Plant Protection Science, 1999 (vol. 35), issue 1

Molecular characterization and phylogeny of the entomopathogenic fungusOriginal Paper

Miroslav Oborník, Richard Stouthamer, Ellis Meekes, Menno Schilthuittzen

Plant Protect. Sci., 1999, 35(1):1-9 | DOI: 10.17221/9664-PPS  

We characterized 23 isolates of the entomopathogenic fungus Aschersonia spp. from Mexico, Brazil, Guyana, Trinidad, Venezuela, Columbia, Florida, Malaysia, Thailand, Japan, Philippines, Java and South India using RAPD markers. The data were used to compute the genetic variability and to reconstruct the phylogeny of the genus Aschersonia. Relative genetic distances varied from 0.018 (between isolates Aa2 and Ap2) to 0.445 (between isolates A1 and At1). In the constructed phylogenetic tree, isolates were clustered according to their geographical origin. We determined partial 26S ribosomal DNA sequences of five Aschersonia isolates (A28, A31,...

Chemical communication in horse-chestnut leafminer Cameraria ohridella Deschka & Dimić.Original Paper

Aleš Svatoš, Blanka Kalinová, Michal Hoskovec, Jiří Kindl, Ivan Hrdý

Plant Protect. Sci., 1999, 35(1):10-13 | DOI: 10.17221/9665-PPS  

Females of the horse-chestnut leafminer Cameraria ohridella Deschka & Dimić (Lepidoptera: Gracillariidae) produce a highly attractive sex pheromone in the early photophase. The pheromone, luring conspecific males into Delta traps baited with females, is produced in the female abdominal tip (ca 10-50 pg per calling female) and the active components can be extracted with hexane. Both dissected tips and their hexane extracts show high attractiveness for C. ohridella males in a wind tunnel behavioural bioassay. According to electrophysiologic recordings the male antennae are notably sensitive to both dissected female abdomens and extracts from them....

Development of Cheyletus eruditus (Schrank) (Acarina: Cheyletidae) at low temperatureOriginal Paper

Eva Ždarková, Pavel Horák

Plant Protect. Sci., 1999, 35(1):14-16 | DOI: 10.17221/9666-PPS  

Development of the predatory mite C. eruditus was examined at 12, 14 and 25°C and 75% r.h. 12°C is the low temperature threshold for development of this species where development lasts 164 days, compared with 47 days of its prey Acarus siro under the same conditions. Since repressive biological control cannot be successful under those circumstances, the predators can only be used preventively in empty stores.

Occurrence and bionomics of Eriborus terebrans (Gravenhorst) (Hymenoptera: Ichneumonidae), a parasitoid of the European corn borer, Ostrinia nubilalis Hbn. (Lepidoptera: Pyralidae), in Central EuropeOriginal Paper

Peter Bokor, Ľudovít Cagáň

Plant Protect. Sci., 1999, 35(1):17-22 | DOI: 10.17221/9667-PPS  

Eriborus terebrans (Gravenhorst), the parasitoid of the European corn borer, Ostrinia nubilalis Hbn., was studied at four locations in central Europe during 1993-1995. Regular parasitism of O. nubilalis was found only at Blatnice in Moravia (eastern part of Czech Republic). At this location, the parasitism was 2.22% in 1993, 0.47% in 1994 and 0.06% in 1995. In 1994 and 1995, low parasitism (0.56 and 0.12% ,respectively) was found at Kráľovský Chlmec (eastern Slovakia). The records are the first from Czech Republic and Slovakia. The parasitoid was not found at Nitra (south-western Slovakia) and Wroclaw (south-western Poland)....

Survival over the winter of Rhyzopertha dominica F. (Coleoptera: Bostrichidae) in hot spots caused by improper grain storage technology: the first record in the Czech RepublicOriginal Paper

Václav Stejskal, Jan Zuska, Petr Werner, Zuzana Kučerová

Plant Protect. Sci., 1999, 35(1):23-25 | DOI: 10.17221/9668-PPS  

The survival of dense populations of Rhyzopertha dominica in stored grain hot spots in the Czech Republic during winter months was documented for the first time. Improper storage of grain causes subsurface hot zones, which seem to provide conditions for pest overwintering and become the source of subsequent cross infestations.

Variability of resistance to common bunt of wheatOriginal Paper

Antonín Pospisil, Josef Benada, Ivana Polisenská

Plant Protect. Sci., 1999, 35(1):26-29 | DOI: 10.17221/9670-PPS

During 1997-1998, the reaction of the collections of winter and spring wheat varieties to Tilletia caries was determined. In both years the most diseased winter varieties were Simona and Sparta, the least diseased were Samara and Ilona. High variability in the level of infection was observed in some varieties (VIada, Estica, Regina, Vega). No winter wheat variety was completely resistant. The most diseased spring wheat variety was Alexandria in both years, while Grandur showed no infection. High variability in the level of infection was observed in the special trials with cv. Ina and Contra. In trials of the efficacy of seed...

Study of semipersistent transmission of beet yellows virus by the green peach aphid, Myzus persicae Sulz.Short Communication

Jiban Kumar Kundu, Pavel Ryšánek

Plant Protect. Sci., 1999, 35(1):30-32 | DOI: 10.17221/9669-PPS  

The optimal conditions for beet yellows virus (BYV) acquisition and transmission by Myzus persicae Sulz. were studied. Maximum recorded retention of BYV in aphids was 48 h. Preacquisition starvation of aphids influenced positively transmission efficiency if the acquisition access feeding period was short (30 min to 2 h), but after a longer such period it was without influence. The efficiency of transmission also depended on the acquisition access feeding period. Maximum transmission efficiency of starved and nonstarved aphids occurred after 2 h (75%) and 4-6 h (65%) of acquisition, respectively.

Virus diseases of poppy (Papaver somniferum L.) and some other species of the Papaveraceae family - a reviewReview

Darina Kubelková, Josef Špak

Plant Protect. Sci., 1999, 35(1):33-36 | DOI: 10.17221/9671-PPS  

Opium poppy (Papaver somniferum L.) is described in the literature as a natural host of turnip mosaic virus, bean yellow mosaic virus, beet yellows virus and beet mosaic virus, and experimental host of plum pox virus. P. orientale L., a natural host of beet curly top virus, was successfully infected with turnip mosaic virus and cucumber mosaic virus, and P. dubium L. with turnip mosaic virus. P. rhoeas L. is a natural host of turnip mosaic virus, and artificial host of beet yellows, plum pox and cucumber mosaic viruses. P. nudicaule is reported as a natural host of beet curly top, tomato spotted wilt viruses...