Plant Protect. Sci., 2019, 55(1):61-71 | DOI: 10.17221/20/2018-PPS

Selection and evaluation of potential reference genes for gene expression analysis in Avena fatuaOriginal Paper

Junjie Liu†, Peng Li†, Liuyang Lu, Lanfen Xie, Xiling Chen*, Baizhong Zhang*
College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, P.R. China

Eight commonly used candidate reference genes, 18S ribosomal RNA (rRNA) (18S), 28S rRNA (28S), actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1 alpha (EF1α), ribosomal protein L7 (RPL7), Alpha-tubulin (α-TUB), and TATA box binding protein-associated factor (TBP), were evaluated under various experimental conditions to assess their suitability in different developmental stages, tissues and herbicide treatments in Avena fatua. The results indicated the most suitable reference genes for the different experimental conditions. For developmental stages, 28S and EF1α were the optimal reference genes, both EF1α and 28S were suitable for experiments of different tissues, whereas for herbicide treatments, GAPDH and ACT were suitable for normalizations of expression data. In addition, GAPDH and EF1α were the suitable reference genes.

Keywords: wild oat; expression; herbicide

Published: March 31, 2019  Show citation

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Liu J, Li P, Lu L, Xie L, Chen X, Zhang B. Selection and evaluation of potential reference genes for gene expression analysis in Avena fatua. Plant Protect. Sci. 2019;55(1):61-71. doi: 10.17221/20/2018-PPS.
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