Plant Protection Science, 2015 (vol. 51), issue 1
Development of a system for testing apple resistance to Erwinia amylovora using in vitro culture techniquesOriginal Paper
Jiří SEDLÁK, František PAPRŠTEIN, Josef KORBA, Jana ŠILLEROVÁ
Plant Protect. Sci., 2015, 51(1):1-5 | DOI: 10.17221/94/2013-PPS 
The suitability of different in vitro artificial inoculation methods for testing apple resistance to fire blight was evaluated. Six proliferation MS media containing BAP, TDZ or 2iP were tested to determine favourable conditions for shoot development. Out of the tested cytokinins, only media with BAP proved to be useful for all tested cultivars for artificial inoculation with Erwinia amylovora. Out of three tested inoculation methods, removal of 3 mm of the shoot apex with scissors and transfer of bacterial suspension by pipette (method 1) appeared to be the most suitable technique. This method enabled the determination of susceptibility...
Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorumOriginal Paper
Tara SOLGI, Mehdi MORADYAR, Mohammad Reza ZAMANI, Mostafa MOTALLEBI
Plant Protect. Sci., 2015, 51(1):6-12 | DOI: 10.17221/83/2013-PPS
An endochitinase gene (chit33-cDNA) from the biocontrol fungus Trichoderma atroviride was overexpressed under the CaMV35S constitutive promoter in canola (R line Hyola 308). Transformation of cotyledonary petioles was achieved via Agrobacterium tumefaciens. The insertion of the transgene was verified by PCR and Southern blotting. The transgenic over-expression approach was used in order to investigate antifungal activity of expressed Chit33 on Sclerotinia sclerotiorum. Antifungal activity was detected in transgenic canola using detached leaf assay. Lesion sizes of transgenic canola caused by S. sclerotiorum...
Changes in Meligethesaeneus (Coleoptera: Nitidulidae) susceptibility to lambda-cyhalothrin in the Czech Republic between 2009 and 2011Original Paper
Marek SEIDENGLANZ, Jana POSLUŠNÁ, Jiří ROTREKL, Pavel KOLAŘÍK, Eva HRUDOVÁ, Pavel TÓTH, Jiří HAVEL, Tomáš SPITZER, Milena BERNARDOVÁ
Plant Protect. Sci., 2015, 51(1):13-32 | DOI: 10.17221/34/2014-PPS
Susceptibility of Meligethes sp. (mainly M. aeneus) from the Czech Republic was tested with lambda-cyhalothrin (111 samples in 2009, 125 in 2010, and 102 in 2011) through the use of IRAC method No. 011, Vers. 3. Resistant samples were the most frequent in all three years and their proportions increased from 33% to 62% between 2009 and 2011. The last samples classified as highly susceptible were recorded in 2010. The mean percentage mortalities for a common European field rate of 7.5 g a.i/ha were 82.2% in 2009, 78.92% in 2010, and 67.45% in 2011(according to Abbott's formula). The values of LC50 ranged from 0.084 to 47.27...
Efficacy and limitations of phosphine "spot-fumigation" against five Coleoptera species of stored product pests in wheat in a grain store - short noteShort Communication
Radek AULICKY, Vaclav STEJSKAL
Plant Protect. Sci., 2015, 51(1):33-38 | DOI: 10.17221/71/2014-PPS
Field validation of spot-fumigation with phosphine (PH3) applied by a subcontracted pest-control company in a farm grain store infested by pests was conducted. Inside and outside of the fumigated grain spot, containers with adults of 5 species of coleopteran stored-product pests were regularly spaced. The beetle pests were the internally feeding Sitophilus granarius and Rhyzopertha dominica, and the externally feeding Tribolium castaneum, Oryzaephilus surinamensis, and Cryptolestes ferrugineus. A 100% mortality of all pest species inside the fumigated spot under the sheet used for the application was stated....
Laboratory evaluation of the effect of the entomopathogenic fungi, Hirsutella thompsonii and Paecilomyces fumosoroseus, against the citrus brown mite, Eutetranychus orientalis (Acari: TetranychidaeOriginal Paper
Hamdy Mahmoud EL-SHARABASY
Plant Protect. Sci., 2015, 51(1):39-45 | DOI: 10.17221/72/2014-PPS
Formulations of the entomopathogenic fungi Hirsutella thompsonii (Fisher) and Paecilomyces fumosoroseus (Wize) Brown and Smith were tested against all stages of this pest under laboratory conditions. Three concentrations: 0.5 × 109, 1.0 × 109, and 2.0 × 109 conidia/ml were sprayed on leaf discs containing larvae, nymphs, and adults, while a single dosage of 1.0 × 109 conidia/ml wassprayed on eggs as ovicide. All the life stages were susceptible to both fungal pathogens at the tested concentrations. Larval and nymphal stages were generally less susceptible than adults. Based on probit analysis,...
Efficacy of Bacillus thuringiensis Cry14 toxin against root knot nematode, Meloidogyne javanica Original Paper
Sareh BAGHAEE RAVARI, Esmat MAHDIKHANI MOGHADDAM
Plant Protect. Sci., 2015, 51(1):46-51 | DOI: 10.17221/93/2013-PPS
Two Bacillus thuringiensis strains including ToIr65 and ToIr67 with nematicidal activity against hatched juveniles and eggs of Meloidogyne javanica were identified by phenotypic, microscopic, 16s rDNA sequencing and nematode cry gene specific PCR. Two forms of bacterial isolates including bacterial suspension (BS) and spore/crystal mixture (SCM) were tested in lab and pot conditions to evaluate their efficacy in M. javanica management. The BS of ToIr65 and ToIr67 showed 70% nematicidal activity in comparison to SCM in vitro. In pot experiments, two forms of ToIr65 significantly (by 51%) decreased number...