Plant Protect. Sci., 2020, 56(3):180-190 | DOI: 10.17221/41/2019-PPS

Antifungal activity of microbial nanoparticles derived from Chaetomium spp. against Magnaporthe oryzae causing rice blastOriginal Paper

Jiao Jiao Song*,1, Kasem Soytong1, Somdej Kanokmedhakul2, Kwanjai Kanokmedhakul2, Supattra Poeaim3
1 Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Ladkrabang, Bangkok, Thailand
2 Natural Products Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand
3 Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Ladkrabang, Bangkok, Thailand

The Magnaporthe oryzae isolate PO2 was proven to cause rice blast var. PSL 2 in this study. Chaetomium elatum, Chaetomium lucknowense and Chaetomium brasiliense were antagonised to M. oryzae. The hexane extract of C. brasiliense gave the highest inhibition of the spore production with an ED50 of 35 ppm, the EtOAC extract of C. lucknowense inhibited the spore production at 57 ppm and the EtOAC extracts of C. elatum inhibited the spore production at 106 ppm. The nano-CLM (C. lucknowense) inhibited the spore production at 5.24 ppm, the nano-CBH (C. brasiliense) inhibited the spore production at 6.86 ppm and the nano-CEE (C. elatum) inhibited the spore production at 7.89 ppm. The rice leaves treated with nano-CBH from C. brasiliense produced Sakuranertin and Oryzalexin B as seen on the thin layer chromatography where the Rf value was 0.08 assumed to be Sakuranertin, and the Rf value of 0.28 supposed to be Oryzalexin B. It was found that the nanoparticles act as elicitors to induce immunity in rice plants through the production of phytoalexin.

