Plant Protect. Sci., 2023, 59(4):325-336 | DOI: 10.17221/27/2023-PPS

A novel eggplant AP2/ERF transcription factor StPti5 confers resistance to Verticillium wilt across different speciesOriginal Paper

Nanyang Li1, Yinping Niu1, Xing Wang1, Suna Wang1, Yuhui Chen2, Liping Wang1
1 Laboratory of Plant Disease, Hebei University of Engineering, Han Dan, P. R. China
2 Laboratory of Eggplant Disease, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, P. R. China

The APETALA 2/ethylene response factors (AP2/ERFs) are considered essential in plant disease resistance responses. In this study, a novel eggplant AP2/ERF transcription factor gene, StPti5, was identified and functionally described from wild eggplant (Solanum torvum Sw.). The results of bioinformatics analysis show that StPti5 protein contains a DNA-binding AP2/ERF domain and shares high degree of amino acid similarity with the other known AP2/ERF domain. Subcellular localization assay shows that StPti5 protein was localized in the nucleus. Expression of StPti5 was induced by infection with Verticillium dahliae, ethylene, and methyl jasmonate. Analysis of expression patterns suggests that the function of StPti5 in resistance to Verticillium wilt was most closely linked to ethylene signaling. Overexpression of StPti5 in Arabidopsis thaliana could improve disease resistance to V. dahliae and activate genes linked to the ethylene signaling pathway. Studies on the A. thaliana defence response revealed that the formation of reactive oxygen species (ROS) increased considerably following overexpression of StPti5. In summary, our analysis suggest that StPti5 gene is a functional gene that could improve eggplant's resistance to V. dahliae.

Keywords: Solanum torvum; ERF transcription factors; Verticillium dahliae; disease resistance; ethylene signaling

Received: March 31, 2023; Revised: October 10, 2023; Accepted: October 18, 2023; Prepublished online: November 16, 2023; Published: November 28, 2023  Show citation

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Li N, Niu Y, Wang X, Wang S, Chen Y, Wang L. A novel eggplant AP2/ERF transcription factor StPti5 confers resistance to Verticillium wilt across different species. Plant Protect. Sci. 2023;59(4):325-336. doi: 10.17221/27/2023-PPS.
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    References

