Plant Protect. Sci., 2024, 60(4):380-392 | DOI: 10.17221/54/2024-PPS

Transcriptomic analysis of melon with different Phelipanche aegyptiaca resistanceOriginal Paper

Yao Guo1,2, Juntao Yang2, Haojie Wang2, Junhua Li2, Bin Liu2, Haozhe Min2,3, Yongbing Zhang2*, Jiancai Mao2
1 Department of Horticulture, Faculty of Agriculture, Shihezi University, Shihezi, P. R. China
2 Hami-Melon Research Center, Xinjiang Academy of Agricultural Sciences, Urumqi, P. R. China
3 Department of Horticulture, College of Horticulture and Forestry, Talimu University, Alaer, P. R. China

To elucidate the genetic factors contributing to melon resistance against Phelipanche aegyptiaca and comprehend the role of differentially resistant materials in responding to changes in P. aegyptiaca parasitisation, we investigated the P. aegyptiaca-resistant line K16 and the susceptible line K27. The parasitism rate of P. aegyptiaca was assessed at 25 days. Results revealed significant differences in parasitisation rates between K16 (15.35%) and K27 (34.2%). We compared inoculated K16 and K27 to their respective controls through transcriptome analysis and contrasted inoculated K16 with inoculated K27. Eight hundred eighteen genes exhibited differential expression across all comparisons. Gene ontology (GO) functional enrichment analysis revealed that differentially expressed genes were significantly enriched in nitrate transport and assimilation, cellular components, extracellular regions, binding and enzyme activities. KEGG pathway enrichment underscored the importance of phytohormone signaling, phenylpropanoid biosynthesis, linolenic acid and linoleic acid metabolism, cyanoamino acid metabolism and nitrogen metabolism in the interaction between melon and P. aegyptiaca. Nine genes potentially associated with P. aegyptiaca resistance were identified, encoding cytochrome protein P450, peroxidases, β-glucosidase, acyltransferase family proteins, histidine phosphotransfer protein, and D-type cyclins. This study aims to provide insights into the mechanism of P. aegyptiaca parasitism on melons and offers implications for breeding resistant varieties

Keywords: melon; Phelipanche aegyptiaca (Pers.); transcriptome; differentially expressed genes

Received: April 9, 2024; Revised: July 4, 2024; Accepted: July 24, 2024; Prepublished online: September 6, 2024; Published: October 14, 2024  Show citation

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Guo Y, Yang J, Wang H, Li J, Liu B, Min H, et al.. Transcriptomic analysis of melon with different Phelipanche aegyptiaca resistance. Plant Protect. Sci. 2024;60(4):380-392. doi: 10.17221/54/2024-PPS.
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