Plant Protect. Sci., 2003, 39(3):79-87 | DOI: 10.17221/3861-PPS

Induction of post-infection ethylene and its role in resistance of bean (Phaseolus vulgaris) against Colletotrichum lindemuthianum

James Ombiri, Volker Zinkernagel, Eliud M. Gathuru, Oliver Achwanya, Aleš Lebeda
1 Department of Botany, Egerton University, Njoro, Kenya; 2Plant Pathology, Department of
2 Plant Sciences, Center of Life and Food Sciences, TU Munich, Freising-Weihenstephan, Germany
3 Department of Botany, Faculty of Science, Palacký University, Olomouc, Czech Republic

The influence of inoculation techniques on induction of post-infection ethylene production in susceptible and resistant genotypes of bean (Phaseolus vulgaris) inoculated with the kappa race of Colletotrichum lindemuthianum was studied. Three inoculation methods (brushing, dipping and spraying) of excised leaves were compared. The brushing technique caused both the highest ethylene production at 120 h after inoculation, and better symptom development than dipping and spraying. It was, therefore, adopted to determine the post-infection ethylene production in four inoculated bean genotypes (GLP406 and Kaboon - resistant, GLP636 - moderately resistant; and MDRK - susceptible). Ethylene production increased slightly 24 h after inoculation in all four genotypes, followed by a rapid decline after 48 h. Ethylene production remained low until 120 h after which a sharp rise was observed in genotype MDRK. Increased production, though to a lesser extent, was observed in GLP636. This trend was repeatable and thus may offer an additional physiological marker to bean breeders to screen for resistance to C. lindemuthianum.

Keywords: anthracnose; bean; degree of resistance; inoculation techniques; physiological marker; race-specific resistance

Published: September 30, 2003  Show citation

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Ombiri J, Zinkernagel V, Gathuru EM, Achwanya O, Lebeda A. Induction of post-infection ethylene and its role in resistance of bean (Phaseolus vulgaris) against Colletotrichum lindemuthianum. Plant Protect. Sci. 2003;39(3):79-87. doi: 10.17221/3861-PPS.
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