    1. Ali M., Ahmad H., Amin B., Atif M.J., Cheng Z. (2022): Induce defense response of DADS in eggplants during the biotrophic phase of Verticillium dahliae. BMC Plant Biology, 22: 1-15. Go to original source... Go to PubMed...
    2. Bletsos F., Thanassoulopoulos C., Roupakias D. (2003): Effect of grafting on growth, yield, and Verticillium wilt of eggplant. Hortscience, 38: 183-186. Go to original source...
    3. Cao Y., Wu Y., Zheng Z., Song F. (2006): Overexpression of the rice EREBP-like gene OsBIERF3 enhances disease resistance and salt tolerance in transgenic tobacco. Physiological and Molecular Plant Pathology, 67: 202-211. Go to original source...
    4. Chen K., Tang W., Zhou Y., Chen J., Xu Z., Ma R., Dong Y., Ma Y., et al. (2022): AP2/ERF transcription factor GmDREB1 confers drought tolerance in transgenic soybean by interacting with GmERFs. Plant Physiology and BioChemistry, 170: 287-295. Go to original source... Go to PubMed...
    5. Clough S.J., Bent A.F. (1998): Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant Journal, 16: 735-743. Go to original source... Go to PubMed...
    6. Dong L., Cheng Y., Wu J., Cheng Q., Li W., Fan S., Jiang L., Xu Z., et al. (2015): Overexpression of GmERF5, a new member of the soybean EAR motif-containing ERF transcription factor, enhances resistance to Phytophthora sojae in soybean. Journal of Experimental Botany, 66: 2635-2647. Go to original source... Go to PubMed...
    7. Dong N., Liu X., Lu Y., Du L.P., Xu H., Liu H., Xin Z., Zhang Z. (2010): Overexpression of TaPIEP1, a pathogen-induced ERF gene of wheat, confers host-enhanced resistance to fungal pathogen Bipolaris sorokiniana. Functional & Integrative Genomics, 10: 215-226. Go to original source... Go to PubMed...
    8. Eyal Y., Meller Y., Lev-Yadun S., Fluhr R. (1993): A basic-type PR-1 promoter directs ethylene responsiveness, vascular and abscission zone-specific expression. The Plant Journal, 4: 225-234. Go to original source... Go to PubMed...
    9. Fischer U., Dröge-Laser W. (2004): Overexpression of NtERF5, a new member of the tobacco ethylene response transcription factor family enhances resistance to Tobacco mosaic virus. Molecular Plant-Microbe Interactions, Go to original source...
    10. 17: 1162-1171.
    11. Fradin E.F., Thomma B.P.H.J. (2006): Physiology and molecular aspects of Verticillium wilt diseases caused by V. dahliae and V. albo-atrum. Molecular Plant Pathology, 7: 71-86. Go to original source... Go to PubMed...
    12. Fujimoto S.Y., Ohta M., Usui A., Shinshi, H., Ohme-Takagi M. (2000): Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression. The Plant Cell, Go to original source...
    13. 12: 393-404.
    14. Gao S., Zhang H., Tian Y., Li F., Zhang Z., Lu X., Chen X., Huang R. (2008): Expression of TERF1 in rice regulates expression of stress-responsive genes and enhances tolerance to drought and high-salinity. Plant Cell Reports, 27: 1787-1795. Go to original source... Go to PubMed...
    15. Gilmour S.J., Sebolt A.M., Salazar M.P., Everard J.D., Thomashow M.F. (2000): Overexpression of the Arabidopsis CBF3 transcriptional activator mimicsmultiple biochemical changes associated with cold acclimation. Plant Physiology, 124: 1854-1865. Go to original source... Go to PubMed...
    16. Glazebrook J., Chen W., Estes B., Chang H-S., Nawrath C., Métraux J-P., Zhu T., Katagiri F. (2003): Topology of the network integrating salicylate and jasmonate signal transduction derived from global expression phenotyping. The Plant Journal, 34: 217-228. Go to original source... Go to PubMed...
    17. Gu Y.Q., Yang C., Thara V.K., Zhou J., Martin G.B. (2000): Pti4 is induced by ethylene and salicylic acid, and its product is phosphorylated by the pto kinase. The Plant Cell, 12: 771-785. Go to original source...
    18. Guo W., Jin L., Miao Y., He X., Hu Q., Guo K., Zhu L., Zhang X. (2016): An ethylene response-related factor, GbERF1-like, from Gossypium barbadense improves resistance to Verticillium dahliae via activating lignin synthesis. Plant Molecular Biology, 91: 305-318. Go to original source... Go to PubMed...
    19. Guo Z.J., Chen X.J., Wu X.L., Ling J.Q., Xu P. (2004): Overexpression of the AP2/EREBP transcription factor OPBP1 enhances disease resistance and salt tolerance in tobacco. Plant Molecular Biology, 55: 607-618. Go to original source... Go to PubMed...
    20. Hawku M.D., Goher F., Islam M.A., Guo J., He F., Bai X., Yuan P., Kang Z., et al. (2021): TaAP2-15, An AP2/ERF transcription factor, is positively involved in wheat resistance to Puccinia striiformis f. sp. tritici. International Journal of Molecular Sciences, 22: 2080. doi:10.3390/ijms22042080 Go to original source... Go to PubMed...