Keywords: plant pathology; biological control; antagonistic fungi; blast disease

Published: September 30, 2020  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Song JJ, Soytong K, Kanokmedhakul S, Kanokmedhakul K, Poeaim S. Antifungal activity of microbial nanoparticles derived from Chaetomium spp. against Magnaporthe oryzae causing rice blast. Plant Protect. Sci. 2020;56(3):180-190. doi: 10.17221/41/2019-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Akatsuka T., Kodama O., Kato H., Kono Y., Takeuchi S. (1983): 3-Hydroxy-7-oxo-sandaraco- pimaradiene (Oryzalexin A), a new phytoalexin isolated from rice blast leaves. Agricultural and Biological Chemistry, 77: 1141-1148. Go to original source...
    2. Altschul S.F., Madden T.L., Schaffer A.A., Zhang J.H., Zhang Z., Miller W., Lipman D.J. (1997): Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25: 3389-3402. Go to original source... Go to PubMed...
    3. Dar J., Soytong K. (2014): Construction and characterization of copolymer nanomaterials loaded with bioactive compounds from Chaetomium species. Journal of Agricultural Technology, 10: 823-831.
    4. Devi S.J.S.R., Singh K., Umakanth B., Vishalakshi B., Renuka P., Sudhakar K.V., Prasad M. S., Viraktamath B.C., Babu V.R., Madhav M.S. (2015): Development and identification of novel rice blast resistant sources and their characterization using molecular markers. Rice Science, 22: 300-308. Go to original source...
    5. Dillon V.M., Overton J., Grayer R.J., Harbone J. (1997): Differences in phytoalexin response among rice cultivars of different resistance to blast. Phytochemistry, 44: 559-603. Go to original source...
    6. Elibol O.H., Morisette D.D., Denton J.P., Bashir R. (2003): Integrated nanoscale silicon sensors using top-down fabrication. Applied Physics Letters, 83: 4613-4615. Go to original source...
    7. FAO (2009): FAO's director-general on how to feed the world in 2050. Insights from an Expert Meeting at FAO, 1: 1-35.
    8. Hasegawa M., Mitsuhara I., Seo S., Okada K., Yamane H., Iwai T., Ohashi Y. (2014): Analysis on blast fungus-responsive characters of a flavonoid phytoalexin sakuranetin; Accumulation in infected rice leaves, antifungal activity and detoxification by fungus. Molecules, 19: 11404-11418. Go to original source... Go to PubMed...
    9. Jagadeesh D., Prasanna Kumar M.K., Chandrakanth R., Devaki N.S. (2018): Molecular diversity of internal transcribed spacer among the monoconidial isolates of Magnaporthe oryzae isolated from rice in Southern Karnataka, India. Journal of Genetic Engineering and Biotechnology, 16: 631-638. Go to original source... Go to PubMed...
    10. Khumkomkhet P., Kanokmedhakul S., Kanokmedhakul K., Hahnvajanawong C., Soytong K. (2009): Antimalarial and cytotoxic depsidones from the fungus Chaetomium brasiliense. Journal of Natural Products, 72: 1 487-1491. Go to original source... Go to PubMed...
    11. Kodama O., Miyakawa J., Akatsuka T., Kiyosawa S. (1992): Sakuranetin, a flavanone phytoalexin from ultravioletirradiated rice leaves. Phytochemistry, 31: 3807-3809. Go to original source...
    12. Li M., Huang Q., Wu Y. (2011): A novel chitosan-poly (lactide) copolymer and its submicron particles as imidacloprid carriers. Pest Management Science, 67: 831-836. Go to original source... Go to PubMed...
    13. Miah G., Rafill M.Y., Ismail M.R., Puteh A.B., Rahim A.B., Asfaliza R., Latif M.A. (2013): Blast resistance in rice: a review of conventional breeding to molecular approaches. Molecular Biology Reports, 40: 2369-2388. Go to original source... Go to PubMed...
    14. Ou S.H. (1985): Rice Diseases. UK, Common Wealth Mycological Institute.
    15. Pornsuriya C., Soytong K., Poeaim S., Kanokmedhakul S., Khumkomkhet P., Lin F.C., Wang H.K., Hyde K.D. (2011): Chaetomium siamense sp. nov., a soil isolate from Thailand, produces a new chaetoviridin, G. Mycotaxon, 115: 17-19. Go to original source...
    16. Rai M., Ingle A. (2012): Role of nanotechnology in agriculture with special reference to management of insect-pest. Applied Microbiology and Biotechnology, 94: 287-293. Go to original source... Go to PubMed...
    17. Sibounnavong P., Kanokmedhakul S., Soytong K. (2011): Antifungal metabolites from antagonistic fungi used to control tomato wilt fungus Fusarium oxysporum f. sp. lycopersici. African Journal of Biotechnology, 10: 19714-19722. Go to original source...
    18. Song J.J., Soytong K. (2016): Antifungal activity of Chaetomium elatum against Pyricularia oryze causing rice blast. International Journal of Agricultural Technology, 12: 1437-1447.
    19. Song J.J., Soytong K. (2016): Antifungal activity of Chaetomium elatum against Pyricularia oryze causing rice blast. Journal of Agricultural Technology, 12: 1437-1447.
    20. Song J.J., Soytong K., Kanokmedhakul S. (2017): Fungal metabolites of Chaetomium lucknowense for inhibition of a rice blast pathogen, Pyricularia oryzae. International Journal of Agricultural Technology, 13: 1621-1626.
    21. Soutter W. (2013): Nanotechnology in agriculture. Available at http://www.azonano.com/article.aspx?ArticleID=3141#1%204 (accessed May 25, 2013).
    22. Soytong K. (1992): Antagonism of Chaetomium cupreum to Pyricularia oryzae. Journal of Plant Protection in the Tropics, 9: 17-23.
    23. Soytong K. (2014): Bio-formulation of Chaetomium cochliodes for controlling brown leaf spot of rice. Journal of Agricultural Technology, 10: 321-337.
    24. Soytong K., Charoenporn C., Kanokmedhakul S. (2013): Evaluation of microbial elicitors to induce plant immunity for tomato wilt. African Journal of Microbiology Research, 7: 1993-2000. Go to original source...
    25. Soytong K., Kanokmedhakul S., Kukongviriyapan V., Isobe M. (2001): Application of Chaetomium species (Ketomium) as a new broad spectrum biological fungicide for plant disease control: a review article. Fungal Diversity, 7: 1-15.
    26. Tamura K., Dudley J., Nei M., Kumar S. (2007): MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24: 1596-1599. Go to original source... Go to PubMed...
    27. Tann H., Soytong K. (2017): Bioformulations and nanoproduct from Chaetomium cupreum CC3003 to control leaf spot of rice var. Sen Pidoa in Cambodia. International Journal of Plant Biology, 7: 59-63. Go to original source...
    28. Tann Y., Soytong K. (2016): Effects of nanoparticles loaded with Chaetomium globosum KMITL0805 extracts against leaf spot of rice var Sen Pidoa. Malaysian Applied Biology, 45: 1-7. Go to original source...
    29. Thohinung S., Kanokmedhakul S., Kanokmedhakul K., Kukongviriyapan V., Tusskorn O., Soytong K. (2010): Cytotoxic 10-(indol-3-yl)-[13]cytochalasans from the fungus Chaetomium elatum ChE01. Archives of Pharmacal Research. 33: 1135-1141. Go to original source... Go to PubMed...
    30. Uehara K. (1958): On the production of phytoalexin by the host plant as a result of interaction between the rice plant and the blast fungus (Pyricularia oryzae Cav.). Annals of the Phytopathological Society of Japan, 23: 127-130. Go to original source...
    31. Vilavong S., Soytong K. (2017): Application of a new bioformulation of Chaetomium cupreum for biocontrol of Colletotrichum gloeosporioides causing coffee anthracnose on arabica variety in Laos. AGRIVITA, Journal of Agricultural Science, 39: 303-310. Go to original source...
    32. Yew S.M., Chan C.L., Lee K.W., Na S.L., Tan R., Hoh C.C., Yee W.Y., Ngeow Y.F., Ng K.P. (2014): A five-year survey of dematiaceous fungi in a tropical hospital reveals potential opportunistic species. PLoS ONE 9(8): e104352. doi. org/10.1371/journal.pone.0104352 Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content