Plant Protect. Sci., 2014, 50(4):217-220 | DOI: 10.17221/17/2014-PPS

Occurrence and genetic similarity of Diplodia pinea on shoots and cones in seed orchards of Pinus spp. in north-western TurkeyOriginal Paper

Tuğba H. DOĞMUª-LEHTIJÄRVI1, Ayºe Gülden Aday KAYA2, Asko LEHTIJÄRVI3, Funda OSKAY4, Özgür Durmuº KAYA1
1 Faculty of Forestry, Süleyman Demirel University, Isparta, Turkey
2 Yeniºarbademli Vocational School, Süleyman Demirel University, Isparta, Turkey
3 Faculty of Forestry, Bursa Technical University, Bursa, Turkey
4 Faculty of Forestry, Çankiri Karatekin University, Çankiri, Turkey

Diplodia shoot blight disease can cause significant damage on coniferous trees and be particularly injurious to cones, which reduces the amount of seed production and germination. We investigated the disease severity and genetic variation of Diplodia pinea in one Pinus nigra and two P. sylvestris seed orchards. Disease surveys were carried out in İzmit (Marmara region, Turkey) in May 2012. Symptomatic shoots and cones were examined for the presence of pycnidia. Cultural and morphological characteristics of the isolates were studied using cultures grown on potato dextrose agar (PDA). Based on morphological characteristics and results using species specific primers, the pycnidia on shoots and cones were identified as D. pinea. In addition, Random Amplified Microsatellite Sequence (RAMS) analyses indicated that there was a single genet of D. pinea which caused the disease in the seed orchards. All of the 60 sampled trees were found to be infected by the fungus. There were differences in disease severity among the stands.

