Plant Protect. Sci., 2017, 53(2):85-95 | DOI: 10.17221/59/2016-PPS

Fusarium oxysporum (F221-B) as biocontrol agent against plant pathogenic fungi in vitro and in hydroponicsOriginal Paper

Titi Thongkamngam, Tanimnun Jaenaksorn
Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand

Efficacy of non-pathogenic Fusarium oxysporum (F221-B) was assessed as a possible biocontrol agent against fungal pathogens, namely Curvularia lunata (C11, C12), F. semitectum (F113), F. oxysporum f.sp. lactucae (F221-R, F442-G), Rhizoctonia solani (R11, R12), Rhizoctonia sp. (R111, R112, R113) in vitro, while F221-B showed a moderate ability to inhibit the mycelial growth of tested fungi about 36-56%. Then, F221-B was further evaluated for its ability of controlling lettuce root rot and wilt caused by F442-G in hydroponics. It was revealed that F221-B reduced disease incidence and severity about 60-80% compared to the inoculated control and significantly promoted the growth of 3 lettuce varieties. Interestingly, using only F221-B gave the significantly highest fresh weight (twice over the healthy control). Conclusively, this study provides an important suggestion for further development of F221-B since it showed the ability of biocontrol agent and plant growth promoting fungus.

Keywords: Curvularia lunata (C11, C12); Fusarium semitectum (F113); F. oxysporum f.sp. lactucae (F221-R, F442-G); Rhizoctonia sp. (R111, R112, R113); R. solani (R11, R12); dual-culture test

Published: June 30, 2017  Show citation

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Thongkamngam T, Jaenaksorn T. Fusarium oxysporum (F221-B) as biocontrol agent against plant pathogenic fungi in vitro and in hydroponics. Plant Protect. Sci. 2017;53(2):85-95. doi: 10.17221/59/2016-PPS.
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    References

