Plant Protect. Sci., 2004, 40(4):121-127 | DOI: 10.17221/468-PPS

Biological and serological procedures to detect three nepoviruses in fruit trees

Jaroslav Polák, Michaela Chaloupková, Milan Jokeš
Department of Virology, Division of Plant Medicine, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic

Cherry leaf roll virus (CLRV), Myrobalan latent ringspot virus (MLRSV) and Strawberry latent ringspot virus (SLRSV) were transferred by budding to woody trees, hybrid Ishtara, peach cv. GF 305 and cv. Lesiberian. Three buffers with antioxidants and stabilisers: 0.01M phosphate with 1% caffeine; 0.007M phosphate-0.01M veronal with 0.01M cysteine hydrochloride and 0.007 EDTA; 0.015M phosphate with 1% nicotine and 0.066M phosphate buffer without additives were compared for their efficiency in mechanical transmission from woody sources to herbaceous hosts (Chenopodium quinoa and C. amaranticolor). 0.007M phosphate-0.01M veronal buffer with 0.01M cysteine hydrochloride, and 0.007 EDTA and 0.015M phosphate buffer with 1% nicotine were found to be the best buffers for the three nepoviruses. Both biological transmission to herbaceous assay hosts and detection by ELISA in the investigated tree are necessary to reliably detect the three nepoviruses. Biological detection is reliable from April to June, and in September and October. ELISA detection is also more difficult in July and August. The suitability of C. quinoa and C. amaranticolor to maintain CLRV, MLRSV and SLRSV was compared. C. amaranticolor plants were found to be more suitable for CLRV and SLRSV, infected plants grow over 6 months after mechanical inoculation by the nepoviruses. C. quinoa plants proved to be most suitable for maintenance of MLRSV, while C. amaranticolor is a symptomless host of MLRSV. Reinoculation with the nepoviruses is recommended in intervals of 4 to 6 months.

Keywords: Cherry leaf roll virus; Myrobalan latent ringspot virus; Strawberry latent ringspot virus; mechanical transmission; herbaceous hosts; bud transmission; fruit trees; DAS-ELISA; electron microscopy

Published: December 31, 2004  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Polák J, Chaloupková M, Jokeš M. Biological and serological procedures to detect three nepoviruses in fruit trees. Plant Protect. Sci. 2004;40(4):121-127. doi: 10.17221/468-PPS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. A L., C J. (1981): Identification of viruses in sweet cherry trees (Prunus avium L.) with symptoms similar to cherry leaf roll. In: Plant Virology. Proc. 9th Conf. Czechoslovak Plant Virologists, Brno: 17-20.
    2. B A.A., C K., D M.Y., G A.J., W L. (1996): Viruses of Plants. CAB International. Wallingford, Oxon, UK.
    3. D J., D R. (1976): Myrobalan latent ringspot, a bipartite genome virus and a strain of tomato black ring virus. Mi. Biol. Bundesanst. Land- Forstwirtsch. Berlin-Dahlem, 170: 9-16.
    4. G P., D P., C T., D J. (2001): Characterization of a new nepovirus infecting apricot in Southeastern France: apricot latent ringspot virus. Eur. J. Plant Pathol., 107: 485-494.
    5. J M., P Z. (1988): Výsledky testů na přítomnost virů v matečných rostlinách odrůd a novošlechtění maliníku. Zahradnictví, 14: 267-277.
    6. J A.T., M M.A. (1972): The two nucleoprotein particles of Cherry leaf roll virus. J. Gen. Virol., 16: 349-358. Go to original source...
    7. K J., P J. (1990): Diagnóza viru latentní kroužkovitosti jahodníku imunoenzymatickým testem (ELISA). Zahradnictví, 17: 299-303. Go to original source...
    8. L R.M. (1964): Strawberry latent ringspot: a new nematode-borne virus. Ann. Appl. Biol., 51: M 167-176. Natural spread, gra transmission, and possible etiology of walnut blackline disease. Phytopathology, 70: 962-968.
    9. N J.B., L J. (1975): K etiologii zhoubné rakoviny třešně. In: Sbor. Vysoké Školy Zemědělské, Praha, Řada A: 205-223.
    10. N J.B., L J. (1981): Virus svinutky třešně z ořešáku vlašského (Juglans regia) v Československu. Ochr. Rostl., 17: 1-8.
    11. T B.J. (1980): The detection by serological methods of viruses infecting the rose. Ann. Appl. Biol., 94: 91-101. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content