Plant Protect. Sci., 2021, 57(4):297-309 | DOI: 10.17221/185/2020-PPS

Toxicity and repellent activities of Thymus pallescens and Cymbopogon citratus essential oils against Sitophylus granariusOriginal Paper

Dahou Moutassem ORCID...1, Yuva Bellik1, Mohamed El Hadi Sannef2
1 Laboratory of Characterization and Valorization of Natural Resources, Faculty of Nature and Life Sciences, Mohamed El Bachir El Ibrahimi University, Bordj Bou Arreridj, Algeria
2 National High School of Computer Science, Algiers, Algeria

This study evaluated the toxicity and repellent activities of essential oils (EOs) against Sitophilus granarius (Linnaeus, 1758), both in vitro and in vivo conditions. The EOs obtained from Thymus pallescens Noë. (Lamiaceae) and Cymbopogon citratus Stapf. (Poaceae) were analyzed by GC-MS, and revealed that carvacrol (56.64%) and geraniol (20.8%) as the respective major components. T. pallescens EO was found to be a more effective toxicant, with LC50 and LC90 values of 9.3 and 34.6 µL/mL, respectively, in the contact test vs 8.2 and 25.3 µL/mL in the fumigation test. T. pallescens EO showed also a stronger repellent effect with values ranging from 83.4% to 100%. In the in vivo test, the survival probability decreased from 99.9 to 2.48% among insects exposed to T. pallescens EO. These data demonstrated that EOs could be considered effective alternatives to chemical insecticides, providing pest control for stored products in an ecologically sustainable manner.

Keywords: contact toxicity; corrected mortality; fumigation toxicity; metabolic profile; survival probability

Published: September 26, 2021  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Moutassem D, Bellik Y, Sannef MEH. Toxicity and repellent activities of Thymus pallescens and Cymbopogon citratus essential oils against Sitophylus granarius. Plant Protect. Sci. 2021;57(4):297-309. doi: 10.17221/185/2020-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abbott W.S. (1925): A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265-267. Go to original source...
    2. Abdelgaleil S.A.M., Abbassy M.A., Belal A.S.H., Abdel Rasoul M.A.A. (2008): Bioactivity of two major constituents isolated from the essential oil of Artemisia judaica L. Bioresource Technology, 99: 5947-5950. Go to original source... Go to PubMed...
    3. Abouelatta A.M., Keratum A.Y. Ahmed S.I., El-Zun H.M. (2020): Repellent, contact and fumigant activities of geranium (Pelargonium graveolens L.'hér) essential oils against Tribolium castaneum (Herbst) and Rhyzopertha dominica (F.). International Journal of Tropical Insect Science, 40: 1021-1030. Go to original source...
    4. Aulický R., Stejskal V. (2015): Efficacy and limitations of phosphine "spot-fumigation" against five Coleoptera species of stored product pests in wheat in a grain store - Short note. Plant Protection Science, 51: 33-38. Go to original source...
    5. Bassolé I.H.N., Lamien-Meda A., Bayala B., Obame L.C., Ilboudo A.J., Franz C., Novak J., Nebié R.C., Dicko M.H. (2011): Chemical composition and antimicrobial activity of Cymbopogon citratus and Cymbopogon giganteus essential oils alone and in combination. Phytomedicine, 18: 1070-1074. Go to original source... Go to PubMed...
    6. Bell C.H. (2011): Insect and mite penetration and contamination of packaged foods. In: Food and Beverage Stability and Shelf Life. Sawston, Woodhead Publishing Limited: 106-131. Go to original source...
    7. Benelli G., Flamini G., Canale A., Molfetta I., Cioni P.L., Conti B. (2012): Repellence of Hyptis suaveolens whole essential oil and major constituents against adults of the granary weevil Sitophilus granarius. Bulletin of Insectology, 65: 177-183.
    8. Benchabane O., Hazzit M., Mouhouche F., Baaliouamer A. (2015): Influence of extraction duration on the chemical composition and biological activities of essential Oil of Thymus pallescens de Noé. Arabian Journal for Science and Engineering, 40: 1855-1865. Go to original source...
