Plant Protect. Sci., 2018, 54(4):215-221 | DOI: 10.17221/158/2017-PPS

Cloning and characterisation of nanobodies against the coat protein of Zucchini yellow mosaic virusOriginal Paper

Adel M. Zakri1*, Abdullah A. Al-Doss1, Markus Sack2, Ahmed A. Ali1, Emad M. Samara3, Basem S. Ahmed1, Mahmoud. A. Amer4, Omar. A. Abdalla4, Mohammed A. Al-Saleh4
1 Department of Plant Production, 3Department of Animal Production and 4Department of Plant Protection, College of Food &
2 Agriculture Science, King Saud University, Riyadh, Saudi Arabia
3 Institute for Molecular Biotechnology, RWTH Aachen University, Aachen, Germany

Zucchini yellow mosaic virus (ZYMV), in the family Potyviridae, causes an economically important disease. Antibodies are valuable reagents for diagnostic assays to rapidly detect viral infection. Here, we report the isolation of camel-derived variable domains of the heavy chain antibody (VHH, also called nanobodies) directed against the coat protein (CP) of ZYMV. Several nanobodies that specifically recognise ZYMV-CP were identified. The isolated nanobodies showed binding not only to recombinant ZYMV-CP but also to native ZYMV, indicating that these nanobodies can be used in diagnostic tools to detect viral infections.

Keywords: nanobodies; VHH; naïve library; phage display

Published: December 31, 2018  Show citation

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Zakri AM, Al-Doss AA, Sack M, Ali AA, Samara EM, Ahmed BS, et al.. Cloning and characterisation of nanobodies against the coat protein of Zucchini yellow mosaic virus. Plant Protect. Sci. 2018;54(4):215-221. doi: 10.17221/158/2017-PPS.
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