Plant Protect. Sci., 2021, 57(4):263-270 | DOI: 10.17221/191/2020-PPS

Molecular characterisation of Zucchini yellow mosaic virus infecting Cucurbita pepo in EgyptOriginal Paper

Engy E. Abdel Aleem*, Mona Rabie, Faiza A. Fattouh
Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt

The complete nucleotide sequence of Zucchini yellow mosaic virus isolate from Egypt (ZYMV-Egz_MT383108) was determined. The sequence comparisons suggested that the isolate belongs to Group A. The sequence analysis of the Egyptian isolate showed the highest similarity (~96-97%) with the isolates leaf1 (KJ923767.1) and PA_2006 (JQ716413.1) from the USA and the lowest similarity (84%) with an isolate (AF014811.2) from Singapore. The phylogenetic analysis revealed that ZYMV-Egz occupied a distinct clade together with the USA isolates in Group A, known to be the most widespread throughout the world. This is a first record of the complete nucleotide sequence of an Egyptian isolate of ZYMV.

Keywords: cucurbits; genome sequence; phylogenetic analysis; RT-PCR; virus identification

Published: September 26, 2021  Show citation

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Abdel Aleem EE, Rabie M, Fattouh FA. Molecular characterisation of Zucchini yellow mosaic virus infecting Cucurbita pepo in Egypt. Plant Protect. Sci. 2021;57(4):263-270. doi: 10.17221/191/2020-PPS.
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