    9. Bouzar Essaïdi K., Allal-Benfekih L., Djazouli Z.E. (2014): Comparative effects of biological treatments alone or in combination on the energy reserve contents of caterpillars of the pine processionary Thaumetopea pytiocampa schiff (Lepidoptera, Notodontidae). Revue Agrobiologia, 6: 47-52.
    10. Bradford M.M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254. Go to original source...
    11. Camaroti J.R.S.L., de Almeida W.A., do Rego Belmonte B., de Oliveira A.P.S., de Albuquerque Lima T., Ferreira M.R.A., Paiva P.M.G., Soares L.A.L., Pontual E.V., Napoleão T.H. (2018): Sitophilus zeamais adults have survival and nutrition affected by Schinus terebinthifolius leaf extract and its lectin (SteLL). Industrial Crops and Products, 116: 81-89. Go to original source...
    12. Campolo O., Giunti G., Russo A., Palmeri V., Zappalà L. (2018): Essential oils in stored product insect pest control. Journal of Food Quality, 2018: 6906105. doi: 10.1155/2018/6906105 Go to original source...
    13. Denholm I., Devine G. (2013): Insecticide Resistance. In: Levin S.A. (ed.): Encyclopedia of Biodiversity. 2nd ed. Cambridge, Academic Press: 298-307. Go to original source...
    14. Ditzen M., Pellegrino M., Vosshall L.B. (2008): Insect odorant receptors are molecular targets of the insect repellent DEET. Science, 319: 1838-1842. Go to original source... Go to PubMed...
    15. Essoung F.R.E., Tadjong A.T., Chhabra S.C., Mohamed S.A., Hassanali A. (2020): Repellence and fumigant toxicity of essential oils of Ocimum gratissimum and Ocimum kilimandscharicum on Tuta absoluta (Lepidoptera: Gelechiidae). Environmental Science and Pollution Research, 27: 37963-37976. Go to original source... Go to PubMed...
    16. Fornal J., Jeliñski T., Sadowska J., Grundas S., Nawrot J., Niewiada A., Warchalewski J.R., B³aszczak W. (2007): Detection of granary weevil Sitophilus granarius (L.) eggs and internal stages in wheat grain using soft X-ray and image analysis. Journal of Stored Products Research, 43: 142-148. Go to original source...
    17. Gaire S., Scharf M.E., Gondhalekar A.D. (2019): Toxicity and neurophysiological impacts of plant essential oil components on bed bugs (Cimicidae: Hemiptera). Scientific Reports, 9: 3961. doi: 10.1038/s41598-019-40275-5 Go to original source... Go to PubMed...
    18. Germinara G.S., De Cristofaro A., Rotundo G. (2015): Repellents effectively disrupt the olfactory orientation of Sitophilus granarius to wheat kernels. Journal of Pest Science, 88: 675-684. Go to original source...
    19. Gong X., Ren Y. (2020): Larvicidal and ovicidal activity of carvacrol, p-cymene, and γ-terpinene from Origanum vulgare essential oil against the cotton bollworm, Helicoverpa armigera (Hübner). Environmental Science and Pollution Research, 27: 18708-18716. Go to original source... Go to PubMed...
    20. Guettal S., Tine S., Hamaidia K., Tine-Djebbar F., Soltani N. (2021): Effect of Citrus limonum essential oil against granary weevil, Sitophilus granarius and its chemical composition, biological activities and energy reserves. International Journal of Tropical Insect Science, 41: 1531-1541 Go to original source...
    21. Hagstrum D.W., Subramanyam B. (2006): Fundamentals of Stored-Product Entomology. St. Paul, Woodhead Publishing and AACC International Press. Go to original source...