    1. Abeysinghe S. (2006): Biological control of Fusarium oxysporum f.sp. radicis-cucumerinum, the causal agent of root and stem rot of Cucumis sativus by non-pathogenic Fusarium oxysporum. Ruhuna Journal of Science, 1: 24-31. Go to original source...
    2. Alabouvette C. (1990): Biological control of Fusarium wilts pathogen in suppressive soil. In: Hornby D. (ed.): Biological Control of Soil-borne Plant Pathogens. Wallingford, CABI Publishing: 27-43.
    3. Alabouvette C., Couteaudier Y. (1992): Biological control of Fusarium wilts with nonpathogenic Fusaria. In: Tjamos E.C., Pavizas G.C., Cook R.J. (eds): Biological Control of Plant Diseases. New York, Springer-US Plenum Press: 415-426. Go to original source...
    4. Alabouvette C., Lemanceau P., Steinberg C. (1993): Recent advance in biological control of Fusarium wilt. Pesticide Science, 37: 365-373. Go to original source...
    5. Alabouvette C., Lemanceau P., Steinberg C. (1996): Use of nonpathogenic Fusarium oxysporum and fluorescent pseudomonads to control Fusarium wilts. In: Tang W., Cook R.J., Rovira A.D. (eds): Advances in Biological Control of Plant Diseases. Bejing, China Agricultural University Press: 155-164.
    6. Alabouvette C., Steinberg C., Lemanceau P., Bakker P.A.H.M. (1998): Biological control of Fusarium wilt towards the development of commercial products. In: Boland G.J., Kuykendall L.D. (eds): Plant Microbe Interaction and Biological Control. New York, Marcel Dekker: 15-36.
    7. Benhamou N., Charest P.M., Jarvis W.R. (1989): Biology and host-parasite relationships of Fusarium oxysporum. In: Tjamos E.C., Beckman C.H. (eds): Vascular Wilt Disease of Plant. Basic Studies and Control: NATO ASI Series. Berlin Heidelberg, Springer-Verlag: 95-105. Go to original source...
    8. Benhamou N., Garand C., Goulet A. (2002): Ability of nonpathogenic Fusarium oxysporum strain Fo47 to induce resistance against Pythium ultimum infection in cucumber. Applied and Environmental Microbiology, 68: 4044-4060. Go to original source... Go to PubMed...
    9. Chen D., Liu X., Li C., Tian W., Shen Q., Shen B. (2014): Isolation of Bacillus amyloliquefaciens S20 and its application in control of eggplant bacterial wilt. Journal of Environmental Management, 137: 120-127. Go to original source... Go to PubMed...
    10. Dennis C., Webster J. (1971): Antagonistic properties of species groups of Trichoderma-III. Hyphal interaction. Transactions of the British Mycological Society, 51: 363-369. Go to original source...
    11. Duijff B.J., Pouhair D., Olivain C., Alabouvette C., Lemanceau P. (1998): Implication of systemic induced resistance in the suppression of Fusarium wilt of tomato by Pseudomonas fluorescent WSC417r and by non-pathogenic Fusarium oxysporum Fo47. European Journal of Plant Pathology, 104: 903-910. Go to original source...
    12. Duijff B.J., Recorber G., Bakker P.A.H.M., Loper J.E., Lemanceau P. (1999): Microbial antagonism at the root level is involved in the suppression of Fusarium wilt by the combination of non-pathogenic Fusarium oxysporum Fo47 and Pseudomonas putida WCS358. Phytopathology, 89: 1073-1079. Go to original source... Go to PubMed...
    13. Fravel D., Olivain C., Alabouvette C. (2003): Fusarium oxysporum and its biocontrol. New Phytologist, 15: 493-502. Go to original source... Go to PubMed...
    14. Fuchs J.G., Moenne-Loccoz Y., Defago G. (1997): Nonpathogenic Fusarium oxysporum strain Fo47 induces resistance to Fusarium wilt of tomato. Plant Disease, 81: 492-496. Go to original source... Go to PubMed...
    15. Fuchs J.G., Moenne-Loccoz Y., Defago G. (1999): Ability of nonpathogenic Fusarium oxysporum Fo47 to protect tomato against Fusarium wilt. Biological Control, 14: 105-110. Go to original source...
    16. Ghini R., Mezzalama M., Ambrosoli R., Barberis E., Garibaldi A., Piedade S.M.S. (2000): Fusarium oxysporum strains as biocontrol agents against Fusarium wilt: effects on soil microbial biomass and activity. Pesquisa Agropecuaria Brasileira, 35: 93-101. Go to original source...
    17. Hervas A., Landa B., Datnoff L.E., Jimenez-Diaz R.M. (1998): Effects of commercial and indigenous microorganisms on Fusarium wilt development in chickpea. Biological Control, 13: 166-176. Go to original source...
    18. Honda M., Kawakub Y. (1998): Control of Fusarium basal rot of rakkyo (Allium chinense) nonpathogenic Fusarium moniliforme and Fusarium oxysporum. Soil Microorganisms, 51: 13-18.
    19. Horinouchi H., Katsuyama N., Taguchi Y., Hyakumachi M. (2008): Control of Fusarium crown and root rot of tomato in a soil system by combination of a plant growthpromoting fungus, Fusarium equiseti, and biodegradable pots. Crop Protection, 27: 859-864. Go to original source...
    20. Horinouchi H., Muslim A., Hyakumachi M. (2010): Biocontrol of Fusarium wilt of spinach by the plant growth promoting fungus Fusarium equiseti GF183. Journal of Plant Pathology, 92: 251-256.
    21. Howell C.R. (2003): Mechanisms employed by Trichoderma species in the biological control of plant disease: The history and evolution of current concepts. Plant Disease, 87: 4-10. Go to original source... Go to PubMed...
    22. Kakvan N., Heydari A., Zamanizadeh H.A., Rezaee S., Naraghi L. (2013): Development of new bioformulations using Trichoderma and Talaromyces fungal antagonists for biological control of sugar beet damping-off disease. Crop Protection, 53: 80-84. Go to original source...
    23. Katsube K., Alasaka Y. (1997): Control of Fusarium wilt of spinach by transplanting seedling pretreated with nonpathogenic Fusarium oxysporum. Annals of the Phytopathological Society of Japan, 63: 389-394. Go to original source...
