Plant Protect. Sci., 2019, 55(2):81-89 | DOI: 10.17221/42/2018-PPS

Wheat dwarf virus infectious clones allow to infect wheat and Triticum monococcum plantsOriginal Paper

Pavel Cejnar1, Ludmila Ohnoutková2, Jan Ripl1, Jiban Kumar Kundu*,1
1 Division of Crop Protection and Plant Health, Crop Research Institute, Prague, Czech Republic
2 Department of Chemical Biology and Genetics, Centre of the Hana Region for Biotechnological and Agricultural Research, Palacky University Olomouc, Olomouc, Czech Republic

We constructed Wheat dwarf virus (WDV) infectious clones in the bacterial plasmids pUC18 and pIPKb002 and tested their ability to inoculate plants using Bio-Rad Helios Gene Gun biolistic inoculation method and Agrobacterium tumefaciens agroinoculation method, and we then compared them with the natural inoculation method via viruliferous P. alienus. Infected plants were generated using both infectious clones, whereas the agroinoculation method was able to produce strong systemic infection in all three tested cultivars of wheat and Triticum monococcum, comparable to plants inoculated by viruliferous P. alienus. Infection was confirmed by DAS-ELISA, and WDV titres were quantified using qPCR. The levels of remaining bacterial plasmid DNA were also confirmed to be zero.

Keywords: WDV; Triticum aestivum L.; virus infectious clone; agroinoculation; biolistic inoculation; leafhopper; qPCR detection

Published: June 30, 2019  Show citation

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Cejnar P, Ohnoutková L, Ripl J, Kundu JK. Wheat dwarf virus infectious clones allow to infect wheat and Triticum monococcum plants. Plant Protect. Sci. 2019;55(2):81-89. doi: 10.17221/42/2018-PPS.
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