    22. Huang Y., Ho S.H., Lee H.C., Yap Y.L. (2002): Insecticidal properties of eugenol, isoeugenol and methyleugenol and their effects on nutrition of Sitophilus zeamais Motsch. (Coleoptera: curculionidae) and Tribolium castaneum (Herbst) (Coleoptera: tenebrionidae). Journal of Stored Products Research, 38: 403-412. Go to original source...
    23. Isman M.B. (2006): Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology, 51: 45-66. Go to original source... Go to PubMed...
    24. Isman M.B., Miresmailli S., MacHial C. (2011): Commercial opportunities for pesticides based on plant essential oils in agriculture, industry and consumer products. Phytochemistry Reviews, 10: 197-204. Go to original source...
    25. Keskin S., Ozkaya H. (2015): Effect of storage and insect infestation on the technological properties of wheat. CyTA - Journal of Food, 13: 134-139. Go to original source...
    26. Kim S.Il., Yoon J.S., Jung J.W., Hong K.B., Ahn Y.J., Kwon H.W. (2010): Toxicity and repellency of origanum essential oil and its components against Tribolium castaneum (Coleoptera: Tenebrionidae) adults. Journal of Asia-Pacific Entomology, 13: 369-373. Go to original source...
    27. Kordali S., Kotan R., Mavi A., Cakir A., Ala A, Yildirim A. (2005): Determination of the chemical composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the antifungal and antibacterial activities of Turkish Artemisia absinthium, Artemisia dracunculus, Artemisia santonicum and Artemisia spicigera essential oils. Journal of Agricultural and Food Chemistry, 53: 9452-9458. Go to original source... Go to PubMed...
    28. Kordali S., Yildirim E., Yazici G., Emsen B., Kabaagac G., Ercisli S. (2012): Fumigant toxicity of essential oils of nine plant species from asteraceae and clusiaceae against Sitophilus granarius (L.) (Coleoptera: Curculionidae). Egyptian Journal of Biological Pest control, 22: 11-14.
    29. Lee Y., Kim S.H., Montell C. (2010): Avoiding DEET through insect gustatory receptors. Neuron, 67: 555-561. Go to original source... Go to PubMed...
    30. Lengai G.M.W., Muthomi J.W., Mbega E.R. (2020): Phytochemical activity and role of botanical pesticides in pest management for sustainable agricultural crop production. Scientific African, 7: 1-3. Go to original source...
    31. Lu X., Weng H., Li C., He J., Zhang X., M.Z. (2020): Efficacy of essential oil from Mosla chinensis Maxim. cv. Jiangxiangru and its three main components against insect pests Industrial. Crops & Products, 147: 112-237. Go to original source...
    32. Magan N., Hope R., Cairns V., Aldred D. (2003): Post-harvest fungal ecology: Impact of fungal growth and mycotoxin accumulation in stored grain. European Journal of Plant Pathology, 109: 723-730. Go to original source...
    33. Marsin A.M., Muhamad I.I., Anis S.N.S., Lazim N.A.M., Ching L.W., Dolhaji N.H. (2020): Essential oils as insect repellent agents in food packaging: a review. European Food Research and Technology, 246: 1519-1532. Go to original source...
    34. Martínez L.C., Plata-Rueda A. Colares H.C., Campos J.M., Dos Santos M.H., Fernandes F.L., Serrão J.E., Zanuncio J.C. (2018): Toxic effects of two essential oils and their constituents on the mealworm beetle, Tenebrio molitor. Bulletin of Entomological Research, 108: 716-725. Go to original source... Go to PubMed...
    35. McDonald L.L., Guey R.H., Speir R.D. (1970): Preliminary Evaluation of New Candidate Materials as toxicants, Repellents and Attractants against Stored Product Insects. Marketing Research Report No. 882, Agricultural Research Service, U.S Department of Agriculture, Washington DC, USA.
    36. Morgan E.D. (2004): Biosynthesis in Insects. Cambridge, Royal Society of Chemistry. Go to original source...
    37. Moutassem D., Belabid L., Bellik Y., Ziouche S., Baali F. (2019): Efficacy of essential oils of various aromatic plants in the biocontrol of Fusarium wilt and inducing systemic resistance in chickpea seedlings. Plant Protection Science, 55: 202-217. Go to original source...
