Plant Protection Science, 2019 (vol. 55), issue 1

List of Reviewers 2018

editors

Plant Protect. Sci., 2019, 55(1):I-II | DOI: 10.17221/11542-PPS  

Genetic diversity of the Cucumber green mottle mosaic virus and the development of RT-LAMP assay for its detectionShort Communication

Beata Hasiów-Jaroszewska, Daria Budzyńska, Natasza Borodynko-Filas

Plant Protect. Sci., 2019, 55(1):1-7 | DOI: 10.17221/92/2018-PPS  

To analyse the genetic diversity of the Cucumber green mottle mosaic virus (CGMMV) population in Poland and to establish the phylogenetic relationships between the Polish and other isolates described to date, 91 isolates were collected from cucumber plants. The analysis, based on coat protein (CP) gene, revealed the presence of two phylogenetic groups: one consisting of the Polish isolates collected in 2017 and those originated mainly from Asia region and the second including the Polish isolates collected in 2016 and the others from European countries. The sensitive, specific, and rapid one-step loop-mediated isothermal amplification...

Reaction of transgenic plum cv. HoneySweet to the Plum pox virus after a severe infection of Monilinia sp. - short communicationShort Communication

Jaroslav Polák, Tereza Neubauerová, Petr Komínek, Jiban Kumar Kundu

Plant Protect. Sci., 2019, 55(1):8-10 | DOI: 10.17221/152/2017-PPS  

Resistance to Plum pox virus (PPV) in transgenic Prunus domestica L., clone C5 (cv. HoneySweet) was evaluated in a regulated field in the Czech Republic for fifteen years (2002-2016). PPV mild symptoms appeared in C5 trees only in several leaves situated close to the point of inoculum grafting up to 2010. No symptoms of PPV were observed in the years 2011-2013 and results of ELISA and RT-PCR detection tests were negative. In the twelfth year (2013), there was a severe unusual natural attack of plum trees by Monilinia sp. This Monilinia sp. attack occurred only one time - in 2013. There was no Monilinia sp....

Categorisation of reactions and enumeration of bacteria in potato cultivars inoculated with the causal agent of bacterial ring rotOriginal Paper

Iveta Pánková, Václav Krejzar, Radka Krejzarová

Plant Protect. Sci., 2019, 55(1):11-22 | DOI: 10.17221/44/2018-PPS  

Variability in the responses of plants propagated from in vitro tissue cultures of 52 ware and industrial potato cultivars to different Clavibacter michiganensis subsp. sepedonicus inoculum size was tested during 2015-2017. Bacterial ring rot symptoms on plants and tubers xylem vessels were recorded for 8 weeks and the susceptibility index (SI) for individual cultivars was calculated. Based on foliage symptoms, potato cultivars were placed into three symptoms groups. The symptomless group had SIs ≤ 1, for the moderate symptom group SIs ranged from 1.01 to 2.99, and the severe symptom group had...

Impact of Piper betle leaf extract on grape downy mildew: effects of combining 4-allylpyrocatechol with eugenol, α-pinene or β-pineneOriginal Paper

Yoshinao Aoki, Nguyen Van Trung, Shunji Suzuki

Plant Protect. Sci., 2019, 55(1):23-30 | DOI: 10.17221/53/2018-PPS  

Methanol extract of Piper betle leaves exhibited an inhibitory effect on grape downy mildew. This extract might contain more than two compounds which have different polarities that suppress grape downy mildew. Gas chromatograph-tandem mass spectrometry analysis identified 4-allylpyrocatechol, eugenol, α-pinene, and β-pinene in the methanol extract. Neither of the compounds suppressed grape downy mildew by single treatment. On the other hand, treatment with a combination of 4-allylpyrocatechol with eugenol, α-pinene or β-pinene enhanced the inhibitory effects on grape downy mildew and perfectly suppressed it. The complex...

Effect of heat-shock pre-treatment on tomato plants infected by powdery mildew fungusOriginal Paper

Vladimíra Nožková, Barbora Mieslerová, Lenka Luhová, Jana Piterková, Ondřej Novák, Martina Špundová, Aleš Lebeda

Plant Protect. Sci., 2019, 55(1):31-42 | DOI: 10.17221/24/2018-PPS  

The effect of plant heat-shock (HS) pre-treatment (40.5°C, 2 h) on Pseudoidium neolycopersici development in the susceptible and moderately resistant Solanum spp. genotypes was studied together with biochemical responses (endogenous concentrations of salicylic (SA), jasmonic (JA), abscisic acid (ABA), and peroxidase (POX) activity). In HS pre-treated S. lycopersicum, an acceleration of pathogen, chlorosis and necrosis development, strong SA, JA accumulation, and increased POX activity were detected. In S. chmielewskii, HS pre-treatment caused a slight suppression of pathogen development, increase in JA, ABA concentrations,...

Potential source of environmentally benign antifungal agents from Cinnamomum osmophloeum leaves against Phellinus noxiusOriginal Paper

Sen-Sung Cheng, Chun-Ya Lin, Min-Jay Chung, Ying-Ju Chen, Shang-Tzen Chang

Plant Protect. Sci., 2019, 55(1):43-53 | DOI: 10.17221/28/2017-PPS  

The antifungal activity of leaf oils from different provenances of Cinnamomum osmophloeum was evaluated against Phellinus noxius and their chemical polymorphism. C. osmophloeum leaf oils of 15 provenances and their relative contents were classified into eight chemotypes, namely cinnamaldehyde, cinnamaldehyde/linalool, linalool, cinnamaldehyde/cinnamyl acetate, linalool/camphor, camphor/bornyl acetate, 1,8-cineole/p-cymene, and mixed types according to GC-MS, CA, and PCA. It was found that leaf oils of both cinnamaldehyde and cinnamaldehyde/cinnamyl acetate types had excellent inhibitory effects against P. noxius,...

Stable isotope labelling of Ceratitis capitataOriginal Paper

Hasan Al-Khshemawee, Manjree Agarwal, Yonglin Ren

Plant Protect. Sci., 2019, 55(1):54-60 | DOI: 10.17221/13/2018-PPS  

The use of stable isotopes to label an insect species, the Mediterranean fruit fly Ceratitis capitata (Diptera: Tephidae) (medfly) was investigated. Labelling allows mating and life history characteristics to be investigated experimentally. 13C6-glucose was incorporated into the diet of medflies at various stages of development by adding it to larval media or providing adults with sugar water. Data was collected from egg hatching until the death of adults. The results show that stable isotopes successfully labelled medflies in laboratory conditions. There were significant differences between labelled and unlabelled treatments...

Selection and evaluation of potential reference genes for gene expression analysis in Avena fatuaOriginal Paper

Junjie Liu, Peng Li, Liuyang Lu, Lanfen Xie, Xiling Chen, Baizhong Zhang

Plant Protect. Sci., 2019, 55(1):61-71 | DOI: 10.17221/20/2018-PPS  

Eight commonly used candidate reference genes, 18S ribosomal RNA (rRNA) (18S), 28S rRNA (28S), actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1 alpha (EF1α), ribosomal protein L7 (RPL7), Alpha-tubulin (α-TUB), and TATA box binding protein-associated factor (TBP), were evaluated under various experimental conditions to assess their suitability in different developmental stages, tissues and herbicide treatments in Avena fatua. The results indicated the most suitable reference genes for the different experimental...