Plant Protect. Sci., 2019, 55(3):172-180 | DOI: 10.17221/21/2018-PPS

Fusarium culmorum Tri genes and barley Hvugt13248 gene transcription in infected barley cultivarsOriginal Paper

Zuzana Faltusová1, Kateřina Vaculová2, Jozef Pavel1, Ilona Svobodová2, Jana Hajšlová3, Jaroslava Ovesná1*
1 Crop Research Institute, Prague, Czech Republic
2 Agrotest Fyto, Ltd., Kroměříž, Czech Republic
3 Institute of Chemical Technology Prague, Prague, Czech Republic

The transcription activities of genes somehow associated with the mycotoxin deoxynivalenol (DON) biosynthesis, namely Fusarium Tri genes, and the barley gene coding for UDP-glycosyltransferase (HvUGT13248) on different genetic backgrounds were compared. Determining the amount of the pathogen DNA was used as a useful tool for evaluating the infestation of barley cultivars. Amounts of the pathogen DNA differed in six barley cultivars infected by F. culmorum. Transcription of HvUGT13248 was related to DON content in the samples. Low pathogenic infection and low DON content were accompanied by increased Fusarium Tri10 transcription in resistant cv. Amulet. This finding confirmed our recent results and makes us propose using this change as a possible marker of barley resistance against Fusarium.

Keywords: barley; UDP-glycosyltransferase; RT qPCR; DON biosynthesis

Published: September 30, 2019  Show citation

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Faltusová Z, Vaculová K, Pavel J, Svobodová I, Hajšlová J, Ovesná J. Fusarium culmorum Tri genes and barley Hvugt13248 gene transcription in infected barley cultivars. Plant Protect. Sci. 2019;55(3):172-180. doi: 10.17221/21/2018-PPS.
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