. Rooting plants were transferred into soil and tested twice by PCR 6 months apart. Ninety per cent eradication of BRRV was achieved while isolating shoot tips, multiplying them in one vegetative generation, and regenerating whole 50-60 cm high plants within 30 months. All plants developed rich fruits after their overwintering in a cold greenhouse and were tested BRRV negative in summer 2013.

Keywords: BRRV; in vitro; PCR; Vaccinium corymbosum L.; virus-free; virus elimination

Published: December 31, 2014  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
©PAK J, PAVINGEROVÁ D, PŘIBYLOVÁ J, ©PAKOVÁ V, PAPR©TEIN F, SEDLÁK J. Blueberry red ringspot virus eliminated from highbush blueberry by shoot tip culture. Plant Protect. Sci. 2014;50(4):174-178. doi: 10.17221/84/2013-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Cheong E.J., Mock R., Li R. (2012): Elimination of five viruses from sugarcane using in vitro culture of axillary buds and apical meristems. Plant Cell Tissue and Organ Culture, 109: 439-445. Go to original source...
    2. Cline W.O., Ballington J.R., Polashock J.J. (2008): Blueberry red ringspot observations and findings in North Carolina. Acta Horticulturae (ISHS), 810: 305-312. Go to original source...
    3. Glasheen B.M., Polashock J.J., Lawrence D.M., Gillett J.M., Ramsdell D.C., Vorsa N., Hillman B.I. (2002): Cloning, sequencing, and promoter identification of Blueberry red ringspot virus, a member of the family Caulimoviridae with similarities to the "Soybean chlorotic mottle-like" genus. Archives of Virology, 147: 2169-2186. Go to original source... Go to PubMed...
    4. Hutchinson M., Varney E.H. (1954): Ringspot-A virus disease of cultivated blueberry. Plant Disease Reporter, 38: 260-262.
    5. Isogai M., Ishii K., Umemoto S., Watanabe M., Yoshikawa N. (2009): First report of blueberry red ringspot disease caused by Blueberry red ringspot virus in Japan. Journal of General Plant Pathology, 75: 140-143. Go to original source...
    6. Kalinowska E., Paduch-Cichal E., Chodorska M. (2012): Molecular characterization of polish blueberry red ringspot virus isolate. Virus Genes, 44: 309-311. Go to original source... Go to PubMed...
    7. Kim K.S., Ramsdell D.C., Gillett J.M., Fulton J.P. (1981): Virions and ultrastructural changes associated with blueberry red ringspot diseases. Phytopathology, 71: 673-678. Go to original source...
    8. Lloyd G., McCown B. (1980): Commercially-feasible micropropagation of Mountain laurel, Kalmia latifolia, by use of shoot tip culture. Combined Proceedings, International Plant Propagators' Society, 30: 421-427.
    9. Mavrič Pleąko M., Virscek Marn M.V., Koron D. (2009): Detection of Blueberry red ringspot virus (BRRV) in highbush blueberry cv. 'Coville' in Slovenia. In: Book of Abstracts. 21 st International Conference on Virus and Other Graft Transmissible Diseases of Fruit Crops, Germany, Neustadt, July 5-10, 2009.
    10. Ostrolucká M.G., Gajdoąová A., Libiaková G., Hrubíková K., Beľo M. (2007): Protocol for micropropagation of selected Vaccinium spp. In: Jain S.M., Häggman H. (eds): Protocols for Micropropagation of Woody Trees and Fruits. Springer Science & Business Media, Netherlands: 445-455. Go to original source...
    11. Petrzik K., Přibylová J., Mavrič Pleąko I., ©pak J. (2011): Complete genome sequences of blueberry red ringspot virus (Caulimoviridae) isolates from the Czech Republic and Slovenia. Archives of Virology, 156: 1901-1903. Go to original source... Go to PubMed...
    12. Polashock J.J., Ehlenfeldt M.K. (2009): Molecular detection and discrimination of Blueberry red ringspot virus strains causing disease in cultivated blueberry and cranberry. Plant Disease, 93: 727-733. Go to original source... Go to PubMed...
    13. Přibylová J., ©pak J., Kubelková D., Petrzik K. (2010): First report of Blueberry red ringspot virus in highbush blueberry in the Czech Republic. Plant Disease, 94: 1071. Go to original source... Go to PubMed...
    14. Ramsdell D.C., Kim K.S., Fulton J.P. (1987): Red ringspot of blueberry. In: Converse R.H. (ed.): Virus Diseases of Small Fruits, Agriculture Handbook No 631. United States Department of Agriculture, Washington: 121-123.
    15. Stretch A.W., Scott D.H. (1977): Production of highbush blueberries free of red ringspot disease. Plant Disease Reporter, 61: 605-607.
    16. ©pak J., Votruba I., Pavingerová D., Holý A., ©paková V., Petrzik K. (2012): Antiviral activity of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP and ribavirin against Cauliflower mosaic virus in Brassica pekinensis. Plant Cell Tissue and Organ Culture, 110: 63-68. Go to original source...
    17. Tan R., Wang L., Hong N., Wang G. (2010): Enhanced efficiency of virus eradication following thermotherapy of shoot-tip cultures of pear. Plant Cell Tissue and Organ Culture, 101: 229-235. Go to original source...
    18. Wang Q.C., Valkonen J.P.T. (2008): Elimination of two viruses which interact synergistically from sweetpotato by shoot tip culture and cryotherapy. Journal of Virological Methods, 154: 135-145. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content