    38. Mutlu Ç., Öğreten A., Kaya C., Mamay M. (2019): Influence of different grain storage types on Khapra beetle, Trogoderma granarium Everts, 1898 (Coleoptera: Dermestidae), infestation in southeastern Anatolia (Turkey) and its resistance to malathion and deltamethrin. Turkish Journal of Entomology, 43: 131-142. Go to original source...
    39. Nawrot J., Gawlak M., Szafranek J., Szafranek B., Synak E., Warchalewski J.R., Piasecka-Kwiatkowska D., B³aszczak W., Jeliñski T., Fornal J. (2010): The effect of wheat grain composition, cuticular lipids and kernel surface microstructure on feeding, egg-laying, and the development of the granary weevil, Sitophilus granarius (L.). Journal of Stored Products Research, 46: 133-141. Go to original source...
    40. Oliveira A.P., Santos A.A., Santana A.S., Lima A.P.S., Melo C.R., Santana E.D.R., Sampaio T.S., Blank A.F., Araújo A.P.A., Cristaldo P.F., Bacci L. (2018): Essential oil of Lippia sidoides and its major compound thymol: Toxicity and walking response of populations of Sitophilus zeamais (Coleoptera: Curculionidae). Crop Protection, 112: 33-38. Go to original source...
    41. Papachristos D.P., Stamopoulos D.C. (2001): Repellent, toxic and reproduction inhibitory effects of essential oil vapours on Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae). Journal of Stored Products Research, 38: 117-128. Go to original source...
    42. Plaistow S.J., Bollache L., Cézilly F. (2003): Energetically costly precopulatory mate guarding in the amphipod Gammarus pulex: Causes and consequences. Animal Behaviour, 65: 683-691. Go to original source...
    43. Plata-Rueda A., Campos J.M, da Silva Rolim G., Martínez L.C, Dos Santos M.H., Fernandes F.L, Serrão J.E., Zanuncio JC. (2018): Terpenoid constituents of cinnamon and clove essential oils cause toxic effects and behavior repellency response on granary weevil, Sitophilus granarius. Ecotoxicology and Environmental Safety, 156: 263-270. Go to original source... Go to PubMed...
    44. Plata-Rueda A., Martínez LC., Rolim G.S., Coelho R.P., Santos M.H., Tavares W.S., Zanuncio J.C., Serrão J.E. (2020): Insecticidal and repellent activities of Cymbopogon citratus (Poaceae) essential oil and its terpenoids (citral and geranyl acetate) against Ulomoides dermestoides. Crop Protection, 137: 105299. doi: org/10.1016/j.cropro.2020.105299 Go to original source...
    45. Proctor D.L. (1994): Grain Storage Techniques: Evolution and Trends in Developing Countries. De Food and Agriculture Organization of the United Nations services Bulletin, 109.
    46. Ranjini K.D., Ranjini K.R., Beegum T.P.N., Nambiar J.G. (2016): Botanical extracts influence the protein and free amino acid concentration of fat body in the mango leaf webber Orthaga exvinacea Hampson (Lepidoptera: Pyralidae). Journal of Entomology and Zoology Studies, 4: 464-468.
    47. Rosentrater K.A, Evers A.D. (2018): Introduction to cereals and pseudocereals and their production. In: Kent's Technology of Cereals. 5th ed. Amsterdam, Elsevier Ltd. Go to original source...
    48. Senthil-Nathan S. (2013): Physiological and biochemical effect of neem and other Meliaceae plants secondary metabolites against Lepidopteran insects. Frontiers in Physiology, 4: 359. doi: 10.3389/fphys.2013.00359 Go to original source... Go to PubMed...
    49. Sönmez E., Gülel A. (2008): Effects of different temperatures on the total carbohydrate, lipid and protein amounts of the bean beetle, Acanthoscelides obtectus Say (Coleoptera: Bruchidae). Pakistan journal of Biological Sciences, 11: 1803-1808. Go to original source... Go to PubMed...
