Plant Protect. Sci., 2021, 57(4):344-348 | DOI: 10.17221/56/2021-PPS

Characterization of an isolate of Lettuce big-vein associated virus (LBVaV) detected in naturally infected tomato (Solanum lycopersicum L.) in SlovakiaShort Communication

Jana Tomašechová1,2, Lukáš Predajňa2, Daniel Mihálik1, Michaela Mrkvová1, Pavel Cejnar3, Katarína Šoltys4, Sead Sabanadzovic5, Miroslav Glasa ORCID...*,1,2
1 Department of Biology and Biotechnologies, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovak Republic
2 Biomedical Research Center of the Slovak Academy of Sciences, Institute of Virology, Bratislava, Slovak Republic
3 Department of Computing and Control Engineering, Faculty of Chemical Engineering, University of Chemistry and Technology in Prague, Prague, Czech Republic
4 Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovak Republic
5 Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, USA

A tomato plant (Solanum lycopersicum Linnaeus, labelled KVE) displaying virus-like symptoms, tested negative for common tomato viruses, was subjected to high-throughput sequencing (HTS) on the Illumina MiSeq platform using ribosomal RNA-depleted total RNA as a template. The analysis has revealed the contigs mapping to Lettuce big-vein associated virus (LBVaV). The near complete LBVaV-KVE sequence of RNA1 and RNA2 revealed 95.0 and 94.9% identity with the reference sequence, the same length of translated products and a typical varicosavirus genome organisation. After initial long-term maintenance of LBVaV-KVE in the original plant, the virus could be detected by RT-PCR or nanoLC-ESI-Q-TOF in new plants generated from lateral shoot cuttings or inoculated by stem chips, although not uniformly. So far, LBVaV was reported to infect lettuce and related species. Our study expands the natural host range of the LBVaV to tomato.

Keywords: genome; next generation sequencing; Solanaceae; varicosavirus

Published: September 26, 2021  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Tomašechová J, Predajňa L, Mihálik D, Mrkvová M, Cejnar P, Šoltys K, et al.. Characterization of an isolate of Lettuce big-vein associated virus (LBVaV) detected in naturally infected tomato (Solanum lycopersicum L.) in Slovakia. Plant Protect. Sci. 2021;57(4):344-348. doi: 10.17221/56/2021-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bernal-Vicente A., Donaire L., Torre C., Gómez-Aix C., Sánchez-Pina M.A., Juarez M., Hernando Y., Aranda M.A. (2018): Small RNA-Seq to characterize viruses responsible of Lettuce Big Vein Disease in Spain. Frontiers in Microbiology, 9: 3188. doi: 10.3389/fmicb.2018.03188 Go to original source... Go to PubMed...
    2. Cejnar P., Kučková Š., Šantrůček J., Glasa M., Komínek P., Mihálik D., Slavíková L., Leišová-Svobodová L., Smirnova T., Hynek R., Kundu J.K., Ryšánek P. (2020): Efficient confirmation of plant viral proteins and identification of specific viral strains by nanoLC-ESI-Q-TOF using single-leaf-tissue samples. Pathogens, 9: 966. doi: 10.3390/pathogens9110966 Go to original source... Go to PubMed...
    3. Maccaron L.D. (2013): Relationships between the pathogen Olpidium virulentus and viruses associated with Lettuce Big-Vein Disease. Plant Disease, 97: 700-707. Go to original source... Go to PubMed...
    4. Maclot F., Candresse T., Filloux D., Malmstrom C.M., Roumagnac P., van der Vlugt R., Massart S. (2020): Illuminating an ecological blackbox: Using high throughput sequencing to characterize the plant virome across scales. Frontiers in Microbiology, 11: 578064. doi: 10.3389/fmicb.2020.578064 Go to original source... Go to PubMed...
    5. Maree H.J., Fox A., Al Rwahnih M., Boonham N., Candresse T. (2018): Application of HTS for routine plant virus diagnostics: State of the art and challenges. Frontiers in Plant Science, 9: 1082. doi: 10.3389/fpls.2018.01082 Go to original source... Go to PubMed...
    6. Sasaya T., Ishikawa K., Kuwata S., Koganezawa H. (2005): Molecular analysis of coat protein coding region of Tobacco stunt virus shows that it is a strain of Lettuce big-vein virus in the genus Varicosavirus. Archives of Virology, 150: 1013-1021. Go to original source... Go to PubMed...
    7. Sasaya T., Kusaba S., Ishikawa K., Koganezawa H. (2004): Nucleotide sequence of RNA2 of lettuce big-vein virus and evidence for a possible transcription termination/initiation strategy similar to that of rhabdoviruses. Journal of General Virology, 85: 2709-2717. Go to original source... Go to PubMed...
    8. Sasaya T., Ishikawa K., Koganezawa H. (2002): The nucleotide sequence of RNA1 of Lettuce big-vein virus, genus Varicosavirus, reveals its relation to nonsegmented negativestrand RNA viruses. Virology, 297: 289-297. Go to original source... Go to PubMed...
    9. Sasaya T., Fujii H., Ishikawa K., Koganezawa H. (2008): Further evidence of Mirafiori lettuce big-vein virus but not of Lettuce big-vein associated virus with big-vein disease in lettuce. Phytopathology, 98: 464-468. Go to original source... Go to PubMed...
    10. Tomašechová J., Hančinský R., Predajňa L., Kraic J., Mihálik D., Šoltys K., Vávrová S., Böhmer M., Sabanadzovic S., Glasa M. (2020): High-throughput sequencing reveals Bell pepper endornavirus infection in pepper (Capsicum annum) in Slovakia and enables its further molecular characterization. Plants, 9: 41. doi: 10.3390/plants9010041 Go to original source... Go to PubMed...
    11. Verbeek M., Dullemans A.M., van Bekkum P.J., van der Vlugt R.A.A. (2013): Evidence for Lettuce big-vein associated virus as the causal agent of a syndrome of necrotic rings and spots in lettuce. Plant Pathology, 62: 444-451. Go to original source...
    12. Walker P.J., Blasdell K.R., Calisher C.H., Dietzgen R.G., Kondo H., Kurath G., Longdon B., Stone D.M., Tesh R.B., Tordo N., Vasilakis N., Whitfield A.E. (2018): ICTV virus taxonomy profile: Rhabdoviridae. Journal of General Virology, 99: 447-448. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content