    21. He P., Warren R.F., Shan L., Zhao T., Shan L., Zhu L., Tang X., Zhou J.M. (2001): Overexpression of Pti5 in tomato potentiates pathogen-induced defense gene expression and enhances disease resistance to pseudomonas syringae pv. tomato. Molecular Plant-Microbe Interactions, 14: 1453-1457. Go to original source... Go to PubMed...
    22. Li N.Y., Zhou L., Zhang D.D., Klosterman S.J., Li T.G., Gui Y.J., Kong Z.Q., Ma X.F., et al. (2018): Heterologous expression of the cotton NBS-LRR gene GbaNA1 enhances Verticillium Wilt resistance in Arabidopsis. Frontiers in Plant Science, 9: 119. doi:10.3389/fpls.2018.00119 Go to original source... Go to PubMed...
    23. Licausi F., Ohme-Takagi M., Perata P. (2013): APETALA2/Ethylene responsive factor (AP2/ERF) transcription factors: mediators of stress responses and developmental programs. New Phytologist, 199: 639-649. Go to original source... Go to PubMed...
    24. Liu J., Wang Y., Zhao G., Zhao J., Du H., He X., Zhang H. (2017): A novel Gossypium barbadense ERF transcription factor, GbERFb, regulation host response and resistance to Verticillium dahliae in tobacco. Physiology and Molecular Biology of Plants, 23: 125-134. Go to original source... Go to PubMed...
    25. Livak K.J., Schmittgen T.D. (2001): Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 25: 402-408. Go to original source... Go to PubMed...
    26. Mantiri F.R., Kurdyukov S., Lohar D.P., Sharopova N., Saeed N.A., Wang X.D., Vandenbosch K.A., Rose R.J. (2008): The transcription factor MtSERF1 of the ERF subfamily identified by transcriptional profiling is required for somatic embryogenesis induced by auxin plus cytokinin in Medicago truncatula. Plant Physiology, 146: 1622-1636. Go to original source... Go to PubMed...
    27. Mazarei M., Elling A.A., Maier T.R., Puthoff D.P., Baum T.J. (2007): GmEREBP1 is a transcription factor activating defense genes in soybean and Arabidopsis. Molecular Plant-Microbe Interactions, 20: 107-119. Go to original source... Go to PubMed...
    28. Meng X., Xu J., He Y., Yang K.Y., Mordorski B., Liu Y., Zhang S. (2013): Phosphorylation of an ERF transcription factor by Arabidopsis MPK3/MPK6 regulates plant defense gene induction and fungal resistance. The Plant Cell, 25: 1126-1142. Go to original source... Go to PubMed...
    29. Mishra S., Phukan U.J., Tripathi V., Singh D.K., Luqman S., Shukla R.K. (2015): PsAP2 an AP2/ERF family transcription factor from Papaver somniferum enhances abiotic and biotic stress tolerance in transgenic tobacco. Plant Molecular Biology, 89: 173-186. Go to original source... Go to PubMed...
    30. Mizoi, J., Shinozaki, K., Yamaguchi-Shinozaki K. (2012): AP2/ERF family transcription factors in plant abiotic stress responses. Biochimica et Biophysica Acta, 1819: 86-96. Go to original source... Go to PubMed...
    31. Moffat C.S., Ingle R.A., Wathugala D.L., Saunders N.J., Heather K., Knight H., Knight M.R. (2012): ERF5 and ERF6 play redundantroles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis. Plos One, 7: e35995. doi: 10.1371/journal.pone.0035995 Go to original source... Go to PubMed...
    32. Nakano T., Suzuki K., Fujimura T., Shinshi H. (2006): Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiology, 140: 411-432. Go to original source... Go to PubMed...
    33. Ohme-Takagi M., Shinshi H. (1995): Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. The Plant Cell, 7: 173-182. Go to original source... Go to PubMed...
    34. Oñate-Sanchez L., Anderson J.P., Young J., Singh K.B. (2007): AtERF14, a member of the ERF family of transcription factors, plays a nonredundant role in plant defense. Plant Physiology, 143: 400-409. Go to original source... Go to PubMed...
    35. Pré M., Atallah M., Champion A., De Vos M., Pieterse C.M., Memelink J. (2008): The AP2/ERF domain transcription factor ORA59 integrates jasmonic acid and ethylene signals in plant defense. Plant Physiology, 147: 1347-1357. Go to original source... Go to PubMed...
    36. Rashid M., He G., Yang G., Hussain J., Yan X. (2012): AP2/ERF transcription factor in Rice: genome-wide canvas and syntenic relationships between monocots and eudicots. Evolutionary Bioinformatics, 8: 321-355. Go to original source... Go to PubMed...
    37. Reymond P., Farmer E.E. (1998): Jasmonate and salicylate as global signals for defense gene expression. Current Opinion in Plant Biology, 5: 404-411. Go to original source... Go to PubMed...
    38. Santhanam P., Van Esse H.P., Albert I., Faino L., Nürnberger T., Thomma B.P.H.J. (2013): Evidence for functional diversification within a fungal NEP1-like protein family. Molecular Plant-microbe Interactions, 26: 278-286. Go to original source... Go to PubMed...
