Plant Protect. Sci., 2018, 54(4):258-264 | DOI: 10.17221/118/2017-PPS

Control of plant sap-sucking insects using entomopathogenic fungi Isaria fumosorosea strain (Ifu13a)Original Paper

Ghulam Ali Bugti1,2, Cao Na1, Wang Bin*,1, Lin Hua Feng*,2
1 Provincial Key Laboratory of Microbial Control and
2 School of Plant Protection, Anhui Agricultural University, Hefei, P.R. China

The virulence of the Isaria fumosorosea strain (Ifu13a) against different plant sap-sucking insects such as Jacobiasca formosana Paoli (Hemiptera: Cicadellidae), Aphis gossypii Glover (Hemiptera: Aphididae), Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae), and Stephanitis nashi Esaki et Takeya (Hemiptera: Tingidae) in laboratory condition at 21 ± 1°C temperature and 78 ± 5% relative humidity was determined. We found that the Ifu13a strain had excellent potential to control the target insects. The mortality of the tested insect species ranged from 81 to 100% in the concentration of 1 × 108 conidia/ml. However, the lowest mortality of 33% was observed in the concentration of 1 × 105 conidia/ml against the S. nashi population. Median lethal times (LT50) were obtained from a regression-probit value which indicated 4.1, 4.1, 4.8, and 7.3 days at a concentration of 1 × 108 conidia/ml, whereas, median lethal concentration dosages (LC50) were calculated as 3.9 × 103, 6.8 × 104, 3.0 × 104, and 6.9 × 105 conidia/ml against J. formosana, A. gossypii, B. tabaci, and S. nashi, respectively. The present study showed that the Ifu13a fungal strain is highly pathogenic to the target insects, and it can be used as a biocontrol agent against plant sap-sucking insect species under favourable weather conditions.

Keywords: microbial control; entomopathogen; Jacobiasca formosana; Aphis gossypii; Bemisia tabaci; Stephanitis nashi

