Plant Protect. Sci., 2023, 59(3):217-232 | DOI: 10.17221/64/2022-PPS

Compatibility of adzuki bean (Vigna angularis) and Bradyrhizobium USDA strains, and geographical distribution and community structure on indigenous adzuki bean-nodulating bradyrhizobia in JapanOriginal Paper

Sokichi Shiro1*, Ryu Makihara2, Masahiro Yamaguchi3, Masayuki Kadowaki1, Yuichi Saeki4
1 Institute of Agricultural and Life Sciences, Academic assembly, Shimane University, Shimane, Japan
2 Major in Agricultural and Life Sciences, Graduate School of Natural Science and Technology, Shimane University, Shimane, Japan
3 Department of Agricultural and Forest Sciences, Faculty of Life and Environmental Sciences, Shimane University, Shimane, Japan
4 Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan

We investigated the compatibility between adzuki bean and Bradyrhizobium USDA strains and the relation between the genetic diversity of indigenous adzuki bean-nodulating bradyrhizobia and their geographical distribution in Japan. Six Bradyrhizobium USDA strains were used in the inoculation test, and B. elkanii USDA 94 showed higher symbiotic efficiency than other strains. Two adzuki bean cultivars were used to isolate indigenous adzuki bean-nodulating bradyrhizobia. Their genetic diversity and community structure were analyzed by restriction fragment length polymorphisms of PCR amplicons to target the 16S-23S rRNA gene internal transcribed spacer region, using 11 USDA Bradyrhizobium strains as reference strains. Furthermore, we performed diversity analysis, non-metric multidimensional scaling analysis based on the Chao index, and polar ordination analysis to explain the relation between community structure and geographical distribution of the adzuki bean-nodulating bradyrhizobia. The major clusters were Bj123 in Hokkaido and Kyoto, Bd110 in Fukushima and Shimane A, Be76 in Shimane B and Fukuoka, Bj6 in Miyazaki, and Bj6 and BeOK in Okinawa. Japan's indigenous adzuki bean-nodulating bradyrhizobial community was strongly and significantly correlated with latitude, longitude, annual mean temperature, and electrical conductivity. This result suggests that the adzuki bean-nodulating bradyrhizobial community is influenced by multiple environmental factors.

Keywords: Bradyrhizobium; adzuki bean; compatibility; genetic diversity; environmental factors

Accepted: June 26, 2023; Prepublished online: September 5, 2023; Published: September 20, 2023  Show citation

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Shiro S, Makihara R, Yamaguchi M, Kadowaki M, Saeki Y. Compatibility of adzuki bean (Vigna angularis) and Bradyrhizobium USDA strains, and geographical distribution and community structure on indigenous adzuki bean-nodulating bradyrhizobia in Japan. Plant Protect. Sci. 2023;59(3):217-232. doi: 10.17221/64/2022-PPS.
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