    50. Stephensons M.C. (1983): A study of population growth of Sitophilus oryzae L. and Sitophilus granarius L. in single and mixed culture in wheat and rice. [PhD. Thesis.] Palmerston North City, Massey University.
    51. Tapondjou A.L., Adler C., Fontem D.A., Bouda H., Reichmuth C. (2005): Bioactivities of cymol and essential oils of Cupressus sempervirens and Eucalyptus saligna against Sitophilus zeamais Motschulsky and Tribolium confusum du Val. Journal of Stored Products Research, 41: 91-102. Go to original source...
    52. Tarigan S.I., Dadang S., Harahap I. (2016): Toxicological and physiological effects of essential oils against Tribolium castaneum (Coleoptera: Tenebrionidae) and Callosobruchus maculatus (Coleoptera: Bruchidae). Journal of Biopesticides, 9: 135-147. Go to original source...
    53. Teke M.A., Mutlu Ç. (2020): Insecticidal and behavioral effects of some plant essential oils against Sitophilus granarius L. and Tribolium castaneum (Herbst). Journal of Plant Diseases and Protection, 128: 109-119. Go to original source...
    54. Tong F., Gross A.D., Dolan M.C., Coats J.R. (2013): The phenolic monoterpenoid carvacrol inhibits the binding of nicotine to the housefly nicotinic acetylcholine receptor. Pest Management Science, 69: 775-780. Go to original source... Go to PubMed...
    55. Trivedi A., Nayak N., Kumar J. (2018): Recent advances and review on use of botanicals from medicinal and aromatic plants in stored grain pest management. Journal of Entomology and Zoology Studies, 6: 295-300.
    56. Tuberoso C.I.G., Kowalczyk A., Sarritzu E., Cabras P. (2007): Determination of antioxidant compounds and antioxidant activity in commercial oil seeds for foods use. Food Chemistry, 103: 1494-1501. Go to original source...
    57. Tyler P.S., Binns T.J. (1982): The influence of temperature on the susceptibility to eight organophosphorus insecticides of susceptible and resistant strains of Tribolium castaneum, Oryzaephilus surinamensis and Sitophilus granarius. Journal of Stored Products Research, 18: 13-19. Go to original source...
    58. Van Handel E. (1985a): Rapid determination of glycogen and sugars in mosquitoes. Journal of the American Mosquito Control Association, 1: 299-301. Go to PubMed...
    59. Van Handel E. (1985b): Rapid determination of total lipids in mosquitoes. Journal of the American Mosquito Control Association, 1: 302-304. Go to PubMed...
    60. Vélez M., Barbosa W.F., Quintero J., Chediak M., Guedes R.N.C. (2017): Deltamethrin- and spinosad-mediated survival, activity and avoidance of the grain weevils Sitophilus granarius and S. zeamais. Journal of Stored Products Research, 74: 56-65. Go to original source...
    61. Vijayaraghavan C., Sivakumar C., Kavitha Z., Sivasubramanian P. (2010): Effect of plant extracts on biochemical components of cabbage leaf webber, Crocidolomia binotalis Zeller. Journal of Biopesticides, 3: 275-277.
    62. Yang Y., Isman M.B., Tak J.H. (2020): Insecticidal activity of 28 essential oils and a commercial product containing Cinnamomum cassia bark essential oil against Sitophilus zeamais Motschulsky. Insects, 11: 474. doi: 10.3390/insects11080474 Go to original source... Go to PubMed...
    63. Zettler J.L., Arthur F.H. (2000): Chemical control of stored product insects with fumigants and residual treatments. Crop Protection, 19: 577-582. Go to original source...
    64. Ziaee M., Moharramipour S., Francikowski J. (2014): The synergistic effects of Carum copticum essential oil on diatomaceous earth against Sitophilus granarius and Tribolium confusum. Journal of Asia-Pacific Entomology, 17: 817-822. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content