Published: December 31, 2018  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Bugti GA, Na C, Bin W, Feng LH. Control of plant sap-sucking insects using entomopathogenic fungi Isaria fumosorosea strain (Ifu13a). Plant Protect. Sci. 2018;54(4):258-264. doi: 10.17221/118/2017-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abbott W.S. (1925): A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265-267. Go to original source...
    2. Ali S., Huang Z., Zou S.X., Bashir M. H., Wang Z.Q., Ren S.X. (2012): The effect of insecticides on growth, germination and cuticle-degrading enzyme production by Isaria fumosorosea. Biocontrol Science and Technology, 22: 1047-1058. Go to original source...
    3. Antwi F.B., Reddy G.V. (2015): Toxicological effects of pyrethroids on non-target aquatic insects. Environmental Toxicology & Pharmacology 40: 915-923. Go to original source... Go to PubMed...
    4. Arthurs S.P., Aristizábal L.F., Avery P.B. (2013): Evaluation of entomopathogenic fungi against chilli thrips, Scirtothrips dorsalis. Journal of Insect Science, 13: 1-16. Go to original source... Go to PubMed...
    5. Baffourawuah S., Annan A.A., Maigaascofare O., Dieudonné S.D., Adjeikusi P., Owusudabo E., Obiridanso K. (2016): Insecticide resistance in malaria vectors in Kumasi, Ghana. Parasites & Vectors, 9: 633. Go to original source... Go to PubMed...
    6. Baiyun, Yuhong C., NA C., Yihao L., Bugti G.A., Bin W. (2016): Effects of Humidity and Temperature on the pathogenecity of Beauveria bassiana against Stephanitis nashi and Locusta migratoria manilensis. Chinese Journal of Biological Control, 6: 735-742.
    7. Barrios C.E., Bustillo A.E., Ocampo K.L., Reina M.A., Alvarado M.H.L. (2016): Efficacy of entomopathogenic fungi to control Leptopharsa gibbicarina (Hemiptera: Tingidae) in oil palm. Revista Colombiana de Entomologia, 42: 22-27. Go to original source...
    8. Boopathi T., Karuppuchamy P., Singh S.B., Kalyanasundaram M., Mohankumar S., Ravi M. (2015a): Microbial control of the invasive spiraling whitefly on cassava with entomopathogenic fungi. Brazilian Journal of Microbiology, 46: 1077-1085. Go to original source... Go to PubMed...
    9. Boopathi T., Karuppuchamy P., Singh S.B., Ravi M., Manju T. (2015b): Microbial control of exotic spiraling whitefly, Aleurodicus dispersus with entomopathogenic fungi on cassava under open field conditions. Indian Journal of Horticulture, 72: 370-375. Go to original source...
    10. Brück E., Elbert A., Fischer R., Krueger S., Kühnhold J., Klueken A.M., Nauen R., Niebes J.F., Reckmann U., Schnorbach H.J. (2009): Movento ®, an innovative ambimobile insecticide for sucking insect pest control in agriculture: biological profile and field performance. Crop Protection, 28: 838-844. Go to original source...
    11. Chandler D., Heale J.B., Gillespie A.T. (1994): Effect of osmotic potential on the germination of conidia and colony growth of Verticillium lecanii. Mycological Research, 98: 384-388. Go to original source...
    12. Chen M., Zhang D., Peng F., Zengzhi L.I. (2014): Wettable powder development of isaria javanica for control of the lesser green leafhopper, Empoasca vitis. Chinese Journal of Biological Control, 30: 51-57.
    13. Crop I. (2012): IBM SPSS Statistics for Windows, Version 21.0. Armonk, IBM Corp.
    14. Dhaliwal G.S., Jindal V., Dhawan A.K. (2010): Insect pest problems and crop losses: changing trends. Indian Journal of Ecology, 37: 1-7.
    15. Duarte R.T., Goncalves K.C., Espinosa D.J.L., Moreira L.F., de Bortoli S.A., Humber R.A., Polanczyk R.A. (2016): Potential of entomopathogenic fungi as biological control agents of diamondback moth (Lepidoptera: Plutellidae) and compatibility with chemical insecticides. Journal of Economic Entomology, 109: 594-601. Go to original source... Go to PubMed...
    16. Faria M., Wraight S.P. (2001): Biological control of Bemisia tabaci with fungi. Crop Protection, 20: 767-778. Go to original source...
    17. Finney D.J. (1971): Probit Analysis. New York, Cambridge University Press.
    18. Fransen J.J., Winkelman K., Lenteren J.C.V. (1987): The differential mortality at various life stages of the greenhouse whitefly, Trialeurodes vaporariorum (Homoptera: Aleyrodidae), by infection with the fungus Aschersonia aleyrodis (Deuteromycotina: Coelomycetes). Journal of Invertebrate Pathology, 50: 158-165. Go to original source...
    19. Fytrou A., Schofield P.G., Kraaijeveld A.R., Hubbard S.F. (2006): Wolbachia infection suppresses both host defence and parasitoid counter-defence. Proceedings of the Royal Society B: Biological Sciences, 273: 791-796. Go to original source... Go to PubMed...
    20. Gao T., Wang Z., Huang Y., Keyhani N.O., Huang Z. (2017): Lack of resistance development in Bemisia tabaci to Isaria fumosorosea after multiple generations of selection. Scientific Reports, 7: Article No. 42727. doi:10.1038/srep42727 Go to original source... Go to PubMed...
