Plant Protect. Sci., 2015, 51(1):6-12 | DOI: 10.17221/83/2013-PPS

Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorumOriginal Paper

Tara SOLGI, Mehdi MORADYAR, Mohammad Reza ZAMANI, Mostafa MOTALLEBI
National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

An endochitinase gene (chit33-cDNA) from the biocontrol fungus Trichoderma atroviride was overexpressed under the CaMV35S constitutive promoter in canola (R line Hyola 308). Transformation of cotyledonary petioles was achieved via Agrobacterium tumefaciens. The insertion of the transgene was verified by PCR and Southern blotting. The transgenic over-expression approach was used in order to investigate antifungal activity of expressed Chit33 on Sclerotinia sclerotiorum. Antifungal activity was detected in transgenic canola using detached leaf assay. Lesion sizes of transgenic canola caused by S. sclerotiorum were significantly retarded when compared to non-transgenic canola plant.

Keywords: antifungal activity; Brassica napus; chit33-cDNA; fungal diseases

Published: March 31, 2015  Show citation

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SOLGI T, MORADYAR M, ZAMANI MR, MOTALLEBI M. Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorum. Plant Protect. Sci. 2015;51(1):6-12. doi: 10.17221/83/2013-PPS.
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