Plant Protect. Sci., 2024, 60(2):161-171 | DOI: 10.17221/109/2023-PPS

The disease quantification analysis of cotton Verticillium wilt using the two methods of disease index and fungal biomass present high consistencyOriginal Paper

He Zhu1, Yue Li1, Xiao-Bin Ji2, Dan-Dan Zhang2,3, Jie-Yin Chen2,3, Xiao-Feng Dai2,3, Zi-Sheng Wang1, Dan Wang ORCID...2,4
1 National Cotton Industry Technology System Liaohe Comprehensive Experimental Station, The Cotton Research Center of Liaoning Academy of Agricultural Sciences, Liaoning Provincial Institute of Economic Crops, Liaoyang, P. R. China
2 The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
3 Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji, P. R. China
4 State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou, P. R. China

Verticillium dahliae is a broad host-range pathogen that causes vascular wilt in plants. The Verticillium wilt disease severity assay on plants caused by V. dahliae mainly includes two methods, one is a plant disease grade classification based on disease severity statistics [namely the disease index (DI)], and the other is the V. dahliae biomass quantification in plants (namely the fungal biomass). In this study, the relationships of pathogenicity with the DI, pathogenicity with the biomass, and the correlation analysis of the DI and relative fungal biomass were analysed. The results showed that pathogenicity assessment of V. dahliae strains using the DI method was able to give an intuitive reflection of the pathogenic ability for defoliating and non-defoliating strains; moreover, the method of quantitative PCR for fungal biomass also had high repeatability and stability. As a whole, the correlation coefficient between the DI and fungal biomass values of 28 strains was 0.728, indicating that the two data sets were highly correlated; however, the correlation coefficients of the defoliating strains and non-defoliating strains were only 0.5384 and 0.4547, respectively. In conclusion, the correlation coefficient between the DI and the fungal biomass presented high consistency, which could provide some meaningful exploration for the more accurate pathogenicity identification of V. dahliae.

Keywords: Verticillium dahliae; pathogenicity; disease grade classification; qPCR; correlation coefficient

Received: October 28, 2023; Revised: January 2, 2024; Accepted: January 15, 2024; Prepublished online: March 5, 2024; Published: May 20, 2024  Show citation

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Zhu H, Li Y, Ji X, Zhang D, Chen J, Dai X, et al.. The disease quantification analysis of cotton Verticillium wilt using the two methods of disease index and fungal biomass present high consistency. Plant Protect. Sci. 2024;60(2):161-171. doi: 10.17221/109/2023-PPS.
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