    21. Haider I., Suhail A. (2013): Selectivity of Insecticides to Beneficial Insect. Saarbrücken, Lap Lambert Academic Publishing.
    22. Jandricic S.E., Filotas M., Sanderson J.P., Wraight S.P. (2014): Pathogenicity of conidia-based preparations of entomopathogenic fungi against the greenhouse pest aphids Myzus persicae, Aphis gossypii, and Aulacorthum solani (Hemiptera: Aphididae). Journal of Invertebrate Pathology, 118: 34-46. Go to original source... Go to PubMed...
    23. Jaronski S.T. (2010): Ecological factors in the inundative use of fungal entomopathogens. BioControl, 55: 159-185. Go to original source...
    24. Kavallieratos N.G., Athanassiou C.G., Aountala M.M., Kontodimas D.C. (2014): Evaluation of the entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea for control of Sitophilus oryzae. Journal of Food Protection, 77: 87-93. Go to original source... Go to PubMed...
    25. Kontsedalov S., Abu-Moch F., Lebedev G., Czosnek H., Horowitz A.R., Ghanim M. (2012): Bemisia tabaci biotype dynamics and resistance to insecticides in Israel during the years 2008-2010. Journal of Integrative Agriculture, 11: 312-320. Go to original source...
    26. Lacey L.A., Fransen J.J., Carruthers R.I. (1996): Global distribution of naturally occurring fungi of Bemisia, their biologies and use as biological control agents. In: Gerling D., Mayer R.T. (eds): Bemisia 1995: Taxonomy, Biology, Damage, Control and Management. Andover, Intercept: 356-456.
    27. Long D.W., Drummond F.A., Groden E., Donahue D.W. (2000): Modelling Beauveria bassiana horizontal transmission. Agricultural & Forest Entomology, 2: 19-32. Go to original source...
    28. Majeed M.Z., Ma C.S., Fiaz M., Afzal M. (2017): Entomopathogenicity of three muscardine fungi, Beauveria bassiana, Isaria fumosorosea and Metarhizium anisopliae, against the Asian citrus psyllid, Diaphorina citri kuwayama (Hemiptera: Psyllidae). Egyptian Journal of Biological Pest Control, 27: 211-216.
    29. Müller E.J. (2000): Control of the brown locust, Locustana pardalina (Walker) (Orthoptera: Acrididae), using a mycoinsecticide: addressing the issues of speed-of-kill, dose rate, mortality and reduction in feeding. African Entomology, 8: 217-221.
    30. Muñiz-Paredes F., Miranda-Hernández F., Loera O. (2017): Production of conidia by entomopathogenic fungi: from inoculants to final quality tests. World Journal of Microbiology and Biotechnology, 33: 57. Go to original source... Go to PubMed...
    31. Potrich M., Neves P.M.O.J., Alves L.F.A., Pizzatto M., da Silva E.R.L., Luckmann D., de Gouvea A., Roman J.C. (2011): Virulência de fungos entomopatogênicos a ninfas de Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae). Semina-Ciencias Agrarias, 32: 1783-1791. Go to original source...
    32. Robert S., Petra C., Juraj M., Marek B. (2016): Natural prevalence of entomopathogenic fungi in hibernating pupae of Cameraria ohridella (Lepidoptera: Gracillariidae) and virulence of selected isolates. Plant Protection Science, 52: 199-208. Go to original source...
    33. Salim-Abadi Y., Oshaghi M.A., Enayati A.A., Abai M.R., Vatandoost H., Eshraghian M.R., Mirhendi H., HanafiBojd A.A., Gorouhi M.A., Rafi F. (2016): High insecticides resistance in Culex pipiens (Diptera: Culicidae) from Tehran, capital of Iran. Journal of Arthropod-Borne Diseases, 10: 483-492. Go to PubMed...
    34. Sevim A., Donzelli B.G., Wu D., Demirbag Z., Gibson D.M., Turgeon B.G. (2012): Hydrophobin genes of the entomopathogenic fungus, Metarhizium brunneum, are differentially expressed and corresponding mutants are decreased in virulence. Current Genetics, 58: 79-92. Go to original source... Go to PubMed...
    35. Sevim A., Demir I., Sonmez E., Kocacevik S., Demirbag Z. (2013): Evaluation of entomopathogenic fungi against the sycamore lace bug, Corythucha ciliata (Say) (Hemiptera: Tingidae). Turkish Journal of Agriculture and Forestry, 37: 595-603. Go to original source...
    36. Sosa-Gómez D.R., Alves S.B. (2000): Temperature and relative humidity requirements for conidiogenesis of Beauveria bassiana (Deuteromycetes: Moniliaceae). Anais da Sociedade Entomológica do Brasil, 29: 515-521. Go to original source...
    37. Shang Y., Feng P., Wang C. (2015): Fungi that infect insects: Altering host behavior and beyond. PLoS Pathogens, 11 (8): e1005037. doi: 10.1371/journal.ppat.1005037 Go to original source... Go to PubMed...
    38. Zimmermann G. (2008): The entomopathogenic fungi Isaria farinosa (formerly Paecilomyces farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus): biology, ecology and use in biological control. Biocontrol Science and Technology, 18: 865-901. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content