Plant Protect. Sci., 2024, 60(2):151-160 | DOI: 10.17221/106/2023-PPS

Preliminary study on horizontal transfer and loss of the AM toxin gene of AlternariaOriginal Paper

Ting Chen1#, Jiling Dang2,3#, Peng Zhang2, Jinju Shi2, Jia Feng3
1 Wuwei Agricultural Science Research Institute, Wuwei, Gansu, P. R. China
2 Shuifa Haohai (Jiuquan) Agricultural Development Co., Ltd, Jiuquan, Gansu, P. R. China
3 School of Life Science, Shanxi University, Taiyuan, Shanxi, P. R. China

The genus Alternaria has a global distribution and consists of a diverse group of pathogens. Plant-pathogenic Alternaria spp. can reduce the crop yield and pose serious threats to agricultural production. The pathogen A. mali is recognised as the key the pathogenic mechanism in the early defoliation of apples, which produces the host specific toxin (HST) that was named as an apple specific toxin (a specialised toxin of A. alternata pv. mali, AM toxin). The phenomenon of horizontal transfer of the AM toxin gene from different strains of A. alternata was found, and the relationship between the AM toxin and pathogenicity was confirmed. The representative strain A. tuberculata with the AM toxin gene was co-cultured with sixteen Alternaria strains without the AM toxin gene. As a result, four strains from different Alternaria species obtained the AM toxin gene, which indicated that the AM toxin gene can transfer among different Alternaria species. The AM toxin gene is easy to be lost after subculture, and high temperature and low nutrition can promote this loss. The symptoms of the Alternaria pathogen with or without the AM toxin gene are obviously different on the host. When infected by a pathogen with the AM toxin gene, green spots formed on the apple leaves, and rotten disease spots appeared in the fruit carpels. Contrary to this, when infected by a pathogen without this gene, only small epidermal spots without chlorosis formed on the apple leaves, and mildew-heart spots appeared in the fruit carpels.

Keywords: Alternaria spp.; AM toxin; horizontal transfer; pathogenicity

Received: October 24, 2023; Revised: March 21, 2024; Accepted: March 25, 2024; Prepublished online: April 23, 2024; Published: May 20, 2024  Show citation

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Chen T, Dang J, Zhang P, Shi J, Feng J. Preliminary study on horizontal transfer and loss of the AM toxin gene of Alternaria. Plant Protect. Sci. 2024;60(2):151-160. doi: 10.17221/106/2023-PPS.
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    References

    1. Akamatsu H., Taga M., Kodama M., Johnson R., Otani H., Kohmoto K. (1999): Molecular karyotypes for Alternaria plant pathogens known to produce host-specific toxins. Current Genetics, 35: 647-656. Go to original source... Go to PubMed...
    2. Andersen B., Hansen M.E., Smedsgaard J. (2005): Automated and unbiased image analyses as tools in phenotypic classification of small-spored Alternaria spp.. Phytopathology, 95: 1021-1029. Go to original source... Go to PubMed...
    3. Covert S.F. (1998): Supernumerary chromosomes in filamentous fungi. Current Genetics, 33: 311-319. Go to original source... Go to PubMed...
    4. Dang J.L. (2018): Phylogenetic analysis of Alternaria small-spored species and classification of Alternaria spp. on apple. Northwest A&F University, Yangling, Shaanxi, China.
    5. Friesen T.L., Stukenbrock E.H., Liu Z., Meinhardt S., Ling H., Faris J.D., Rasmussen J.B., Solomon P.S., et al. (2006): Emergence of a new disease as a result of interspecific virulence gene transfer. Nature Genetics, 38: 953-956. Go to original source... Go to PubMed...
    6. Hatta R.K., Itoh Y., Hosaki Y., Tanaka T., Tanaka A., Yamamoto M., Akimitsu K., Tsuge T. (2002): A conditionally dispensable chromosome controls host-specific pathogenicity in the fungal plant pathogen Alternaria alternata. Genetics, 161: 59-70. Go to original source... Go to PubMed...
    7. Jain R.M., Rivera C., Lake J.A. (1999): Horizontal gene transfer among genomes: the complexity hypothesis. Proceedings of the National Academy of Sciences of the United States of America, 96: 3801-3806. Go to original source... Go to PubMed...
    8. Johnson R.D, Johnson L., Itoh Y., Kodama M., Otani H., Kohmoto K. (2000): Cloning and characterization of a cyclic peptide synthetase gene from Alternaria alternata apple pathotype whose product is involved in AM-toxin synthesis and pathogenicity. Molecular Plant-Microbe Interactions, 13: 742-753. Go to original source... Go to PubMed...
    9. Johnson L.J., Johnson R.D., Akamatsu H., Salamiah A., Otani H., Kohmoto K., Kodama M. (2001): Spontaneous loss of a conditionally dispensable chromosome from Alternaria alternata apple pathotype leads to loss of toxin production and pathogenicity. Current Genetics, 40: 65-72. Go to original source... Go to PubMed...
    10. Kang Z.T., Jiang L.M., Luo Y.Y., Liu C.J., Li X.R. (2013): The research advances of mechanism of pathogenicity of alternaria phytopathogenic fungi. Chinese Bulletin of Life Sciences, 25: 908-914.
    11. Kistler H.C, Meinhardt L.W, Benny U. (1996): Mutants of Nectria haematococca created by a site-directed chromosome breakage are greatly reduced in virulence toward pea. Molecular Plant-Microbe Interactions, 9: 804-809. Go to original source...
    12. Liu X.M., Li D.Z. (2000): Researching advance of tobacco brown spot caused by Alternaria alternata. Journal of Northeast Agricultural University, 31: 81-85.
    13. Markham J.E, Hille J. (2001): Host-selective toxins as agents of cell death in plant-fungus interactions. Molecular Plant Pathology, 2: 229-239. Go to original source... Go to PubMed...
    14. Pryor B.M., Gilbertson R.L. (2000): Molecular phylogenetic relationships amongst Alternaria species and related fungi based upon analysis of nuclear ITS and mt SSU rDNA sequences. Mycology research, 104 : 1312-1321. Go to original source...
    15. Saha D., Fetzner R., Burkhardt B., Podlech J., Metzler M., Dang H., Lawrence C., Fischer R. (2012): Identification of a polyketide synthase required for alternariol (AOH) and alternariol-9-methyl ether (AME) formation in Alternaria alternata. Plos One, 7: 40-64. Go to original source... Go to PubMed...
    16. Sagarika D., Perumal K. (2014): Influence of culture condition and pH on growth and production of brown pigment from Alternaria alternata. International Journal of Scientific Research, 3: 458-461.
    17. VanEtten H., Jorgensen S., Enkerli J., Covert S.F. (1998): Inducing the loss of conditionally dispensable chromosomes in Nectria haematococca during vegetative growth. Current Genetics, 33: 299-303. Go to original source... Go to PubMed...
    18. Zhang C.X., Chen Y., Li Z., Zhang L.Y., Kang G.D., Cong P.H. (2012): Ultrastructure study of the interaction process between Alternaria mali and host leaves. Acta Botanica Boreali-Occidentalia Sinica, 1: 106-110.

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