    24. Kaur R. (2003): Characterization of selected isolates of non-pathogenic Fusarium oxysporum, fluorescent Pseudomonads and their efficacy against chickpea wilt. [PhD Thesis.] Ludhiana, Punjab Agricultural University.
    25. Kaur R., Kaur J., Singh R.S. (2010): Nonpathogenic Fusarium as a biological control agent. Plant Pathology, 9: 79-91. Go to original source...
    26. Kim H.J., Lee E.J., Park S.H., Lee H., Chung N. (2014): Biological control of anthracnose (Colletotrichum gleosporioides) in pepper and cherry tomato by Streptomyces sp. A1022. Journal of Agricultural Science, 6: 54-62. Go to original source...
    27. Larkin R.P., Fravel D.R. (1998): Efficacy of various fungal and bacterial biocontrol organisms for control of Fusarium wilt of tomato. Plant Disease, 82: 1022-1028. Go to original source... Go to PubMed...
    28. Larkin R.P., Fravel D.R. (1999): Mechanisms of action and dose-response relationships governing biological control of Fusarium wilt of tomato by non-pathogenic Fusarium spp. Phytopathology, 89: 1152-1161. Go to original source... Go to PubMed...
    29. Larkin R.P., Hopkins D.L., Martin F.N. (1996): Suppression of Fusarium wilt of watermelon by nonpathogenic Fusarium oxysporum and other microorganisms recovered from a disease-suppressive soil. Phytopathology, 86: 812-819. Go to original source...
    30. Lemanceau P., Bakker P.A.H.M., De Kogel W.J., Alabouvette C., Schippers B. (1992): Effect of pseudobactin 358 production by Pseudomonas putida WCS 358 on suppression of Fusarium wilt of carnation by nonpathogenic Fusarium oxysporum Fo47. Applied and Environmental Microbiology, 58: 2978-2982. Go to original source... Go to PubMed...
    31. Lemanceau P., Bakker P.A.H.M., De Kogel W.J., Alabouvette C., Schippers B. (1993): Antagonistic effect of nonpathodoi: 10.17221/59/2016-PPS genic Fusarium oxysporum Fo47 and Pseudobactin 358 upon pathogenic Fusarium oxysporum f.sp. dianthi. Applied and Environmental Microbiology, 59: 74-82. Go to original source... Go to PubMed...
    32. Mandeel Q., Baker R. (1991): Mechanisms involved in biological control of Fusarium wilt of cucumber with strains of nonpathogenic Fusarium oxysporum. Phytopathology, 81: 462-468. Go to original source...
    33. Minuto A., Minuto G., Migheli Q., Mocioni M., Gullino M.L. (1997): Effect of antagonistic Fusarium spp. and of different commercial biofungicide formulations on Fusarium wilt of basil (Ocimum basilicum L.). Crop Protection, 16: 765-769. Go to original source...
    34. Minuto A., Clematis F., Gullino M.L., Garibaldi A. (2007): Induced suppressiveness to Fusarium oxysporum f.sp. radicis lycopersici in rockwool substrate used in closed soilless systems. Phytoparasitica, 35: 77-85. Go to original source...
    35. Nahalkova J., Fatehi J., Olivain C., Alabouvette C. (2008): Tomato root colonization by fluorescent-tagged pathogenic and protective strains of Fusarium oxysporum in hydroponic culture differs from root colonization in soil. Federation of European Microbiological Societies, 286: 152-157. Go to original source... Go to PubMed...
    36. Nel B., Steinberg C., Labuschagne N., Viljoen A. (2006): The potential of nonpathogenic Fusarium oxysporum and other biological control organisms for suppressing Fusarium wilt of banana. Plant Pathology, 55: 217-223. Go to original source...
    37. Olivain C., Alabouvette C. (1999): Process of tomato root colonization by a pathogenic strain of Fusarium oxysporum f.sp. lycopersici discussed in comparison to a nonpathogenic strain. New Phytologist, 141: 497-510. Go to original source...
    38. Paparu P., Dubois T., Gold C.S., Niere B., Adipala E., Coyne D. (2006): Colonisation pattern of nonpathogenic Fusarium oxysporum, a potential biological control agent, in roots and rhizomes of tissue cultured Musa plantlets. Annals of Applied Biology, 149: 1-8. Go to original source...
    39. Patil S., Sriram S., Savitha M.J. (2011): Evaluation of nonpathogenic Fusarium for antagonistic activity against Fusarium wilt of tomato. Journal of Biological Control, 25: 118-123.
    40. Paul J., Singh R.S., Kaur J., Alabouvette C. (1999): Effect of inoculum density of nonpathogenic Fusarium in biological control of chickpea wilt caused by Fusarium oxysporum f.sp. cicero. In: Biological Control Based Pest Management for Quality Crop Protection in the Current Millennium. Ludhiana, Punjab Agricultural University: 97-98.
    41. Rodriguez M.A., Cabrera G., Godeas A. (2006): Cyclosporine A from a nonpathogenic Fusarium oxysporum suppressing Sclerotinia sclerotiorum. Journal of Applied Microbiology, 100: 575-586. Go to original source... Go to PubMed...
    42. Song M., Yun H.Y., Kim Y.H. (2014): Antagonistic Bacillus species as a biological control of ginseng root rot caused by Fusarium cf. incarnatum. Journal of Ginseng Research, 38: 136-145. Go to original source... Go to PubMed...
    43. Thongkamngam T., Koohakan P., Jaenaksorn T. (2012): First report of Fusarium wilt of NFT-grown lettuce caused by Fusarium oxysporum f.sp. lactucae in Thailand and its pathogenicity on four varieties of lettuce. In: 10th Naresuan Conference, July 24-25, 2012, Phitsanulok, Thailand: 72-81. (in Thai)
    44. Thongkamngam T., Koohakan P., Jaenaksorn T. (2013): Fusarium oxysporum F221-B as plant growth-promoting fungus (PGPF) on six plants in hydroponics and its growth characteristics on different media. In: 6th Rajamangala University of Technology Tawan-ok Research Conference, Chonburi, Thailand: 46-51. (in Thai)

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