Keywords: diplodia shoot blight; pine; RAMS; disease severity

Published: December 31, 2014  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
DOĞMUª-LEHTIJÄRVI TH, KAYA AGA, LEHTIJÄRVI A, OSKAY F, KAYA ÖD. Occurrence and genetic similarity of Diplodia pinea on shoots and cones in seed orchards of Pinus spp. in north-western Turkey. Plant Protect. Sci. 2014;50(4):217-220. doi: 10.17221/17/2014-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Kaya A.G.A., Lehtijärvi A., Kaya Ö.D., DoğmuºLehtijärvi H.T. (2014): First report of Diplodia pinea on Pseudotsuga menziesii in Turkey. Plant Disease, 98: 689. Go to original source... Go to PubMed...
    2. Brookhouser L.W., Peterson G.W. (1971): Infection of Austrian, Scots and Ponderosa pines by Diplodia pinea. Phytopathology, 6: 439-444. Go to original source...
    3. Burgess T.I., Wingfield M.J., Wingfield B.D. (2004): Global distribution of Diplodia pinea genotypes revealed using simple sequence repeat (SSR) markers. Australasian Plant Pathology, 33: 513-519. Go to original source...
    4. Chou C.K.S. (1976): A shoot dieback in Pinus radiata caused by Diplodia pinea II. Inoculation studies. New Zealand Journal of Forestry Science, 6: 409-420.
    5. Chou C.K.S. (1987): Crown wilt of Pinus radiata associated with Diplodia pinea infection of woody stems. European Journal of Forest Pathology, 17: 398-411.
    6. Chou C.K.S., MacKenzie M. (1988): Effect of pruning intensity and season on Diplodia pinea infection of Pinus radiata stems through pruning wounds. European Journal of Forest Pathology, 18: 437-444. Go to original source...
    7. De Wet J., Wingfield M. J., Coutinho T.A., Wingfield B.D. (2000): Characterization of Sphaeropsis sapinea isolates from South Africa, Mexico, and Indonesia. Plant Disease, 84: 151-156. Go to original source... Go to PubMed...
    8. Doğmuº-Lehtijärvi H.T., Lehtijärvi A., Karaca G., Aday A.G. (2007): Sphaeropsis sapinea Dyko & Sutton associated with shoot blight on Brutian pine in southwestern Turkey. Acta Silvatica & Lignaria Hungarica, Special Edition (2007): 95-99. Go to original source...
    9. Eldridge K.G. (1961): Significance of Diplodia pinea in plantations. Review of Applied Mycology, 41: 339.
    10. Günay İ. (2001): Çamlarda Diplodia (Diplodia pinea Desm) Doğu Akdeniz Ormancilik Araºtirma Enstitüsü Yayinlari, 7: 12.
    11. Hantula J., Dusabenyagasani M., Hamelin R.C. (1996): Random amplified microsatellites (RAMS) - a novel method for characterizing genetic variation within fungi. European Journal of Forest Pathology, 26: 159-166. Go to original source...
    12. Maresi G., Luchi N., Pinzani P., Pazzagli M., Capretti P. (2007): Detection of Diplodia pinea in asymptomatic pine shoots and its relation to the Normalized Insolation index. Forest Pathology, 37: 272-280. Go to original source...
    13. Nicholls T.H., Ostry M.E. (1990): Sphaeropsis sapinea cankers on stressed red and jack pines in Minnesota and Wisconsin. Plant Disease, 74: 54-56. Go to original source...
    14. Palmer M.A., Nicholls T.H. (1985): Shoot blight and collar rot of Pinus resinosa caused by Sphaeropsis sapinea in forest tree nurseries. Plant Disease, 69: 739-740. Go to original source...
    15. Peterson G.W. (1977): Infection, epidemiology and control of Diplodia blight of Austrian, Ponderosa and Scots pines. Phytopathology, 67: 511-514. Go to original source...
    16. Rees A.A., Webber J.F. (1988): Pathogenicity of Sphaeropsis sapinea to seed, seedlings and saplings of some Central American pines. Transactions of the British Mycological Society, 91: 273-277. Go to original source...
    17. Sinclair W.A., Lyon H.H. (1987): Disease of Trees and Shrubs. Department of Plant Pathology. Cornell University Press, Ithaca, London: 136-137.
    18. Smith D.R., Stanosz G.R. (1995): Confirmation of two distinct populations of Sphaeropsis sapinea in the north central United States using RAPDs. Phytopathology, 85: 699-704. Go to original source...
    19. Smith D.R., Stanosz G.R. (2006): A species-specific PCR assay for detection of Diplodia pinea and D. scrobiculata in dead red and jack pines with collar rot symptoms. Plant Disease, 90: 307-313. Go to original source... Go to PubMed...
    20. Stanosz G.R., Cummings-Carlson J. (1996): Association of mortality of recently planted seedlings and established saplings in red pine plantations with Sphaeropsis collar rot. Plant Disease, 80: 750-753. Go to original source...
    21. Stanosz G.R., Smith D.R., Guthmiller M.A., Stanosz J.C. (1997): Persistence of Sphaeropsis sapinea on or in asymptomatic shoots of red and jack pine. Mycologia, 89: 525-530. Go to original source...
    22. Stanosz G.R., Swart W.J. Smith D.R. (1999): RAPD marker and isozyme characterization of Sphaeropsis sapinea from diverse coniferous hosts and locations. Mycological Research, 103: 1193-1202. Go to original source...
    23. Stanosz G.R., Blodgett J.T., Smith D.R., Kruger E.L. (2001): Water stress and Sphaeropsis as a latent pathogen of red pine seedlings. New Phytologist, 149: 531-538. Go to original source... Go to PubMed...
    24. Swart W.J., Wingfield M.J. (1991): Biology and control of Sphaeropsis sapinea on Pinus species in South Africa. Plant Disease, 75: 761-766. Go to original source...
    25. Swart W.J., Wingfield M.J., Knox-Davies P.S. (1987): Conidial dispersal of Sphaeropsis sapinea in three climatic regions of South Africa. Plant Disease, 71: 1038-1040. Go to original source...
    26. Wingfield M.J., Know-Davies P.S. (1980): Observation on diseases in pine and eucalyptus plantations in South Africa. Phytophylactica, 12: 57-63.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content