Plant Protect. Sci., 2015, 51(1):1-5 | DOI: 10.17221/94/2013-PPS

Development of a system for testing apple resistance to Erwinia amylovora using in vitro culture techniquesOriginal Paper

Jiří SEDLÁK1, František PAPRŠTEIN1, Josef KORBA2, Jana ŠILLEROVÁ2
1 Research and Breeding Institute of Pomology Holovousy Ltd., Hořice, Czech Republic
2 Division of Crop Protection and Plant Health, Department of Phytobacteriology, Crop Research Institute, Prague, Czech Republic

The suitability of different in vitro artificial inoculation methods for testing apple resistance to fire blight was evaluated. Six proliferation MS media containing BAP, TDZ or 2iP were tested to determine favourable conditions for shoot development. Out of the tested cytokinins, only media with BAP proved to be useful for all tested cultivars for artificial inoculation with Erwinia amylovora. Out of three tested inoculation methods, removal of 3 mm of the shoot apex with scissors and transfer of bacterial suspension by pipette (method 1) appeared to be the most suitable technique. This method enabled the determination of susceptibility of two cultivars due to the different speed of fire blight symptom development.

Keywords: fire blight; Malus L.; cultivar; artificial inoculation; medium; growth regulator

Published: March 31, 2015  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
SEDLÁK J, PAPRŠTEIN F, KORBA J, ŠILLEROVÁ J. Development of a system for testing apple resistance to Erwinia amylovora using in vitro culture techniques. Plant Protect. Sci. 2015;51(1):1-5. doi: 10.17221/94/2013-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abdollahi H., Rugini E., Ruzzi M., Muleo R. (2004): In vitro system for studying the interaction between Erwinia amylovora and genotypes of pear. Plant Cell, Tissue and Organ Culture, 79: 203-212. Go to original source...
    2. Billing E. (2011): Fire blight. Why do views on host invasion by Erwinia amylovora differ? Plant Pathology, 60: 178-189. Go to original source...
    3. Blažek J. (1999): Hodnocení citlivosti odrůd a genotypů hrušní po přirozené infekci spálou růžovitých (Erwinia amylovora). Vědecké práce ovocnářské, 16: 91-101.
    4. Bommineni V.R., Mathews H., Samuel S.B., Kramer M., Wagner D.R. (2001): A new method for rapid in vitro propagation of apple and pear. HortScience, 36: 1102-1106. Go to original source...
    5. Hanke V., Geider K. (2002): A new approach to evaluate fire blight resistance in vitro. Acta Horticulturae (ISHS), 590: 397-399. doi: 10.17221/94/2013-PPS Go to original source...
    6. Hevesi M., Végh A., Tóth M., Benczur E., Palkovics L. (2011): Investigating the virulence of Erwinia amylovora isolates by using apple tissue culture and pear fruit. Acta Horticulturae (ISHS), 896: 223-230. Go to original source...
    7. Jafarkhani Kermani M., Hosseini Z.S., Habashi A.A. (2009): A refined tissue culture medium for in vitro proliferation of apple rootstocks. Acta Horticulturae (ISHS), 829: 313-318. Go to original source...
    8. Kokošková B., Marhulová G., Zouhar M. (2010): New test of patogenity for the causal agent of fire blight. Zahradnictvi, 7: 51-53.
    9. Korba J., Šillerová M. (2010): Bakteriální spála ružovitých rostlin. Zahradnictví, 6: 11-13.
    10. Korba J., Šillerová M. (2011): First occurrence of fire blight on apricot (Prunus armeniaca) in Czech Republic. Acta Horticulturae (ISHS), 896: 289-292. Go to original source...
    11. Momol M.T., Aldwinkle H.S. (2000): Genetic diversity and host range of Erwinia amylovora. In: Vanneste J.L. (ed.): Fire Blight: The Disease and its Causative Agent Erwinia amylovora. Wallingford, CABI Publishing: 55-72. Go to original source...
    12. Murashige T., Skoog F. (1962): A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497. Go to original source...
    13. Murthy B.N.S., Murch S.J., Saxena Praveen K. (1998): Thidiazuron: A potent regulator of in vitro plant morphogenesis. In Vitro Cellular & Developmental Biology - Plant, 34: 267-275. Go to original source...
    14. Paprštein F., Patzák J. (2007): Spála růžovitých u jádrovin a molekulární genetika. Holovousy, Výzkumný a šlechtitelský ústav ovocnářský Holovousy s.r.o..
    15. Sarwar M, Skirvin M.R., Kushad M., Norton M.A. (1998): Selecting dwarf apple (Malus domestica Borkh.) trees in vitro: multiple cytokinin tolerance expressed among three strains of 'McIntosh' that differ in their growth habit under field conditions. Plant Cell Tissue and Organ Culture, 54: 71-76. Go to original source...
    16. Sedlák J., Paprštein F. (2008): In vitro shoot proliferation of sweet cherry cultivars Karesova and Rivan. Horticultural Science, 35: 95-98. Go to original source...
    17. Sedlák J., Paprštein F. (2009): In vitro proliferation of newly bred Czech pear cultivars. Acta Horticulturae (ISHS), 839: 87-92. Go to original source...
    18. Zwet van der T., Beer S.V. (1995): Fire Blight - Its Nature, Prevention, and Control: A practical guide to integrated disease management. Agriculture Information Bulletin No. 631. Washington, United States Department of Agriculture.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content