    39. Sharabi-Schwager M., Samach A., Porat R. (2009): Overexpression of the CBF2 transcriptional activator in Arabidopsis suppresses the responsiveness of leaf tissue to the stress hormone ethylene. Plant Biology, 12: 630-638. Go to original source... Go to PubMed...
    40. Shen L., Zhao E., Liu R., Yang X. (2022): Transcriptome analysis of eggplant under salt stress: AP2/ERF transcription factor SmERF1 acts as a positive regulator of salt stress. Plants, 11: 2205. doi:10.3390/plants11172205 Go to original source... Go to PubMed...
    41. Shoji T., Mishima M., Hashimoto T. (2013): Divergent DNA-Binding specificities of a group of ethylene response factor transcription factors involved in plant defense. Plant Physiology, 162: 977-990. Go to original source... Go to PubMed...
    42. Shu Y., Liu Y., Zhang J., Song L., Guo C. (2016): Genome-wide analysis of the AP2/ERF superfamily genes and their responses to abiotic stress in Medicago truncatula. Frontiers in Plant Science, 6: 1247. doi: 10.3389/fpls.2015.01247 Go to original source... Go to PubMed...
    43. Son G.H., Wan J., Kim H.J., Nguyen X.C., Chung W.S., Hong J.C., Stacey G. (2012): Ethylene-responsive element-binding factor 5, ERF5, is involved in chitin-induced innate immunity response. Molecular Plant-Microbe Interactions, 25: 48-60. Go to original source... Go to PubMed...
    44. Wally O., Punja Z.K. (2010): Genetic engineering for increasing fungal and bacterial disease resistance in crop plants. GM Crops, 14: 199-206. Go to original source... Go to PubMed...
    45. Wang N., Fan X., He M., Hu Z., Tang C., Zhang S., Lin D., Gan P., et al. (2022): Transcriptional repression of TaNOX10 by TaWRKY19 compromises ROS generation and enhances wheat susceptibility to stripe rust. The Plant Cell, 34: 1784-1803. Go to original source... Go to PubMed...
    46. Wang Y., Cui X., Bo Yang., Xu S., Wei X., Zhao P., Niu F., Sun M., et al. (2020): WRKY55 transcription factor positively regulates leaf senescence and the defense response by modulating the transcription of genes implicated in the biosynthesis of reactive oxygen species and salicylic acid in Arabidopsis. Development, 147: dev189647. doi:10.1242/dev.189647 Go to original source... Go to PubMed...
    47. Wu C., Avila C.A., Goggin F.L. (2015): The ethylene response factor Pti5 contributes to potato aphid resistance in tomato independent of ethylene signaling. Journal of Experimental Botany, 66: 559-570. Go to original source... Go to PubMed...
    48. Yamaguchi H., Fukuoka H., Arao T., Ohyama A., Nunome T., Miyatake K., Negoro S. (2010): Gene expression analysis in cadmium-stressed roots of a low cadmium-accumulating solanaceous plant, Solanum torvum. Journal of Experimental Botany, 61: 423-437. Go to original source... Go to PubMed...
    49. Yang L., Xie C., Li W., Zhang R., Jue D., Yang Q. (2013): Expression of a wild eggplant ribosomal protein L13a in potato enhances resistance to Verticillium dahliae. Plant Cell Tissue & Organ Culture, 115: 329-340. Go to original source...
    50. Yang X., Zhang Y., Cheng Y., Chen X. (2019): Transcriptome analysis reveals multiple signal network contributing to the Verticillium wilt resistance in eggplant. Scientia Horticulturae, 256: 108576. doi:10.1016/j.scienta.2019.108576 Go to original source...
    51. Yao Y., He R.J., Xie Q.L., Zhao X.H., Deng X.M., He J.B., Song L., He J., et al. (2017): Ethylene response factor 74 (ERF74) plays an essential role in controlling a respiratory burst oxidase homolog D (RbohD)-dependent mechanism in response to different stresses in Arabidopsis. New Phytologist, 213: 1667-1682. Go to original source... Go to PubMed...
    52. Zhao Y., Wei T., Yin K.Q., Chen Z., Gu H., Qu L.J., Qin G. (2012): Arabidopsis RAP2.2 plays an important role in plant resistance to Botrytis cinerea and ethylene responses. New Phytologist, 195: 450-460. Go to original source... Go to PubMed...
    53. Zhu W.,. Liu X., Chen M., TaoN., Tendu A., Yang Q. (2021): A New MiRNA MiRm0002 in eggplant participates in the regulation of defense responses to Verticillium Wilt. Plants, 10: 2274. doi: 10.3390/plants10112274 Go to original source... Go to PubMed...
    54. Zuo K.J., Qin J., Zhao J.Y., Ling H., Zhang L.D., Cao Y.F., Tang K.X. (2007): Over-expression GbERF2 transcription factor in tobacco enhances brown spots disease resistance by activating expression of downstream genes. Gene, 391: 80-90. Go to original source... Go to PubMed...

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